Latest Trends in Electrochemical Sensors for Neurotransmitters: A Review.

Neurotransmitters are endogenous chemical messengers which play an important role in many of the brain functions, abnormal levels being correlated with physical, psychotic and neurodegenerative diseases such as Alzheimer's, Parkinson's, and Huntington's disease. Therefore, their sensitive and robust detection is of great clinical significance. Electrochemical methods have been intensively used in the last decades for neurotransmitter detection, outclassing more complicated analytical techniques such as conventional spectrophotometry, chromatography, fluorescence, flow injection, and capillary electrophoresis. In this manuscript, the most successful and promising electrochemical enzyme-free and enzymatic sensors for neurotransmitter detection are reviewed. Focusing on the activity of worldwide researchers mainly during the last ten years (2010-2019), without pretending to be exhaustive, we present an overview of the progress made in sensing strategies during this time. Particular emphasis is placed on nanostructured-based sensors, which show a substantial improvement of the analytical performances. This review also examines the progress made in biosensors for neurotransmitter measurements in vitro, in vivo and ex vivo.

Fabrication of an ultrasensitive and selective electrochemical aptasensor to detect carcinoembryonic antigen by using a new nanocomposite.

A lable-free electrochemical aptasensor was successfully developed for the sensitive detection of carcinoembryonic antigen as a tumor biomarker. To do this, a ternary nanocomposite of hemin, graphene oxide and multi-walled carbon nanotubes was used. The aptamer can be attached to the surface of a hemin, graphene oxide and multi-walled carbon nanotubes glassy carbon electrode through -NHCO- covalent bonds to form a sensing surface. Through fourier transform infrared spectroscopy and scanning electron microscopy, it was indicated that hemin can be successfully incorporated into hemin, graphene oxide and multi-walled carbon nanotubes. Hemin, which protects graphene nanosheets, also serves as an in-situ probe owing to its well-defined redox properties. Multi-walled carbon nanotubes in the modifier enhance conductivity and facilitate the electron transfer between hemin and the glassy carbon electrode. In this study, carcinoembryonic antigen got specifically bound to the aptamer, and the current changes were used for selective and specific detection of that antigen. The devised aptasensor proved to have excellent performance with a wide linear range of 1.0 × 10-15 - 1.0 × 10-8 gmL-1 and a detection limit of 0.82 fg mL-1. The inter-day and intra-day values of RSD% were obtained in the range of 0.10-2.91 and 2.21-4.56 respectively. According to the experiments conducted on real samples, it may be claimed that the proposed label-free electrochemical aptasensor is capable enough of determining carcinoembryonic antigen in clinical diagnostics.