RESUMO
Invasive species that outcompete endemic ones and toxic harmful algae that cause algal blooms threaten marine resources like fisheries, aquaculture, and even tourism. Environmental DNA (eDNA) metabarcoding can help as a method for early alert. In this study, we have analyzed communities inhabiting six lagoons within the Gulf of Lion (northwest Mediterranean Sea) with spatial protection as RAMSAR and Natura 2000 sites. Employing the COI gene as the only metabarcode, we found 15 genera that have caused recognized algal bloom outbreaks in the studied lagoons since 2000. In addition, seven alien invasive species that can pose risks to the rich marine resources of the zone and lagoons were also found. The results found from eDNA are consistent with events of toxic algae blooms before and after the sampling moment and with reported occurrences of the invasive species in nearby Mediterranean areas. Multivariate multiple analysis showed the importance of anthropic pressure in the abundance of these nuisance species. Mitigation actions and routine eDNA metabarcoding in zones of special interest like these fragile French Mediterranean lagoons are recommended for early alert of nuisance species in order to plan timely management actions.
Assuntos
DNA Ambiental , Monitoramento Ambiental , Espécies Introduzidas , Mar Mediterrâneo , DNA Ambiental/genética , DNA Ambiental/análise , Monitoramento Ambiental/métodos , Código de Barras de DNA Taxonômico , Proliferação Nociva de Algas , AnimaisRESUMO
Macroalgae are able to retain environmental microplastics (MPs). The potential ingestion of MP through Atlantic agar Gelidium corneum and different animal species (hake, glass eels, mussels, topshells, anemones, sea cucumbers) that are seafood resources in Spain, was estimated from published MPs data calculating daily dose and annual ingestion rate. The study region was Asturias (SW Bay of Biscay). Lower MP ingestion rate from algae than from any animal analysed revealed a reduced risk of MP intake, probably because the alga is harvested from quite clean subtidal zones. However, MP bioconcentration in Atlantic agar was higher than in sea cucumbers, mussels or glass eels. Compared with other algae, G. corneum ranked the highest for MP retention rate, perhaps for its intricate branching and gelatinous surface, suggesting a possible use in MP bioremediation. More experimental studies in MP uptake by macroalgae are recommended to understand their implication in the accumulation of this pollutant.
Assuntos
Monitoramento Ambiental , Microplásticos , Alimentos Marinhos , Poluentes Químicos da Água , Animais , Poluentes Químicos da Água/análise , Espanha , Alga MarinhaRESUMO
The richness of plankton communities determines the fish productivity in the ocean, including important resources that rely on extractive fisheries, such as hakes (genus Merluccius) and tunas (genus Thunnus). Their preys forage on zooplankton, and the latter feed on phytoplankton. Inventories of plankton communities for scientific advice to sustainable fishing are essential in this moment of climate change. Plankton is generally inventoried using conventional methodologies based on large water volumes and visual morphological analyses of samples. In this study, we have employed metabarcoding on environmental DNA (eDNA) samples extracted from small water volumes for plankton inventory from twelve distant sampling stations in the East Atlantic Ocean. Zones rich in hake and tuna prey were detected from eDNA, and multivariate multiple regression analysis was able to predict those zones from diatom-based indices and planktonic diversity based on functional groups. Salinity was negatively correlated with the proportion of diatoms in phytoplankton, highlighting expected impacts of current global change on marine plankton communities. The results emphasise the importance of the plankton richness for fish productivity and support the utility of environmental DNA as a tool to monitor plankton composition changes.
Assuntos
DNA Ambiental , Diatomáceas , Animais , Plâncton , Atum , Fitoplâncton/genética , Água , EcossistemaRESUMO
Early detection of invasive species is crucial to deal effectively with biological invasions in ports, which are hotspots of species introductions. In this study, a simplified end-time PCR methodology conducted on eDNA from water samples was developed for rapid detection of the invasive seaweed Asparagopsis armata (four hours from water collection to result visualization). It was tested dockside in four international Spanish ports in presence of stakeholders, whose feedback was obtained to explore the real applicability of this biotechnology. Although biological invasions were not a main concern for them, results indicate a unanimous approval of the methodology by the stakeholders, having detected the presence of A. armata in three of the ports. Stakeholders suggested further developments for easier application of the tool and multiple species detection, to be adopted for the control of invasive species in ports.
Assuntos
Rodófitas , Alga Marinha , Alga Marinha/genética , Rodófitas/genética , Espécies Introduzidas , ÁguaRESUMO
In the present work, derivatives of phenanthridine-6(5H)-ones and benzo[c]chromenes were efficiently prepared through an intramolecular C-H bond functionalization reaction catalyzed by photochemically synthesized Pd-PVP nanoparticles. The heterocycles were obtained via intramolecular arylation of the corresponding N-methyl-N-aryl-2-halobenzamide or aryl-(2-halo)benzyl ethers using K2CO3 as base in a mixture of H2O : DMA as solvent without additives or ligands. High yields of the heterocyclic compounds were achieved (up to 95%) using a moderately low catalyst loading (1-5 mol%) under an air atmosphere at 100 °C. The reaction exhibited very good tolerance to diverse functional groups (OMe, Me, t Bu, Ph, OCF3, CF3, F, Cl, -CN, Naph), and both bromine and iodine substrates showed great reactivity. Finally, the in vitro antiproliferative activity of phenanthridine-6(5H)-ones and benzo[c]chromenes was evaluated against six human solid tumor cell lines. The more active compounds exhibit activity in the low micromolar range. 1-Isopropyl-4-methyl-6H-benzo[c]chromene was identified as the best compound with promising values of activity (GI50 range 3.9-8.6 µM). Thus, the benzochromene core was highlighted as a novel organic building block to prepare potential antitumor agents.