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1.
J Exp Med ; 179(3): 1053-8, 1994 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-7906704

RESUMO

The etiopathogenesis of focal and segmental glomerular sclerosis (FGS) remains unknown. Using a new animal model for FGS (FGS mouse), we demonstrate here that bone marrow transplantation from normal mice to FGS mice with a high grade of proteinuria (+ + +) ameliorates FGS, and that the transplantation of bone marrow cells or purified hemopoietic stem cells (HSCs) from FGS mice induces FGS in normal mice. These findings strongly suggest that FGS is a stem cell disorder; the abnormalities may be genetically programmed at the level of HSCs.


Assuntos
Transplante de Medula Óssea , Glomerulosclerose Segmentar e Focal/patologia , Transplante de Células-Tronco Hematopoéticas , Células-Tronco Hematopoéticas/patologia , Animais , Transplante de Medula Óssea/patologia , Transplante de Medula Óssea/fisiologia , Quimera , Modelos Animais de Doenças , Glomerulosclerose Segmentar e Focal/terapia , Antígenos H-2/análise , Teste de Histocompatibilidade , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos , Proteinúria , Baço/imunologia
2.
Exp Hematol ; 28(8): 950-60, 2000 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-10989196

RESUMO

Intrathymic T-cell differentiation is characterized by two selection events: positive and negative selection. It has been shown that thymic epithelial cells in the cortex are involved in the positive selection, while macrophages and dendritic cells, derived from hemopoietic stem cells, are involved in the negative selection. Here we investigate whether donor-derived bone marrow stromal cells can migrate into the thymus and participate there in positive selection after bone marrow transplantation plus bone grafts (to recruit bone marrow stromal cells). Allogeneic bone marrow transplantation with or without bone grafts was carried out in the [C57BL/6-->C3H] combination. Fluorescence-activated cell sorter analyses of recipient thymic adherent cells showed that donor-type bone marrow stromal cells exist in the thymus of mice that received bone marrow plus bone grafts but not in the mice that received bone marrow cells alone. Histological examination using confocal microscopy also confirmed the existence of donor-type stromal cells in the thymus of mice that received bone marrow cells plus bones. Both T-cell proliferation and plaque-forming cell assays indicated that the T cells of such mice show donor-type major histocompatibility complex-restriction. These findings strongly suggest that stromal cells can migrate from the bone marrow to the thymus, where they participate in the positive selection of thymocytes.


Assuntos
Células da Medula Óssea/citologia , Transplante de Medula Óssea , Transplante Ósseo , Células Estromais/citologia , Células Estromais/fisiologia , Timo/citologia , Animais , Células Apresentadoras de Antígenos/imunologia , Antígenos/imunologia , Adesão Celular , Movimento Celular , Antígenos H-2/imunologia , Humanos , Ativação Linfocitária , Complexo Principal de Histocompatibilidade , Camundongos , Camundongos Endogâmicos C3H , Camundongos Endogâmicos C57BL , Microscopia Confocal , Linfócitos T/imunologia , Doadores de Tecidos
3.
Transplantation ; 71(12): 1725-31, 2001 Jun 27.
Artigo em Inglês | MEDLINE | ID: mdl-11455249

RESUMO

A new strategy for organ allografts that does not require recourse to immunosuppressants is established in mice. The strategy includes sublethal (7 Gy) irradiation followed by the injection of donor bone marrow cells (BMCs) via the portal vein (P.V.) and organ allografts 1 day after irradiation. Irradiation doses (< or =7 Gy) are found to allow the recipients to survive without the need to reconstitute the BMCs, as the recipient hematolymphoid cells can gradually recover. One hundred percent of recipients irradiated with 7 Gy followed by either P.V. or i.v. injection of donor BMCs accept organ allografts (the skin, pancreas, and adrenal glands) for more than 1 year. However, organ allograft survival rates decrease when irradiation doses are reduced; the skin graft survival rate of mice treated with 6.5 Gy and P.V. injection of BMCs is 79%, whereas that of mice treated with 6.5 Gy and i.v. injection is 50%, indicating that the P.V. injection of BMCs induces persistent tolerance more effectively than the i.v. injection. H-2 typing reveals that almost all the hematolymphoid cells (>98%) in the peripheral blood and hematolymphoid organs are donor-derived even 1 year after the treatment (7 Gy and P.V.). The T cells are tolerant to both donor-type and host-type MHC determinants. The major mechanism underlying the persistent tolerance induced by this strategy seems to be because of clonal deletion. This simple and safe strategy would be of great advantage for human organ transplantation.


Assuntos
Transplante de Medula Óssea/métodos , Transplante de Órgãos , Veia Porta , Cuidados Pré-Operatórios , Irradiação Corporal Total , Glândulas Suprarrenais/transplante , Animais , Quimera , Feminino , Sobrevivência de Enxerto , Tolerância Imunológica , Injeções Intravenosas , Camundongos , Camundongos Endogâmicos , Transplante de Pâncreas , Transplante de Pele , Análise de Sobrevida , Imunologia de Transplantes , Transplante Homólogo
4.
Cancer Lett ; 153(1-2): 101-8, 2000 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-10779637

RESUMO

Apoptosis is clearly distinguished from necrosis, morphologically and chemically. Morphologically, apoptosis is characterized by a condensed nucleus and the disappearance of microvilli without disruption of the cytoplasm. In this report, we demonstrate that 5-fluorouracil (5-FU)-induced early apoptotic cells are characterized by (i) ultracondensed mitochondria, (ii) no change in the microvilli or nucleus, (iii) a high mitochondrial transmembrane potential (Deltapsi(m)), and (iv) being annexin V(negative). The early apoptotic cells also show the active forms of caspase 8 and caspase 9. They rapidly lose Deltapsi(m) after further incubation. Therefore, we conclude that the ultracondensation of mitochondria precedes the loss of Deltapsi(m) and the exposure of phosphatidylserine to the outer leaflet of the cell membrane.


Assuntos
Antimetabólitos Antineoplásicos/farmacologia , Apoptose , Caspases/metabolismo , Fluoruracila/farmacologia , Mitocôndrias/ultraestrutura , Adenocarcinoma , Núcleo Celular/ultraestrutura , Neoplasias Colorretais , Ativação Enzimática , Humanos , Potenciais da Membrana/efeitos dos fármacos , Mitocôndrias/efeitos dos fármacos , Células Tumorais Cultivadas
5.
Immunobiology ; 197(1): 1-15, 1997 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-9241527

RESUMO

Fetal (days 15 to 17) organs such as the small intestine, stomach and pancreas were engrafted under the renal capsules of athymic nude (nu/nu) mice to examine the capacity of these organs to induce the differentiation of T cells. Eight weeks after engraftment, the engrafted organs had differentiated into adult-type organs histologically. In the lamina propria of the engrafted small intestine, large intestine, and stomach, there were clusters of lymphocytes or lymphoid follicles, which included Thy1.2+ or CD4+ T cells. Flow cytometric analyses revealed that the lymphocytes from the lymph nodes of sham-, esophagus-, or pancreas-engrafted mice included very few T cells (1.20%), whereas those from the lymph nodes of the fetal small intestine-, large intestine-, or stomach-engrafted mice included significant numbers of T cells (8.36%) 8 weeks after engraftment, although there were not as many as in the fetal thymus-engrafted mice (17.97%). The peripheral T cells in the small intestine-, large intestine-, or stomach-engrafted mice were of bone marrow origin, and consisted of Thy1.2+, CD3+, and CD4+8-, or CD4-8+ with T cell receptor (TcR) alpha beta cells. Taken together, these findings indicate that not only the murine small intestine and large intestine but also the stomach have the capacity to induce the differentiation of T cells.


Assuntos
Feto/imunologia , Linfócitos T/citologia , Timo/citologia , Animais , Antígenos Ly/genética , Transplante de Medula Óssea/imunologia , Diferenciação Celular/imunologia , Esôfago/transplante , Transplante de Tecido Fetal/imunologia , Citometria de Fluxo , Intestino Grosso/transplante , Intestino Delgado/transplante , Tecido Linfoide/citologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C3H , Camundongos Endogâmicos C57BL , Camundongos Nus , Transplante de Pâncreas/imunologia , Estômago/transplante , Linfócitos T/transplante
6.
Immunobiology ; 188(1-2): 99-112, 1993 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-8406562

RESUMO

MRL/lpr mice spontaneously develop rheumatoid arthritis (RA)-like disease. Recently we have observed that bone marrow transplantation (BMT) with bone graft to recruit donor stromal cells can be used to treat autoimmune diseases (including RA-like lesions) in MRL/lpr mice. In this paper, we characterize the origin of synovial cells with the use of radiation chimeras and elucidate the repairing mechanism of RA by BMT. Type A synoviocytes have been thought to play an important role in the initiation of inflamed synovia, since a large number of Type A synoviocytes have been seen in inflamed synovia of both RA patients and MRL/lpr mice. Using [C57BL/6JJic-bg-->MRL/lpr] chimeric mice, we found Type A synoviocytes to be derived from donor bone marrow cells. They appeared in the inflamed synovia 4 weeks after BMT. However, at this time, the repairing process was not prominent. Serial biopsy studies revealed that newly developed T cells with normal functions play a more crucial role in the treatment of RA in MRL/lpr mice than do Type A synoviocytes.


Assuntos
Artrite Reumatoide/patologia , Artrite Reumatoide/terapia , Transplante de Medula Óssea/patologia , Membrana Sinovial/patologia , Animais , Artrite Reumatoide/fisiopatologia , Doenças Autoimunes/patologia , Doenças Autoimunes/fisiopatologia , Doenças Autoimunes/terapia , Transplante de Medula Óssea/fisiologia , Divisão Celular , Células Cultivadas , Inflamação , Camundongos , Camundongos Endogâmicos C3H , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos , Receptores de IgG/análise , Fatores de Tempo , Transplante Homólogo
7.
Immunobiology ; 192(5): 279-96, 1995 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-7649564

RESUMO

We have recently found that allogeneic bone marrow transplantation (BMT) can be used to treat lupus nephritis in (NZB x NZW)F1(B/WF1), BXSB, MRL/lpr and (NZW x BXSB)F1 mice. To elucidate why and how glomerular damage is repaired by BMT, serial renal biopsies were carried out using B/WF1 mice before and after BMT. Donor-derived B cells and macrophages with normal functions developed two weeks (wks) after BMT. At this stage, the macrophages did not show immune complex (IC) clearance activity. Donor-derived T cells with normal functions were generated six wks after BMT. At this stage, visceral epithelial cells macrophages and mesangial cells in the glomeruli were activated by T cells and showed marked phagocytic activity; macrophages and mesangial cells were found to be responsible for the clearance of ICs, whereas, to our surprise, epithelial cells were found to be responsible for the repair of injured basement membranes. These findings suggest that T cells with normal functions, which have the capacity to activate macrophages, mesangial cells and epithelial cells, play a crucial role in repairing IC-mediated glomerular damage.


Assuntos
Transplante de Medula Óssea/fisiologia , Nefrite Lúpica/fisiopatologia , Nefrite Lúpica/terapia , Linfócitos T/fisiologia , Animais , Complexo Antígeno-Anticorpo/análise , Glomérulos Renais/imunologia , Glomérulos Renais/patologia , Glomérulos Renais/ultraestrutura , Camundongos , Camundongos Endogâmicos NZB , Transplante Homólogo/fisiologia
8.
Immunobiology ; 192(5): 365-81, 1995 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-7649568

RESUMO

Effects of irradiation and deoxyguanosine on the fetal thymus were examined both in vitro and in vivo. Fetal thymi (gestation day 15) of C57BL/6 mice that had been irradiated (0-25 Gy) or treated with various doses of deoxyguanosine (dGuo) were engrafted under the renal capsules of BALB/c nu/nu mice, and the differentiation of T cells was investigated in the engrafted thymi or spleens of these mice. After in vitro treatment of fetal thymi with 1.35 mM dGuo (which was previously reported to be an optimal dose), T cell precursors still remained in some cultures, whereas 1.80 mM dGuo was highly cytotoxic not only to T cell precursors but also to thymic epithelial cells. In contrast, 25 Gy irradiation totally eliminated the T cell precursors from the fetal thymi, though the capacity of epithelial cells to induce T cell differentiation was retained. Although irradiated thymi had the capacity to induce T cell differentiation when assayed in an in vitro organ culture system, long-term observation of thymi engrafted into BALB/c nu/nu mice revealed that, if they had been irradiated (9.5 Gy or 25 Gy), the thymi became scarred by 12 wks after their transplantation. Furthermore, the expression of cell interaction molecules such as ICAM-1 and MHC class II on the thymus stromal cells decreased after irradiation. The interaction molecules decreased 3 wks after 25 Gy irradiation and 7 wks after 9.5 Gy irradiation. The alteration in T cell subsets in the thymus (decreases in both double- and single- positive cells and an increase in double-negative cells) correlated with the decreases in the interaction molecules. This indicates that irradiation (even 9.5 Gy) impairs the T cell-induction capacity of the thymus stromal cells, resulting in an alteration of the T cell subsets followed by a change in the T cell counts in the thymus. Therefore, the long-term effects of irradiation of the thymus should be considered in cases of fetal thymus grafts or total body irradiation before bone marrow transplantation, particularly in the newborn.


Assuntos
Desoxiguanosina/toxicidade , Timo/efeitos dos fármacos , Timo/efeitos da radiação , Animais , Desenvolvimento Embrionário e Fetal/efeitos dos fármacos , Desenvolvimento Embrionário e Fetal/efeitos da radiação , Feminino , Antígenos de Histocompatibilidade Classe II/análise , Molécula 1 de Adesão Intercelular/análise , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Camundongos Nus , Técnicas de Cultura de Órgãos , Gravidez , Timo/transplante , Irradiação Corporal Total
9.
Immunobiology ; 201(5): 515-26, 2000 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-10834310

RESUMO

The role of the thymic microenvironment in the development of murine thymic B cells has yet to be fully clarified. We therefore investigate the microenvironment that supports the development of mature thymic B cells (sIg+/B220+/CD43-B cells) from thymic B cell progenitors with immunophenotypes of sIg-/B220med/CD43+ cells. As we have previously reported, thymic B cells generated from these progenitors in the thymus are CD5+ B cells. We next study the in vitro condition that supports the differentiation of thymic B cell progenitors. Stromal cells (from the bone marrow or thymus), thymus-derived cell lines with the character of thymic nurse cells (TNCs) or thymic epithelial cells (TECs), or the bone marrow-derived cell line (MS-5) are tested for their ability to support B-lymphopoiesis from thymic B cell progenitors. Interestingly, thymic stromal cells (but neither stromal cells from the bone marrow nor stromal cell lines) support the differentiation of thymic B cell progenitors into thymic B cells in the presence of IL-7. Cortical epithelia (but not medullary epithelia, thymic macrophages or dendritic cells) are found to contribute to thymic B cell differentiation. Surface phenotype and Ig rearrangement analyses reveal that mature B cells generated in this condition are primarily CD5+ B cells, indicating that the thymic microenvironment (particularly cortical epithelia) determines the differentiation of thymic B cells.


Assuntos
Antígenos CD , Linfócitos B/citologia , Células-Tronco/citologia , Timo/citologia , Animais , Linfócitos B/imunologia , Células da Medula Óssea/imunologia , Antígenos CD5/análise , Comunicação Celular , Diferenciação Celular/efeitos dos fármacos , Células Cultivadas , Células Epiteliais/imunologia , Feminino , Citometria de Fluxo , Imuno-Histoquímica , Interleucina-7/farmacologia , Antígenos Comuns de Leucócito/análise , Leucossialina , Masculino , Camundongos , Camundongos Endogâmicos C3H , Receptores de Antígenos de Linfócitos B/análise , Sialoglicoproteínas/análise , Células Estromais/citologia , Células Estromais/metabolismo , Timo/imunologia , Fatores de Tempo
10.
Immunobiology ; 186(5): 449-65, 1992 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-1286883

RESUMO

To clarify the mechanism by which the lpr gene causes lymphadenopathy, we established an experimental system to induce lymph node (LN) swelling in unaffected mice. In MRL-(+)/+ mice that had been 5 Gy-irradiated and grafted with bone marrow cells (BMCs) plus LN from MRL-lpr/lpr mice, a remarkable enlargement of the LN grafts was seen. The enlarged grafts lacked normal LN structure and were indistinguishable from LNs of MRL-lpr/lpr mice. The induction of LN swelling by this method was achieved not only in [MRL-lpr/lpr-->MRL-(+)/+] but also in [MRL-lpr/lpr-->BALB/c], [MRL-lpr/lpr-->C3H], [B6-lpr/lpr-->B10.Thy1.1], and [B6-lpr/lpr-->BALB/c] combinations. Furthermore, the lpr/lpr LN grafts developed lymph node swelling even without the transplantation of BMCs. Most cells in the grafted LNs disappeared within a few days, and large clear fibroblast-like cells then became dominant for 1 to 4 weeks. Thereafter, lymphoid cells increased and had filled the graft by the 8th week. The LN grafts obtained from MRL-lpr/lpr (but not MRL-(+)/+) mice showed the ability to transfer LN node swelling into the secondary MRL-(+)/+ hosts two weeks after the primary transplantation. These results strongly suggest that the fibroblast-like LN stromal cells play a crucial role in lpr-associated lymphadenopathy.


Assuntos
Doenças Autoimunes/genética , Genes , Linfonodos/patologia , Doenças Linfáticas/etiologia , Transtornos Linfoproliferativos/genética , Camundongos Endogâmicos/genética , Camundongos Mutantes/genética , Animais , Doenças Autoimunes/imunologia , Transplante de Medula Óssea/imunologia , Fibroblastos/patologia , Genótipo , Hipertrofia , Imunoterapia Adotiva , Linfonodos/imunologia , Linfonodos/transplante , Doenças Linfáticas/imunologia , Doenças Linfáticas/patologia , Transtornos Linfoproliferativos/imunologia , Camundongos , Camundongos Endogâmicos BALB C/genética , Camundongos Endogâmicos BALB C/imunologia , Camundongos Endogâmicos/imunologia , Camundongos Mutantes/imunologia , Fenótipo , Quimera por Radiação
11.
Immunobiology ; 197(1): 31-43, 1997 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-9241529

RESUMO

The male (NZW x BXSB)F1 (W/BF1) mouse, a murine model for autoimmune diseases, shows hepatosplenomegaly with lymphoid cell infiltration in the liver by 20 weeks of age. The majority of infiltrating cells are T cells, B cells and plasma cells, as seen in autoimmune hepatitis. Together with the increase in serum glutamate pyruvate transaminase (GPT) levels, anti-dsDNA antibody (Ab) and circulating immune complex (CIC) levels increase with age. These findings are compatible with those of autoimmune hepatitis in humans. In addition, a unique finding in this mouse is the accumulation of CD4+ Mac-1+ Class II+ cells in the sinusoidal space. The cells have the capacity to proliferate and differentiate into macrophages in vitro, indicating that they are the precursors of macrophages. This W/BF1 mouse provides a useful tool for not only analyzing the pathogenesis of autoimmune hepatitis but also establishing a new therapeutic strategy for it. In addition, we discuss the significance of the appearance of abnormal cells in autoimmune-prone mice.


Assuntos
Doenças Autoimunes/etiologia , Antígenos CD4/análise , Hepatite Animal/imunologia , Antígenos de Histocompatibilidade Classe II/análise , Fígado/imunologia , Antígeno de Macrófago 1/análise , Animais , Doenças Autoimunes/imunologia , Doenças Autoimunes/patologia , Diferenciação Celular/imunologia , Movimento Celular/imunologia , Separação Celular , Células Clonais , Cruzamentos Genéticos , Modelos Animais de Doenças , Citometria de Fluxo , Hepatite Animal/etiologia , Hepatite Animal/patologia , Imunofenotipagem , Fígado/patologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos NZB
12.
Am J Clin Pathol ; 96(2): 262-6, 1991 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-1862781

RESUMO

An autopsy case of I-cell disease in a 4-year-old Japanese girl is presented. In this report, the authors analyze the relationship between morphologic (including electron microscopic) and biochemical findings. Lymph node, spleen, and kidney, which were stained with Hale's colloidal iron method, contained large amounts of hexosamine. These substances had accumulated in lymphocytes of B-cell lineage.


Assuntos
Mucolipidoses/patologia , Cadáver , Pré-Escolar , Feminino , Hexosaminas/metabolismo , Humanos , Imuno-Histoquímica , Rim/metabolismo , Linfonodos/metabolismo , Linfócitos/metabolismo , Microscopia Eletrônica , Mucolipidoses/metabolismo , Baço/metabolismo
13.
Arch Otolaryngol Head Neck Surg ; 126(6): 782-4, 2000 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-10864117

RESUMO

We report the first case of an ectopic pituitary adenoma in the nasal cavity that produced follicle-stimulating hormone (FSH). A 60-year-old man complaining of left nasal bleeding had a polypoid tumor in the left nasal cavity. Findings of computed tomographic scanning and magnetic resonance imaging showed that the tumor originated from the olfactory cleft, occupied the nasal cavity, and extended to the frontal cranial fossa. Results of histologic examination suggested ectopic pituitary adenoma. Magnetic resonance imaging results showed the pituitary gland to be normal. Electron microscopy findings demonstrated a large number of secretory granules in the tumor cells that were positive for FSH on immunohistochemical analyses. Serum gonadotropin levels were normal, and no clinical signs of hypersecretory syndrome were noted. The above findings led us to establish the diagnosis of FSH-producing ectopic pituitary adenoma. The patient underwent craniofacial resection of the tumor followed by an uneventful recovery. The pathologic findings and clinical course of the case were comparable to those of FSH-producing adenomas arising from the pituitary gland.


Assuntos
Adenoma/metabolismo , Coristoma/metabolismo , Hormônio Foliculoestimulante/metabolismo , Cavidade Nasal , Neoplasias Nasais/metabolismo , Hipófise , Base do Crânio/patologia , Adenoma/diagnóstico por imagem , Adenoma/patologia , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Cavidade Nasal/diagnóstico por imagem , Invasividade Neoplásica , Neoplasias Nasais/diagnóstico por imagem , Neoplasias Nasais/patologia , Tomografia Computadorizada por Raios X
14.
Exp Toxicol Pathol ; 45(1): 29-34, 1993 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-8467197

RESUMO

A cloned cell line was established from tumor cells spontaneously developed in a coculture of an autoreactive T cell line (1/+ T1) and 30 Gy-irradiated MRL/+ spleen cells with Con A supernatants. Morphological studies and studies of histamine content and modes of histamine release after stimulation with compound 48/80 revealed that the cell line (MRL-MC3) had mast cell characteristics. MRL-MC3 was transplantable not only to MRL/+, MRL/lpr and AKR/J (H-2k) mice but also to BALB/c and (BALB/c x DBA/2) F1 (H-2d) mice, although the allogeneic mice survived twice as long as syngeneic mice after i.v. injection. In addition, after i.v. injection, the mast cells infiltrated the livers and spleens of syngeneic (MRL/+) mice, however the lymph nodes around the mesenterium to the parapylorus in allogeneic (BALB/c) mice. A mast cell line (BALB-MC) was also established from a lymph node of MRL-MC3-injected BALB/c mice. Cell surface marker analyses revealed clear differences between the BALB-MC and the original MRL-MC3, which was positive for the expression of MHC class I antigens (K, D), I-E antigen and c-abl-encoded (anti-pEX-2 antibody-reactive) proteins, but not for I-A on the cell surface. In contrast, BALB-MC showed positive only for the MHC class I antigens (K, D) on the surface, and also positive for anti-pEX-2 antibody-reactive cytoplasmic proteins, as seen in MRL-MC3. Mast cells obtained from MRL-MC3-injected MRL/+ mice showed the same staining pattern as MRL-MC3. BALB-MC induced shorter survival times (approximately half) in both MRL/+ and BALB/c mice than MRL-MC3.(ABSTRACT TRUNCATED AT 250 WORDS)


Assuntos
Antígenos de Histocompatibilidade/análise , Mastócitos/imunologia , Mastócitos/transplante , Transplante de Neoplasias , Animais , Antígenos de Neoplasias/análise , Antígenos de Superfície/análise , Camundongos , Camundongos Endogâmicos BALB C , Células Tumorais Cultivadas
15.
Hinyokika Kiyo ; 36(10): 1185-8, 1990 Oct.
Artigo em Japonês | MEDLINE | ID: mdl-2176059

RESUMO

A case of phylloides tumor of the prostate in a 58-year-old male is presented. The tumor was composed of columnar cystic folds and pleomorphic stromal elements including bizarre giant cells. Electron microscopic examination, which was performed using specimens embedded in paraffin blocks, revealed that the bizarre giant cells originated from the smooth muscle. The postoperative course was uneventful, with no evidence of local recurrence or metastasis for more than 2 years after operation.


Assuntos
Células Gigantes/patologia , Tumor Filoide/patologia , Neoplasias da Próstata/patologia , Células Gigantes/ultraestrutura , Humanos , Masculino , Pessoa de Meia-Idade , Músculo Liso/patologia , Tumor Filoide/cirurgia , Tumor Filoide/ultraestrutura , Neoplasias da Próstata/cirurgia , Neoplasias da Próstata/ultraestrutura
16.
Kyobu Geka ; 53(6): 450-6, 2000 Jun.
Artigo em Japonês | MEDLINE | ID: mdl-10846355

RESUMO

The autopsy of a 76-year-old Japanese female patient, which revealed thymic carcinoma with various tumor markers such as NSE, CYFRA, and CA-125, is presented. The patient died from hepatic failure because the liver was overtaken by the tumors. At autopsy, the thymic carcinoma was found to have metastased only in the liver. From microscopical analyses and electron microscopical findings, we diagnosed poorly differenciated squamous cell carcinoma of thymic origin. In the histochemical analyses, the tumor cells were positively stained in CA 125, CA 19-9, EMA, NSE, AE 1, AE 3, CEA, S-100, glimerius and Bcl-2. These date suggest that the tumor cells produced various tumor markers. In 222 autopsy cases of thymic malignant tumor observed in Japan over a period of 4 years, the dominant pathohistological image was squamous cell carcinoma. It is interesting that the greatest number of combined malignant tumors with thymic malignancies were thyroid papillary carcinomas.


Assuntos
Biomarcadores Tumorais/análise , Antígeno CA-19-9/análise , Carcinoma de Células Escamosas/secundário , Neoplasias Hepáticas/secundário , Neoplasias do Timo/patologia , Idoso , Antígeno Ca-125/análise , Antígeno Carcinoembrionário/análise , Carcinoma de Células Escamosas/patologia , Feminino , Humanos , Neoplasias Hepáticas/patologia , Fosfopiruvato Hidratase/análise
17.
Acta Pathol Jpn ; 36(5): 715-32, 1986 May.
Artigo em Inglês | MEDLINE | ID: mdl-3739706

RESUMO

Of a total of 80 offspring obtained by reciprocal crossing of ICR/JCL strain of mice of both sexes receiving 84-140 injections of Fe-NTA over 98-163 days before crossing, 52 (65%) showed severe generalized amyloidosis after 93-502 injections of Fe-NTA over 115-522 days, but not "hemochromatosis", which was a striking contrast to the findings of "hemochromatosis" without amyloidosis observed in the parent mice. Electron microscopic examinations revealed numerous bundles of non-branching, well-oriented amyloid fibrils radiated outward from the surface of cytoplasmic invaginations of the Kupffer cells or splenic reticuloendothelial cells of the F1 mice with amyloidosis, and close contact frequently observed between "amyloid-forming cells" and adjacent lymphocytes in the amyloid-laden liver and spleen of the F1 mice. Since the above findings in the F1 mice were not found in the parent mice treated with multiple Fe-NTA injections, the present authors assumed that the immunological memory for the Fe-NTA conjugate transmitted via the placenta to the fetus from the mother that received multiple Fe-NTA injections might be involved in the development of generalized amyloidosis in the F1 mice, although the possible mechanism by which Fe-NTA-induced "F1 amyloidosis" has been developed remains yet undetermined.


Assuntos
Amiloidose/patologia , Compostos Férricos , Ferro , Ácido Nitrilotriacético/análogos & derivados , Amiloidose/induzido quimicamente , Amiloidose/genética , Animais , Cruzamentos Genéticos , Modelos Animais de Doenças , Feminino , Compostos Férricos/administração & dosagem , Histocitoquímica , Injeções Intraperitoneais , Ferro/administração & dosagem , Nefropatias/patologia , Hepatopatias/metabolismo , Hepatopatias/patologia , Masculino , Camundongos , Camundongos Endogâmicos ICR , Microscopia Eletrônica , Linhagem , Esplenopatias/metabolismo , Esplenopatias/patologia
18.
Acta Pathol Jpn ; 36(3): 399-413, 1986 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-3716794

RESUMO

The histological distribution and ultrastructural findings were investigated in 52 amyloid-positive cases obtained from 80 F1 mice (32 males and 48 females) receiving 126 to 502 daily intraperitoneal injections of ferric nitrilotriacetic acid (Fe-NTA) resulting from reciprocal crossing of 20 parental mice receiving daily intraperitoneal injections of Fe-NTA for 5 months. Of 52 amyloidotic F1 mice 49 (94%) developed a moderate degree of amyloid deposits in the gastrointestinal tract. Moderate amounts of amyloid deposits were sporadically discernible in the lamina propria of the stomach pars glandularis, the duodenal mucosa, and to a lesser extent in that of the rectal mucosa. Electron microscopic observation revealed that macrophages adjacent to amyloid mass were radiating outward abundant bundles of non-branching amyloid fibrils from the cytoplasmic invaginations. In the cytoplasm of the macrophages there were occasionally acid phosphatase-positive lysosomes including amyloid fibrils measuring approximately 100 A in width. Moreover, it is discussed whether fibroblasts or fibroblast-like interstitial cells are involved in amyloid formation.


Assuntos
Amiloidose/patologia , Doenças do Sistema Digestório/patologia , Ácido Nitrilotriacético/análogos & derivados , Amiloide/metabolismo , Amiloidose/induzido quimicamente , Amiloidose/genética , Animais , Doenças do Sistema Digestório/induzido quimicamente , Doenças do Sistema Digestório/genética , Feminino , Compostos Férricos , Hibridização Genética , Masculino , Camundongos , Camundongos Endogâmicos ICR , Microscopia Eletrônica , Linhagem
19.
Blood ; 83(4): 964-71, 1994 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-7509214

RESUMO

Chemotactic factors produced by stromal cells in the bone marrow are characterized. Two kinds of factors produced by stromal cell lines are identified using blind-well Boyden's Chambers; one is a neutrophil-chemotactic factor and the other a hematopoietic stem cell (HSC)-chemotactic factor. The latter attracts blastic cells in a low-density fraction, which are Thy1lo, wheat germ agglutinin (WGA)hi, H-2Khi, Ly-1-, Ly-2-, L3T4-, Ly5-, and slg-. The molecular weight of this HSC-chemotactic factor is estimated to be more than 200 kD. Putative cytokines and growth factors, such as granulocyte colony-stimulating factor (CSF), macrophage CSF, granulocyte-macrophage CSF, stem cell factor (SCF), interleukin (IL)-6, and IL-3, do not possess HSC-chemotactic activity. These findings strongly suggest that bone marrow stromal cells produce a new factor that attracts HSCs.


Assuntos
Medula Óssea/metabolismo , Fatores Quimiotáticos/biossíntese , Quimiotaxia , Citocinas/farmacologia , Substâncias de Crescimento/farmacologia , Células-Tronco Hematopoéticas/fisiologia , Animais , Antígenos de Superfície/análise , Antígenos de Superfície/biossíntese , Células da Medula Óssea , Linhagem Celular , Células Cultivadas , Fatores Quimiotáticos/análise , Quimiotaxia/efeitos dos fármacos , Ensaio de Unidades Formadoras de Colônias , Meios de Cultivo Condicionados , Feminino , Fator Estimulador de Colônias de Granulócitos/farmacologia , Fator Estimulador de Colônias de Granulócitos e Macrófagos/farmacologia , Fatores de Crescimento de Células Hematopoéticas/farmacologia , Células-Tronco Hematopoéticas/efeitos dos fármacos , Humanos , Interleucina-3/farmacologia , Interleucina-6/farmacologia , Cinética , Fator Estimulador de Colônias de Macrófagos/farmacologia , Glicoproteínas de Membrana/análise , Glicoproteínas de Membrana/biossíntese , Camundongos , Camundongos Endogâmicos C3H , Proteínas Recombinantes/farmacologia , Fator de Células-Tronco , Antígenos Thy-1
20.
Stem Cells ; 18(4): 273-80, 2000.
Artigo em Inglês | MEDLINE | ID: mdl-10924093

RESUMO

We examined the induction of tolerance using pancreas allografts over the long term (>1 year) in mice for the human application of mixed allogeneic bone marrow transplantation (BMT). T cell-depleted BM cells (BMCs) of C57BL/6 (B6) and C3H/He (C3H) mice were transplanted at various ratios into lethally irradiated B6 mice. The percentages of C3H cells in the chimeric mice gradually decreased, finally declining to only a small percentage, except when the ratio of donor to recipient BMCs was 100:1. However, despite the marked decreases in C3H-type cells, all the pancreas allografts of C3H mice were accepted when more than 1% C3H cells were detected in the peripheral blood. To examine the relationships between percentages of transplanted donor cells and acceptance of pancreas allografts, various percentages of donor and recipient BMCs (5% to 30%) were transplanted. It was found that more than 10% donor cells were necessary for the pancreas allografts to be accepted. In vitro assays for mixed lymphocyte reaction and generation of cytotoxic T-lymphocytes revealed that spleen cells in chimeric mice accepting pancreas allografts are tolerant to both host-type and donor-type major histocompatibility complex (MHC) determinants, but show a vigorous responsiveness to third-party MHC determinants. Since donor-type hemopoietic stem cells (HSCs) were detected in the BM and the liver of the chimeric mice, donor-derived HSCs and donor-derived hematolymphoid cells are responsible for the induction of tolerance. It should be noted that the percentage of donor-type HSCs is higher in the liver (6.2%) than in the BM (0.9%).


Assuntos
Transplante de Medula Óssea/imunologia , Tolerância Imunológica/imunologia , Transplante de Pâncreas/imunologia , Quimeras de Transplante/imunologia , Animais , Feminino , Fígado/citologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C3H , Camundongos Endogâmicos C57BL , Fatores de Tempo , Transplante Homólogo
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