RESUMO
Norovirus is a leading cause of acute gastroenteritis worldwide. The importance of this virus infection in Kuwait is not known. Eight out of 100 stool samples (8.0%) from children up to 5 years of age with gastroenteritis studied during 2006-2007 from one hospital, and 6 out of 70 stool samples (8.5%) from similar children studied from another hospital during 2010-2011 were positive for norovirus by RT-PCR. Out of these 170 samples studied from both hospitals, 10 samples were positive for norovirus when tested by ELISA. Phylogenetic tree analysis of norovirus strains showed that 50% of the norovirus strains belonged to genotype GII.4, and the predominant strain was GII.4 2006b. Other detected genotypes were GII.12, GII.b, GII.3, GII.8, and GII.7. This study highlights the importance of screening for norovirus infection in acute gastroenteritis and having a reporting system to understand better the epidemiology of norovirus infection in Kuwait.
Assuntos
Infecções por Caliciviridae/virologia , Gastroenterite/virologia , Variação Genética , Norovirus/classificação , Norovirus/genética , Infecções por Caliciviridae/epidemiologia , Pré-Escolar , Análise por Conglomerados , Gastroenterite/epidemiologia , Genótipo , Humanos , Lactente , Kuweit/epidemiologia , Masculino , Epidemiologia Molecular , Dados de Sequência Molecular , Norovirus/isolamento & purificação , Filogenia , Prevalência , RNA Viral/genética , Análise de Sequência de DNARESUMO
Epidemiological trends of the globally most common rotavirus genotype, G1P[8], were investigated in Hungary during a 16-year period by sequencing and phylogenetic analysis of the surface antigens. Antigen shift among epidemiologically major G1P[8] strains was observed in 6 seasons, as indicated by changes in the sublineages of the G1 VP7 and the P[8] VP4 genes. The temporal clustering of some rotavirus VP4 and VP7 gene sublineages and the periodic emergence and/or resurgence of previously unrecognized rotavirus sublineages in the study population suggest a dynamic nature for these common strains. Recently established international strain surveillance networks may help to identify and track the spread of epidemiologically important rotavirus strains across countries and continents.
Assuntos
Infecções por Rotavirus/epidemiologia , Rotavirus/genética , Substituição de Aminoácidos , Antígenos Virais/genética , Proteínas do Capsídeo/genética , Humanos , Hungria/epidemiologia , Rotavirus/classificação , Infecções por Rotavirus/virologia , Fatores de TempoRESUMO
BACKGROUND: The importance of diarrhoeagenic Escherichia coli (DEC) infections in the Arabian Gulf including Kuwait is not known. The prevalence of DEC (enterotoxigenic [ETEC], enteropathogenic [EPEC], enteroinvasive [EIEC], enterohemorrhagic [EHEC] and enteroaggregative [EAEC]) was studied in 537 children < or = 5 years old hospitalised with acute diarrhoea and 113 matched controls from two hospitals during 2005-07 by PCR assays using E. coli colony pools. RESULTS: The prevalence of DEC varied from 0.75% for EHEC to 8.4% for EPEC (mostly atypical variety) in diarrhoeal children with no significant differences compared to that in control children (P values 0.15 to 1.00). Twenty-seven EPEC isolates studied mostly belonged to non-traditional serotypes and possessed beta and theta intimin subtypes. A total of 54 DEC isolates from diarrhoeal children and 4 from controls studied for antimicrobial susceptibility showed resistance for older antimicrobials, ampicillin (0 to 100%), tetracycline (33 to 100%) and trimethoprim (22.2 to 100%); 43.1% of the isolates were multidrug-resistant (resistant to 3 or more agents). Six (10.4%) DEC isolates produced extended spectrum beta-lactamases and possessed genetic elements (blaCTX-M, blaTEM and ISEcp1) associated with them. CONCLUSION: We speculate that the lack of significant association of DEC with diarrhoea in children in Kuwait compared to countries surrounding the Arabian Gulf Region may be attributable to high environmental and food hygiene due to high disposable income in Kuwait.
Assuntos
Diarreia/etiologia , Infecções por Escherichia coli/epidemiologia , Escherichia coli/isolamento & purificação , Criança Hospitalizada , Pré-Escolar , Diarreia/epidemiologia , Diarreia/microbiologia , Farmacorresistência Bacteriana , Escherichia coli/classificação , Escherichia coli/genética , Escherichia coli/metabolismo , Infecções por Escherichia coli/microbiologia , Fezes/microbiologia , Genes Bacterianos , Humanos , Lactente , Recém-Nascido , Kuweit/epidemiologia , Testes de Sensibilidade Microbiana , Prevalência , Sorotipagem , beta-Lactamases/metabolismoRESUMO
Rotavirus strain surveillance is being conducted in many countries before and after introduction of newly licensed vaccines to assess the impact of the vaccines on rotavirus strains. Here we describe a strain surveillance study in the Budapest area of Hungary (2003-2006) based on RNA profile analysis, genotyping by multiplex PCR and nucleotide sequencing. Among 1,983 G-typed rotaviruses we identified G1 (22%), G2 (4.8%), G3 (3.5%), G4 (18.5%), G6 (1.1%), G8 (<0.1%, n = 1), G9 (42%), and G12 (3.4%) specificities. Information on P genotype incidence was determined for a subset of samples (n = 814). In addition to the globally important strains, a variety of uncommon antigen combinations were also found, for example, P[9],G3; P[14],G6; or P[14],G8. Sequence and phylogenetic analysis of the VP7, VP4, VP6, and NSP4 genes of selected strains with uncommon antigen combinations demonstrated high similarity with certain bovine, porcine, feline, equine, and lapine rotaviruses, respectively. Continued surveillance is needed to assess the role of animal rotaviruses in human diseases.
Assuntos
Variação Genética , Infecções por Rotavirus/epidemiologia , Infecções por Rotavirus/virologia , Rotavirus/genética , Zoonoses/virologia , Adolescente , Animais , Antígenos Virais/análise , Criança , Pré-Escolar , Fezes/virologia , Genótipo , Humanos , Hungria/epidemiologia , Lactente , Dados de Sequência Molecular , Filogenia , Rotavirus/classificação , Rotavirus/imunologia , Infecções por Rotavirus/genéticaRESUMO
The rotavirus non-structural protein NSP4 has been implicated in a number of biological functions during the rotavirus cellular cycle and pathogenesis, and has been addressed as a target for vaccine development. The NSP4 gene has been classified into six genotypes (A-F). A semi-nested triplex PCR was developed for genotyping the major human NSP4 genotypes (A-C), which are common in human rotavirus strains but are also shared among most mammalian rotavirus strains. A total of 192 previously characterized human strains representing numerous G and P type specificities (such as G1P[8], G1P[4], G2P[4], G3P[3], G3P[8], G3P[9], G4P[6], G4P[8], G6P[4], G6P[9], G6P[14], G8P[10], G8P[14], G9P[8], G9P[11], G10P[11], G12P[6] and G12P[8]) were tested for NSP4 specificity by the collaborating laboratories. An additional 35 animal strains, including the reference laboratory strains SA11 (simian, G3P[2]), NCDV (bovine, G6P[1]), K9 and CU-1 (canine, G3P[3]), together with 31 field isolates (canine, G3P[3]; feline, G3P[9]; porcine, G2P[23], G3P[6], G4P[6], G5P[6], G5P[7], G5P[26], G5P[27], G9P[6] and G9P[7]) were also successfully NSP4-typed. Four human G3P[9] strains and one feline G3P[9] strain were found to possess an NSP4 A genotype, instead of NSP4 C, suggesting a reassortment event between heterologous strains. Routine NSP4 genotyping may help to determine the genomic constellation of rotaviruses of man and livestock, and identify interspecies transmission of heterologous strains.
Assuntos
Glicoproteínas/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Rotavirus/classificação , Toxinas Biológicas/genética , Proteínas não Estruturais Virais/genética , Animais , Sequência de Bases , Gatos , Bovinos , Primers do DNA/química , DNA Complementar/química , Cães , Eletroforese em Gel de Ágar , Fezes/virologia , Genótipo , Haplorrinos , Humanos , Dados de Sequência Molecular , Filogenia , RNA Viral/genética , Reprodutibilidade dos Testes , Reação em Cadeia da Polimerase Via Transcriptase Reversa/normas , Rotavirus/genética , Especificidade da Espécie , SuínosRESUMO
Human adenovirus (HAdV) serotype 52 has recently been discovered in the United States in samples from human patients with gastroenteritis of unknown etiology and is suspected to be a new human enteric pathogen. The aim of the present pilot study was to investigate whether this virus is circulating in the population of Southern Hungary by screening stool specimens collected from gastroenteritis cases and communal sewage samples in the area of Baranya County. A total of 209 diarrheic stool (124 from children and 85 from adults) and 45 influent sewage samples were screened for HAdV-52 by PCR using a primer pair specific to the gene of 12.5K protein in the E3 genomic region. The novel human adenovirus was not detected in any of the tested samples, suggesting that HAdV-52 was not circulating in the target population and the area during the study period. Since temporal and geographical fluctuations may markedly affect the epidemiology of human enteric pathogens, additional investigations are required to gain more in-depth insights into the ecology of this novel adenovirus.
Assuntos
Infecções por Adenovirus Humanos/virologia , Adenovírus Humanos/genética , Gastroenterite/etiologia , Infecções por Adenovirus Humanos/complicações , Infecções por Adenovirus Humanos/epidemiologia , Adenovírus Humanos/classificação , Adenovírus Humanos/isolamento & purificação , Adulto , Criança , DNA Viral/análise , DNA Viral/genética , Fezes/virologia , Gastroenterite/epidemiologia , Humanos , Hungria/epidemiologia , Projetos Piloto , Reação em Cadeia da Polimerase , Sorotipagem , Esgotos/virologiaRESUMO
In rotaviruses, intragenic recombination or gene rearrangement occurs almost exclusively in the genome segments encoding for non-structural proteins. Rearranged RNA originates by mechanisms of partial sequence duplications and deletions or insertions of non-templated nucleotides. Of interest, epidemiological investigations have pointed out an unusual bias to rearrangements in genome segment 11, notably in rotavirus strains of lapine origin, as evidenced by the detection of numerous lapine strains with super-short genomic electropherotype. The sequence of the full-length genome segment 11 of two lapine strains with super-short electropherotype, LRV-4 and 3489/3, was determined and compared with rearranged and normal cognate genome segments of lapine rotaviruses. The rearranged genome segments contained head-to-tail partial duplications at the 3' end of the main ORF encoding NSP5. Unlike the strains Alabama and B4106, intermingled stretches of non-templated sequences were not present in the accessory RNA of LRV-4 and 3489/3, while multiple deletions were mapped, suggesting the lack of functional constraints. Altogether, these findings suggest that independent rearrangement events have given origin to the various lapine strains that have super-short genome pattern.
Assuntos
Rearranjo Gênico , Genoma Viral , Coelhos , Rotavirus/classificação , Rotavirus/genética , Animais , Sequência de Bases , Genes Virais , Infecções por Rotavirus/veterinária , Infecções por Rotavirus/virologiaRESUMO
UNLABELLED: Hepatitis E virus (HEV) is one of a common cause of acute, fecally transmitted hepatitis in developing countries. Identification of HEV in indigenous human infection and in domestic pig raises the possibility that HEV infection is also a zoonosis. AIM/METHODS: Molecular detection and epidemiology of HEV in humans with acute hepatitis and in domestic (pig, cattle) and wild (boar and roe-deer) animals by ELISA and RT-PCR in Hungary. RESULTS: Between 2001 and 2006, a total of 116 (9.6%) human sera were positive by HEV IgM ELISA and 13 (24.5%) of 53 samples were also confirmed by RT-PCR and sequencing. Forty-two, 11 and 9 samples were RT-PCR-positive from swine (feces: 22.7%; liver: 30.8%), roe-deer (liver: 34.4%) and wild boar (liver: 12.2%), respectively. Except for an imported infection caused by genotype 1, 19 sequences (human: 12, swine: 4, roe-deer: 1, wild boar: 2) belong to genotype 3 HEV. Genetically identical strains were detected in human and roe-deer and in 2 other human clusters. CONCLUSIONS: HEV is an endemic agent in Hungary. Consumption of raw or undercooked meat-products is one of the possible sources of the indigenous HEV infections. Cross-species infection with genotype 3 HEV involves a food-borne transmission route in Hungary.
Assuntos
Alimentos/virologia , Vírus da Hepatite E/genética , Hepatite E/epidemiologia , Zoonoses/transmissão , Zoonoses/virologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Criança , Pré-Escolar , Ensaio de Imunoadsorção Enzimática , Fezes/virologia , Feminino , Genótipo , Humanos , Hungria/epidemiologia , Lactente , Intestinos/virologia , Fígado/virologia , Masculino , Pessoa de Meia-Idade , RNA Viral/genética , RNA Viral/isolamento & purificação , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Adulto JovemRESUMO
Vaccination is the main strategy to control severe dehydrating gastroenteritis caused by rotaviruses in early childhood. The availability of new generation rotavirus vaccines has led to an intensification of strain surveillance worldwide, in part, to gauge the impact of the possible vaccine-driven immune selection of wild-type rotavirus strains. In the present study, authors describe the strain prevalence data obtained in 2007, with the involvement of different regions of Hungary. Genomic RNA was extracted from rotavirus-positive stool samples collected mainly from children and then subjected to genotyping using multiplex RT-PCR assay. Type-specific primers targeted G1 to G4, G6, G8 to G10, and G12 VP7 specificities, and P[4], P[6], and P[8] to P[11] VP4 specificities were used. Out of 489 rotavirus-positive specimens, collected from 482 patients, 466 and 474 were successfully G and P typed, respectively, and both G and P type specificities could be assigned for 457 strains. Prevalence data showed the predominance of G4P[8] (31.5%) strains, followed by G1P[8] (28.3%), G2P[4] (19.3%), and G9P[8] (10.2%). Minority strains were G1P[4] (0.4%), G2P[8] (1.3%), G3P[9] (0.2%), G4P[6] (0.7%), G6P[9] (0.4%), G8P[8] (0.2%), G9P[4] (0.2%), G9P[6] (0.8%), and G12P[8] (0.4%). Mixed infections were found in 1.2% of the samples, while 4.9% remained partially or fully non-typified. Our data indicate that the antigen specificities of medically important rotavirus strains identified in this 1-year study are well represented in the vaccines available in the pharmaceutical private market in Hungary. Depending on the vaccination coverage achievable in the forthcoming years, the post-vaccination rotavirus strain surveillance may allow us to gain comprehensive information on the impact of rotavirus vaccines on the prevalence of circulating rotavirus strains.
Assuntos
Gastroenterite/epidemiologia , Gastroenterite/virologia , Vigilância da População , Infecções por Rotavirus/epidemiologia , Infecções por Rotavirus/prevenção & controle , Vacinas contra Rotavirus/administração & dosagem , Rotavirus/isolamento & purificação , Pré-Escolar , Fezes/virologia , Feminino , Humanos , Hungria/epidemiologia , Lactente , Masculino , Reação em Cadeia da Polimerase , RNA Viral/isolamento & purificação , Rotavirus/genética , Rotavirus/imunologia , Estações do AnoRESUMO
Group C rotaviruses have been recognized as a cause of acute gastroenteritis in humans, cattle, and swine, although the true epidemiologic and clinical importance of this virus in these hosts has not yet been fully established. A real-time PCR assay based on a broadly reactive primer pair was developed and used to quantitatively determine the viral load of group C rotaviruses in environmental samples. A total of 35 raw and 35 treated sewage samples collected at the same sampling time in four Hungarian sewage treatment plants during a survey in 2005 were tested for the presence of group C rotaviruses. The overall detection rates were 91% (32 of 35) for the influent and 57% (20 of 35) for the effluent samples. Molecular characterization of the amplified partial VP6 gene revealed the cocirculation of human and animal (i.e., bovine and porcine) strains that were easily distinguishable by melting curve analysis. Human strains yielded relatively high viral loads (mean, 1.2 x 10(7); median, 6.9 x 10(5) genome equivalents per liter influent sewage) and appeared to display seasonal activity over the study period, whereas animal strains appeared to circulate throughout the year at much lower average titers (bovine strains mean, 9.9 x 10(4); median, 3.0 x 10(4); porcine strains mean, 3.9 x 10(4); median, 3.1 x 10(4) genome equivalents per liter influent sewage). Our findings suggest that monitoring of communal sewage may provide a good surrogate for investigating the epidemiology and ecology of group C rotaviruses in humans and animals.
Assuntos
Reação em Cadeia da Polimerase/métodos , Rotavirus/classificação , Rotavirus/isolamento & purificação , Esgotos/virologia , Virologia/métodos , Animais , Antígenos Virais/genética , Sequência de Bases , Proteínas do Capsídeo/genética , Primers do DNA/genética , Humanos , Dados de Sequência Molecular , Reprodutibilidade dos Testes , Rotavirus/genética , Estações do Ano , Temperatura de TransiçãoRESUMO
BACKGROUND: Noroviruses are common pathogens in human gastroenteritis outbreaks worldwide. They belong to a genetically diverse group of RNA viruses with multiple genogroups (G) and genotypes. Genotype GII-4 norovirus (Lordsdale) is the predominant agent in epidemics. OBJECTIVES: To investigate the genetic variation in GII-4 strains isolated during seven epidemic seasons in Hungary from November 2000 to June 2007. STUDY DESIGN: Using the prospective epidemiological surveillance of norovirus outbreaks in Hungary, GII-4 strains were selected for further genetic analysis. After phylogenetic analysis, RNA-polymerase (open reading frame 1; ORF1), capsid (ORF2) and the ORF1/ORF2 junction were analysed by RT-PCR and sequencing. RESULTS: Three hundred and seventy-seven (76.8%) of 491 confirmed norovirus outbreaks were caused by genotype GII-4. GII-4 was the predominant genotype in six of the seven epidemic seasons. Four main GII-4 variants--epidemic point mutants--(GII-4-2000, GII-4-2002, GII-4-2004 and GII-4-2006b) were detected, with each variant predominating in two consecutive epidemic seasons. CONCLUSIONS: Genotype GII-4 was confirmed as the predominant genetic type in epidemic norovirus seasons in Hungary. Genetic drift successfully promotes the re-emergence of GII-4 variants in the population. The elevated number of norovirus outbreaks in the population predicts the emergence of new GII-4 genetic variants as part of an international epidemic.
Assuntos
Infecções por Caliciviridae/epidemiologia , Surtos de Doenças , Gastroenterite/epidemiologia , Deriva Genética , Norovirus/classificação , Norovirus/genética , Infecções por Caliciviridae/virologia , Gastroenterite/virologia , Variação Genética , Genótipo , Humanos , Hungria/epidemiologia , Norovirus/isolamento & purificação , FilogeniaRESUMO
Tula hantavirus (TULV) is a member of the genus Hantavirus, family Bunyaviridae and is mainly carried by the European common vole (Microtus arvalis). In order to detect TULV, we tested Microtus arvalis (MAR) and Microtus subterraneus (MSU) voles captured in two different locations of the Southern Transdanubian region of Hungary. The viral genome was detectable in 37% of the tested MAR voles but, interestingly, was absent in all MSU. Phylogenetic analysis performed with a partial coding sequence of the capsid gene showed that Hungarian TULV strains clustered with viruses detected in western Slovakia and in the Czech Republic. To the best of our knowledge, this is the first report on the identification of TULV detected in MAR voles in the Transdanubian region of Hungary.
Assuntos
Arvicolinae/virologia , Infecções por Hantavirus/virologia , Orthohantavírus/isolamento & purificação , Animais , Orthohantavírus/classificação , Orthohantavírus/genética , Humanos , Hungria , Dados de Sequência Molecular , FilogeniaRESUMO
Dobrava hantavirus (DOBV) belongs to the genus Hantavirus of the family Bunyaviridae, and is carried by yellow necked and striped field mice (Apodemus flavicollis and Apodemus agrarius), respectively. The aim of this study was to detect and genetically characterize new DOBV strains in rodents captured in the Transdanubian region of Hungary. Rodent corpses were dissected and lung tissues were used for hantavirus detection by SYBR Green-based real-time RT-PCR using specific primers located in the S-segment of the virus genome. A total of 22 captured animals of the Apodemus species were tested for the presence of DOBV. Three out of the 22 mice were positive. Phylogenetic and molecular sequence analyses showed that Hungarian DOBVs were most closely related to those viruses detected from A. agrarius mice in Slovenia. Based on our new data from the region we concluded that extended reservoir studies would be necessary in the future.
Assuntos
Infecções por Hantavirus/veterinária , Murinae/virologia , Orthohantavírus/isolamento & purificação , Doenças dos Roedores/epidemiologia , Doenças dos Roedores/virologia , Animais , Benzotiazóis , Primers do DNA , Diaminas , Orthohantavírus/genética , Infecções por Hantavirus/epidemiologia , Infecções por Hantavirus/virologia , Hungria/epidemiologia , Dados de Sequência Molecular , Compostos Orgânicos/metabolismo , Filogenia , Quinolinas , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sequência de DNARESUMO
INTRODUCTION: Both infectious and non-infectious forms of acute conjunctivitis are known. Viruses, especially different types of adenoviruses are the etiological agents of infectious epidemic conjunctivitis (conjunctivitis epidemica). AIMS: The author's aims were to describe an outbreak of keratoconjunctivitis and to detect the viral agent by molecular methods in Hungary. MATERIALS AND METHODS: Classical epidemiological methods were used for investigation. Polymerase chain reaction (PCR) followed by sequencing were used for the detection of adenoviral hexon region from freshly collected conjunctival swabs. RESULTS: Between 9 October and 18 December 2006, a total of 60 patients became ill with keratoconjunctivitis in 7 settlements in Southwest Hungary. Mean age was 51,2 years. Conjunctivitis (100%), lacrimation (94%), foreign body sensation (83%), and dim vision (76%) were the main clinical symptoms. Both eyes were affected in half of the cases. Direct contact was the main transmission route including nosocomial spread associated with ophthalmology practices. Five (62.5%) of 8 conjunctival swabs were PCR-positive for adenovirus type 8 (HAdV8/Baranya/2006/HUN; EF210714) which was genetically identical to adenovirus strain detected in Austria in 2004 (DQ149614). CONCLUSIONS: The outbreak of keratoconjunctivitis was partially associated with nosocomial infection caused by type 8 adenovirus. Both the recognition of the clinical illness, laboratory diagnosis and public health measures are necessary for the prevention of keratoconjunctivitis infection and epidemic.
Assuntos
Infecções por Adenovirus Humanos/diagnóstico , Infecções por Adenovirus Humanos/epidemiologia , Adenovírus Humanos/isolamento & purificação , Conjuntivite Viral/diagnóstico , Conjuntivite Viral/epidemiologia , Surtos de Doenças , Ceratoconjuntivite/diagnóstico , Ceratoconjuntivite/epidemiologia , Doença Aguda , Infecções por Adenovirus Humanos/virologia , Adenovírus Humanos/genética , Adulto , Idoso , Conjuntivite Viral/virologia , Infecção Hospitalar/diagnóstico , Infecção Hospitalar/epidemiologia , Infecção Hospitalar/virologia , DNA Viral/isolamento & purificação , Feminino , Humanos , Hungria/epidemiologia , Ceratoconjuntivite/virologia , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Análise de Sequência de DNARESUMO
INTRODUCTION: Hepatitis A virus (HAV) is one of the most important cause of fecally transmitted acute infectious hepatitis worldwide. In Hungary, beside the sporadic HAV infections, outbreaks also occur, particularly in Northeast part of the country where the subgenotype IA is endemic. The reported number of HAV cases was less than 10 per year in Southwest Hungary. AIMS: A part of the European HAV surveillance, the author's aims were to follow and to analyze the outbreak of hepatitis A in Transdanubia (Southwest Hungary) in 2006 by molecular epidemiological methods. MATERIALS AND METHODS: Sera samples from symptomatic patients were tested prospectively by enzyme-immunoassay (EIA) and reverse transcription-polymerase chain reaction (RT-PCR) followed by sequence- and phylogenetic analysis. RESULTS: Between June and December 2006, a total of 115 serum samples were positive for HAV IgM antibody in Southwest Hungary. Thirty (76.9%) of the 39 samples were RT-PCR-positive and contain genetically identical subgenotype IB hepatitis A virus (HAV/Transdanubia/2006/HUN) which has 100% nucleotide identity to strain IT-MAR-02 from Italy in 2002. Until now, approximately 1200-1300 persons have been infected with the probably imported strain. The average age of patients was 18 years (years 1 to 80). In this study, detailed clinical and epidemiological data of the outbreak are presented. CONCLUSIONS: Only a prospective molecular epidemiological study could verify the connection between the first sporadic HAV cases in June, the outbreak in August and the endemic spread of the virus since September in Southwest Hungary. This epidemic calls attention to how a hepatitis A outbreak can develop anywhere and at any time in Hungary and to the importance of active HAV immunization in the primary prevention.
Assuntos
Surtos de Doenças , Vírus da Hepatite A/isolamento & purificação , Hepatite A/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Feminino , Vírus da Hepatite A/genética , Vírus da Hepatite A/imunologia , Humanos , Hungria/epidemiologia , Técnicas Imunoenzimáticas , Imunoglobulina M/sangue , Lactente , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
Group A rotaviruses are the most common cause of severe gastroenteritis worldwide. The incidence and distribution of group A rotavirus sero/genotypes varies between geographical areas during a rotavirus season, and from one season to the next. In addition, cocirculation of genetically diverse multitypic rotaviruses and of intratypic variants in any one place and time is common. Assuming widespread use of rotavirus vaccine in the near future, comprehensive surveillance of natural rotavirus infections is vital. EuroRotaNet has been established in order to gather comprehensive information on the rotavirus types co-circulating throughout Europe. The main objectives of the network are to (i) develop methods and algorithms for effective rotavirus strain typing and characterisation, (ii) describe in detail the molecular epidemiology of rotavirus infections in Europe, (iii) monitor the effectiveness of current genotyping methods and respond to changes associated with genetic drift and shift, and (iv) monitor the emergence and spread of novel rotavirus strains within Europe. This infrastructure may serve as a platform for future surveillance activities and nested studies for evaluating the effectiveness of a rotavirus vaccine in the general population. Studies to monitor the reduction in disease associated with common rotavirus types, the possible vaccine-induced emergence of antibody escape mutants of genotypes other than those included in the vaccine and of reassortment between vaccine and naturally circulating wildtype strains are required.
Assuntos
Redes Comunitárias , Infecções por Rotavirus/epidemiologia , Rotavirus , Europa (Continente) , Genótipo , Humanos , Hungria , Epidemiologia Molecular , Rotavirus/classificação , Rotavirus/genética , Infecções por Rotavirus/prevenção & controle , Infecções por Rotavirus/virologia , Vacinas contra Rotavirus/administração & dosagemRESUMO
BACKGROUND: Noroviruses are common pathogens in gastro-enteritis outbreaks in humans worldwide. Noroviruses are genetically diverse group of viruses with multiple genogroups (GG) and genotypes. More recently, naturally occurring recombinant noroviruses were described. These viruses had a distinct polymerase gene sequence (designated GGIIb/Hilversum) and were disseminated through waterborne and food-borne transmission in Europe. OBJECTIVES: Our aim was to characterize these emerging recombinant noroviruses causing outbreaks of gastro-enteritis in Hungary. STUDY DESIGN: From January 2001 to May 2004, samples containing "GGIIb/Hilversum polymerase" (GGIIb-pol) were selected for analysis of the viral capsid region (ORF2) by reverse transcription-polymerase chain reaction (RT-PCR) and sequencing. RESULTS: Thirty-four (14.4%) of 236 confirmed norovirus outbreaks were caused by the variant lineage with the GGIIb-pol. Four different recombinants were detected with capsids of Hu/NLV/GGII/Mexico/1989 (n=9, 43%), Hu/NLV/GGII/Snow Mountain/1976 (n=6, 28%), Hu/NLV/GGII/Hawaii/1971 (n=4, 19%) and Hu/NLV/GGII/Lordsdale/1993 (n=1, 5%). CONCLUSIONS: In Hungary, emerging recombinant noroviruses became the second most common norovirus variants-next to GGII-4/Lordsdale virus-causing epidemics of gastroenteritis in the last 4 years.
Assuntos
Infecções por Caliciviridae/epidemiologia , Capsídeo/classificação , Doenças Transmissíveis Emergentes/epidemiologia , Surtos de Doenças , Gastroenterite/epidemiologia , Norovirus/classificação , Recombinação Genética , Infecções por Caliciviridae/transmissão , Infecções por Caliciviridae/virologia , Doenças Transmissíveis Emergentes/transmissão , Doenças Transmissíveis Emergentes/virologia , Gastroenterite/virologia , Humanos , Hungria/epidemiologia , Epidemiologia Molecular , Dados de Sequência Molecular , Norovirus/genética , Norovirus/isolamento & purificação , Filogenia , Análise de Sequência de DNARESUMO
First human case of hepatitis E infection detected in Hungary is reported. This hepatitis E virus (HEV), Hungary1, belongs to genotype 3 and had 95% and 90% nucleotide identity within the capsid region of the European swine and human (Greece2) strains, respectively. Hungary1 represents a potential novel human variant of HEV in genus Hepevirus.
Assuntos
Vírus da Hepatite E/classificação , Hepatite E/virologia , Feminino , Genes Virais , Vírus da Hepatite E/genética , Humanos , Hungria , Pessoa de Meia-Idade , Dados de Sequência Molecular , Fases de Leitura Aberta , Homologia de Sequência do Ácido Nucleico , Especificidade da Espécie , ViagemRESUMO
BACKGROUND: Highly publicised outbreaks of norovirus gastroenteritis in hospitals in the UK and Ireland and cruise ships in the USA sparked speculation about whether this reported activity was unusual. METHODS: We analysed data collected through a collaborative research and surveillance network of viral gastroenteritis in ten European countries (England and Wales were analysed as one region). We compiled data on total number of outbreaks by month, and compared genetic sequences from the isolated viruses. Data were compared with historic data from a systematic retrospective review of surveillance systems and with a central database of viral sequences. FINDINGS: Three regions (England and Wales, Germany, and the Netherlands) had sustained epidemiological and viral characterisation data from 1995 to 2002. In all three, we noted a striking increase in norovirus outbreaks in 2002 that coincided with the detection and emergence of a new predominant norovirus variant of genogroup II4, which had a consistent mutation in the polymerase gene. Eight of nine regions had an annual peak in 2002 and the new genogroup II4 variant was detected in nine countries. Also, the detection of the new variant preceded an atypical spring and summer peak of outbreaks in three countries. INTERPRETATION: Our data from ten European countries show a striking increase and unusual seasonal pattern of norovirus gastroenteritis in 2002 that occurred concurrently with the emergence of a novel genetic variant. In addition to showing the added value of an international network for viral gastroenteritis outbreaks, these observations raise questions about the biological properties of the variant and the mechanisms for its rapid dissemination.
Assuntos
Infecções por Caliciviridae/epidemiologia , Surtos de Doenças/estatística & dados numéricos , Gastroenterite/epidemiologia , Norovirus/isolamento & purificação , Infecções por Caliciviridae/transmissão , Infecções por Caliciviridae/virologia , Europa (Continente)/epidemiologia , Microbiologia de Alimentos , Gastroenterite/virologia , Variação Genética , Humanos , Mutação/genética , Norovirus/genética , Vigilância da População , Estudos Retrospectivos , Estações do AnoRESUMO
Application of improved molecular techniques in the detection and characterization of rotavirus strains has led to the recent description of several new combinations, specificities, and genetic variants of the outer capsid genes, VP7 and VP4. In spite of the enormous diversity of mammalian rotavirus strains, the few lapine rotaviruses characterized to date, appear to carry a narrow range of such antigen combinations; only P[14], G3 and, based on a more recent study, P[22], G3 rotaviruses have proved to be epidemiologically important in rabbits. In the present study, we characterized a lapine group A rotavirus with a super-short electropherotype detected in an outbreak of fatal enteritis in a Hungarian commercial rabbitry. Based on sequence and phylogenetic analysis of the VP7, VP4, and NSP4 genes, our lapine strain is a P[22], G3 rotavirus that carries the NSP4 genotype shared by most lapine rotaviruses. Although the P[22] VP4 specificity has been newly identified, the relatively high sequence variation between our strain and those identified in Italy (89.1-90.4% nucleotide identity; region VP8*) implies that these strains diversified far before they were described for the first time, strongly suggesting that this genotype may have circulated in rabbitries or in nature without prior detection. We conclude that genotype P[22] lapine rotaviruses show a wider geographical dispersal than previously thought, although understanding their true epidemiological significance needs further investigation.