Two unusual novel iridoid glycosides from Cornus officinalis fruit and their biological activities.

Two unusual novel iridoid glycosides, cornsecoside A (1) and cornsecoside B (2), were isolated from a 40% ethanol elution fraction of a 50% ethanol extract of Cornus officinalis fruit. Their structures were determined by spectroscopic data analysis combined with hydrolysis and ECD spectroscopy. In addition, compounds 1 and 2 exhibited cytotoxic activity against Bel-7402 cells with IC50 values of 8.12 and 9.31 µM, and were neuroprotective against H2O2-induced SH-SY5Y cell injure at a concentration of 10 µM.

[HPLC Characteristic Fingerprint of "Boju" Chrysanthemum morifolium].

OBJECTIVE: To provide a scientific basis for quality control of "Boju" Chrysanthemum morifolium by establishing a HPLC characteristic fingerprint. METHODS: The HPLC analysis was performed on a Spursil C18 chromatographic column (250 mm x 4. 6 mm, 5 µm), and the mobile phase was acetonitrile-0. 1% phosphoric acid in a gradient mode with the flow rate of 1. 0 mL/min. The detection wavelength was 325 nm and the temperature of column was 30 °C. RESULTS: The common pattern of HPLC characteristic chromatographic profile was established. There were 16 common peaks, five of which were identified in the pattern. The similarities of 10 batches of "Boju" Chrysanthemum monrfolium were evaluated, and all of them were greater than 0. 900. CONCLUSION: This analysis method of HPLC characteristic chromatographic fingerprint is simple and reproducible, and it can provide a scientific basis for identification and quality evaluation of "Boju" Chrysanthemum monfolium.

[Determination of Trace Elements in Different Parts of Boju and Root Soil by ICP-MS].

OBJECTIVE: To explore the contents and enrichment status of the trace elements in Boju by using ICP-MS. METHODS: Trace elements of the different parts of Boju and the soil near the roots were determined by microwave digestion-ICP-MS and the correlation of the data obtained was tested by using the statistical software. RESULTS: There were differences among the contents of the eight elements in the different parts of Boju and the soil; there were obvious differences in the enrichment factor of eight elements in the different parts of Boju, which ranged from 0.0054 to 0.9205; there was a significant positive correlation between Fe and Mg in the inflorescence and the contents of Fe was positively correlated with the soil; for Al, Ba and Mg, there were significant positive correlations between its soil contents and leaves contents. CONCLUSION: Boju absorbs the inorganic elements selectively,the yield and quality of Chinese medicinal materials can be improved by improving the cultivation.

[Experiences of Professor Zhou Zhong-ying in diagnosing and treating rheumatoid arthritis by data mining].

OBJECTIVE: To summarize Professor ZHOU Zhong-ying's academic ideas and clinical experiences in diagnosing and treating rheumatoid arthritis (RA). METHODS: Totally 153 clinical case reports were recruited. Data mining techniques like frequencies, factor analysis, and association analysis were used in order to find out laws of syndrome, pathogenesis, treatment methods, formulae and medication. RESULTS: By statistical analyses, we found 14 main symptoms, 76 commonly used clinical drugs, 8 core prescriptions for RA, and the correlation between main pathogeneses and related drugs. CONCLUSION: Results analyzed and summarized from mining his experiences in RA treatment were in line with clinical practice.