RESUMO
Brassica napus is an important oil crop and cold stress severely limits its productivity. To date, several studies have reported the regulatory genes and pathways involved in cold-stress responses in B. napus. However, transcriptome-scale identification of the regulatory genes is still lacking. In this study, we performed comparative transcriptome analysis of cold-tolerant C18 (CT - C18) and cold-sensitive C6 (CS - C6) Brassica napus genotypes under cold stress for 7 days, with the primary purpose of identifying cold-responsive transcription in B. napus. A total of 6061 TFs belonging to 58 families were annotated in the B. napus genome, of which 3870 were expressed under cold stress in both genotypes. Among these, 451 TFs were differentially expressed (DE), with 21 TF genes expressed in both genotypes. Most TF members of the MYB (26), bHLH (23), and NAC (17) families were significantly expressed in the CT - C18 genotype compared with the CS - C6 B. napus genotype. GO classification showed a significant role in transcription regulation, DNA-binding transcription factor activity, response to chitin, and the ethylene-activated signaling pathway. KEGG pathway annotation revealed these TFs are involved in regulating more pathways, resulting in more tolerance. In conclusion, the results provide insights into the molecular regulation mechanisms of B. napus in response to freezing treatment, expanding our understanding of the complex molecular mechanisms in plants' response to freezing stress.
Assuntos
Brassica napus , Transcriptoma , Humanos , Transcriptoma/genética , Brassica napus/metabolismo , Fatores de Transcrição/metabolismo , Regulação da Expressão Gênica de Plantas/genética , Perfilação da Expressão Gênica/métodos , GenótipoRESUMO
Malonyl-CoA serves as the main building block for the biosynthesis of many important polyketides, as well as fatty acid-derived compounds, such as biofuel. Escherichia coli, Corynebacterium gultamicum, and Saccharomyces cerevisiae have recently been engineered for the biosynthesis of such compounds. However, the developed processes and strains often have insufficient productivity. In the current study, we used enzyme-engineering approach to improve the binding of acetyl-CoA with ACC. We generated different mutations, and the impact was calculated, which reported that three mutations, that is, S343A, T347W, and S350W, significantly improve the substrate binding. Molecular docking investigation revealed an altered binding network compared to the wild type. In mutants, additional interactions stabilize the binding of the inner tail of acetyl-CoA. Using molecular simulation, the stability, compactness, hydrogen bonding, and protein motions were estimated, revealing different dynamic properties owned by the mutants only but not by the wild type. The findings were further validated by using the binding-free energy (BFE) method, which revealed these mutations as favorable substitutions. The total BFE was reported to be -52.66 ± 0.11 kcal/mol for the wild type, -55.87 ± 0.16 kcal/mol for the S343A mutant, -60.52 ± 0.25 kcal/mol for T347W mutant, and -59.64 ± 0.25 kcal/mol for the S350W mutant. This shows that the binding of the substrate is increased due to the induced mutations and strongly corroborates with the docking results. In sum, this study provides information regarding the essential hotspot residues for the substrate binding and can be used for application in industrial processes.
Assuntos
Acetil-CoA Carboxilase , Streptomyces antibioticus , Acetil-CoA Carboxilase/genética , Acetil-CoA Carboxilase/metabolismo , Streptomyces antibioticus/metabolismo , Acetilcoenzima A/genética , Simulação de Acoplamento Molecular , Mutação , Saccharomyces cerevisiae/metabolismo , Escherichia coli/metabolismoRESUMO
Microplastics pose significant challenges to ecosystems and organisms. They can be ingested by marine and terrestrial species, leading to potential health risks and ecological disruptions. This study aims to address the urgent need for effective remediation strategies by focusing on the biodegradation of microplastics, specifically polyvinyl chloride (PVC) derivatives, using the bacterial strain Bacillus albus. The study provides a comprehensive background on the accumulation of noxious substances in the environment and the importance of harnessing biodegradation as an eco-friendly method for pollutant elimination. The specific objective is to investigate the enzymatic capabilities of Bacillus albus, particularly the alpha/beta hydrolases (ABH), in degrading microplastics. To achieve this, in-silico studies were conducted, including analysis of the ABH protein sequence and its interaction with potential inhibitors targeting PVC derivatives. Docking scores of - 7.2 kcal/mol were obtained to evaluate the efficacy of the interactions. The study demonstrates the promising bioremediation prospects of Bacillus albus for microplastics, highlighting its potential as a key player in addressing microplastic pollution. The findings underscore the urgent need for further experimental validation and practical implementation of Bacillus albus in environmental remediation strategies.
Assuntos
Bacillus , Biodegradação Ambiental , Cloreto de Polivinila , Bacillus/enzimologia , Bacillus/metabolismo , Cloreto de Polivinila/química , Hidrolases/metabolismo , Proteínas de Bactérias/metabolismo , Microplásticos/metabolismo , Simulação de Acoplamento MolecularRESUMO
Food authentication and contamination are significant concerns, especially for consumers with unique nutritional, cultural, lifestyle, and religious needs. Food authenticity involves identifying food contamination for many purposes, such as adherence to religious beliefs, safeguarding health, and consuming sanitary and organic food products. This review article examines the issues related to food authentication and food fraud in recent periods. Furthermore, the development and innovations in analytical techniques employed to authenticate various food products are comprehensively focused. Food products derived from animals are susceptible to deceptive practices, which can undermine customer confidence and pose potential health hazards due to the transmission of diseases from animals to humans. Therefore, it is necessary to employ suitable and robust analytical techniques for complex and high-risk animal-derived goods, in which molecular biomarker-based (genomics, proteomics, and metabolomics) techniques are covered. Various analytical methods have been employed to ascertain the geographical provenance of food items that exhibit rapid response times, low cost, nondestructiveness, and condensability.
Assuntos
Contaminação de Alimentos , Animais , Humanos , Análise de Alimentos/métodos , Contaminação de Alimentos/análise , Metabolômica/métodos , Proteômica/métodosRESUMO
Cadmium (Cd) is a highly toxic metal that frequently contaminates our environment. In this study, the bioflocculant-producing, cadmium-resistant Escherichia fergusonii ZSF-15 was characterized from Paharang drain, Bawa Chak, Faisalabad, Pakistan. The Cd-resistant E. fergusonii was used to determine the bioflocculant production using yeast-peptone-glycerol medium (pH 6.5) supplemented with 50 mg L-1 of Cd. The culture was incubated for 3 days at 37 °C in a rotary shaker at 120 rpm. The fermentation broth was centrifuged at 4000 g for 10 min after the incubation period. The maximum flocculating activity by isolate ZSF-15 was found to be 71.4% after 48 h of incubation. According to the Fourier transform infrared spectroscopy analysis, the bioflocculant produced by strain ZSF-15 was comprised of typical polysaccharide and protein, i.e. hydroxyl, carboxyl, and amino groups. The strain ZSF-15 exhibited bioflocculant activity at range of pH (6-8) and temperature (35-50â). Maximum flocculation activity (i.e. 71%) was observed at 47â, whereas 63% flocculation production was observed at pH 8. In the present study, antioxidant enzyme profile of ZSF-15 was also evaluated under cadmium stress. A significant increase in antioxidant enzymes including superoxide dismutase (118%) and ascorbate peroxidase (28%) was observed, whereas contents of catalase (86%), glutathione transferase (13%), and peroxidase (8%) were decreased as compared to control.
Assuntos
Antioxidantes , Cádmio , Escherichia , Cádmio/toxicidade , Concentração de Íons de Hidrogênio , Monitoramento Ambiental , FloculaçãoRESUMO
Bullying remains a pervasive issue that affects many children worldwide, with devastating consequences that ripple through their lives and communities [...].
RESUMO
Plant growth and development is adversely affected by environmental constraints, particularly salinity and drought. Climate change has escalated the effect of salinity and drought on crops in varying ways, affecting agriculture and most importantly crop productivity. These stressors influence plants across a wide range of levels, including their morphology and physiological, biochemical, and molecular processes. Plant responses to salinity and drought stress have been the subject of intense research being explored globally. Considering the importance of the impact that these stresses can have on agriculture in the short term, novel strategies are being sought and adopted in breeding programs. Better understanding of the molecular, biochemical, and physiological responses of agriculturally important plants will ultimately help promote global food security. Moreover, considering the present challenges for agriculture, it is critical to consider how we can effectively transfer the knowledge generated with these approaches in the laboratory to the field, so as to mitigate these adversities. The present collection discusses how drought and salinity exert effects on plants.
Assuntos
Secas , Salinidade , Melhoramento Vegetal , Produtos Agrícolas/fisiologia , Desenvolvimento VegetalRESUMO
Graphite, with its exceptional cyclic performance, continues to dominate as the preferred anode material for lithium-ion batteries. However as high-energy application gains momentum, there is growing demand for higher capacities that alloying/de alloying and conversion type anode materials can offer. Despite their potential, these materials are plagued by challenges such as volumetric fluctuations, low conductivities, and poor cyclic stability. Carbon nanostructures, on the other hand, show tremendous promise with their low volume expansion, high ion diffusion rates, and excellent conductivity. Nevertheless, their limited areal and volumetric densities restrict their widespread utilization. To address these limitations, various strategies such as doping, composite formation, and structural modification have been proposed. This article provides a succinct overview of carbon nanomaterials and their electrochemical performance as 3D carbon-based anodes, along with a comprehensive analysis of the strategies employed to overcome associated challenges while evaluating their potential prospects in the field.
RESUMO
The excessive accumulation of heavy metals, particularly lead (Pb) in agricultural soils, is a growing problem worldwide and needs urgent attention. This study aimed to prepare green silicon (Si) NPs using extract of Chenopodium quinoa leaves and evaluated their effects on Pb uptake and growth of maize (Zea mays L.). The results indicated that Pb exposure negatively affected the growth and chlorophyll contents of maize varieties, while SiNPs positively affected these attributes. Pb alone increased the electrolyte-leakage (EL), hydrogen-peroxide (H2O2) and selected antioxidant enzyme activities in leaves, whereas SiNPs decreased EL and H2O2 concentrations and further enhanced the enzyme activities as compared to their respective treatments without SiNPs. Pb-only treatments led to an increase in Pb concentrations and total Pb uptake in both shoots and roots. In contrast, SiNPs resulted in reduced Pb concentrations, with a concurrent decrease in total Pb uptake in shoots compared to the control treatment. The findings demonstrated that foliar application of SiNPs can mitigate the toxic effects of Pb in maize plants by triggering the antioxidant enzyme system and reducing the oxidative stress. Taken together, SiNPs have the potential to enhance maize production in Pb-contaminated soils. However, future research and application efforts should prioritize key aspects such as optimizing NPs synthesis, understanding positive mechanisms of green-synthesized NPs, and conducting multiple crop tests and real-world field trials.
Assuntos
Nanopartículas , Poluentes do Solo , Zea mays , Antioxidantes/farmacologia , Silício/farmacologia , Chumbo/toxicidade , Peróxido de Hidrogênio/farmacologia , Nanopartículas/toxicidade , Solo , Poluentes do Solo/análiseRESUMO
The present research investigates the effectiveness of nano-emulsified coatings (C-1, C-2, and C-3) in preserving the kiwifruit at a temperature of 10 ± 2 °C with 90-95 % relative humidity (RH) for 30 days. The nano-emulsions were prepared from varied carboxymethyl cellulose (CMC) concentrations with different combinations of essential oils such as thyme, clove, and cardamom. Dynamic light scattering investigation with Zeta Sizer revealed that C-1, C-2, and C-3 nano-emulsions have nano sizes of 81.3 ± 2.3, 115.3 ± 4.2, and 63.2 ± 3.2 nm, respectively. The scanning electron microscopy images showed that the nanoemulsion of C-1 had homogenous spherical globules, C-2 had voids, and C-3 showed a non-porous structure with uniform dispersion. The X-ray diffraction analysis indicated that C-1, C-2, and C-3 nano-emulsion exhibited distinct crystallinity and peaks. The nano-emulsion C-1 had reduced crystallinity, while C-2 had lower intensity peaks, and C-3 had increased crystallinity. The results documented that compared to control kiwifruit samples, the samples coated with C-3 nano-emulsion have decreased weight loss, decay incidence, soluble solids, maturity index activity, ethylene production, total bacterial count, and increased titratable acid, and firmness attributes. The results of current research are promising and would be applicable in utilization in industrial applications.
Assuntos
Filmes Comestíveis , Óleos Voláteis , Conservação de Alimentos/métodos , Carboximetilcelulose Sódica/química , Temperatura , Emulsões/químicaRESUMO
Arsenic is a highly toxic metal that causes cancer even at a low concentration and its removal from water resources is challenging. Herein, carbon extracted from waste onion bulbs is activated to cater for porosity and functionalized with magnetite (Fe3O4) nanoparticles (named MCK6) to address the challenge of As(III) removal. Synthesized MCK6 was highly mesoporous having a surface area of 208 m2/g, where magnetite nanoparticles (≤ 10 nm) are homogeneously distributed within a porous network. The developed adsorbent inherited functional groups from the biosource and magnetic property from magnetite making it ideal for removal of As(III). Further, MCK6 showed a maximum monolayer adsorption capacity (qm) of 10.2 mg/g at 298 K and pH 7. The adsorption thermodynamics delineates a non-spontaneous and endothermic reaction, where the kinetics followed pseudo 2nd order (R2 value of 0.977), while monolayer formation is explained by the Langmuir model. Moreover, MCK6 efficiently works to remove As(III) in a competitive metal ions system including Pb+2, Cd+2, and Ca+2, making it a suitable adsorbent to tackle contaminated water.
Assuntos
Nanopartículas de Magnetita , Poluentes Químicos da Água , Purificação da Água , Água/química , Óxido Ferroso-Férrico , Porosidade , Carbono , Poluentes Químicos da Água/análise , Adsorção , Cinética , Concentração de Íons de Hidrogênio , Nanopartículas de Magnetita/químicaRESUMO
Chronic pulmonary aspergillosis is a lung disorder characterized by the presence of single or multiple cavities with or without an aspergilloma or nodules on chest imaging, with mycological evidence of and/or demonstration of immunological response to Aspergillus spp. The affected patient should manifest relevant symptoms for at least 3 months. Chronic cavitary pulmonary aspergillosis is the most common subset of chronic pulmonary aspergillosis, which is often reported in patients previously treated for pulmonary tuberculosis, having residual cavities in their lungs. We present a case of a 55-year-old male patient treated for pulmonary tuberculosis 2 years back, now presenting with fever, shortness of breath, and hemoptysis with overt radiological changes from the baseline, positive direct microscopy, and serology for Aspergillus spp. and thus meeting the criteria for chronic cavitary pulmonary aspergillosis. Treatment with oral antifungal was initiated, but the follow-up data are unavailable due to patient noncompliance and lack of resources. We aim to emphasize the radiological and microbiological features of this condition to aid the early diagnosis and prompt treatment, as this may mimic similar pulmonary disorders and pose a significant challenge in the diagnosis and management outcomes.
RESUMO
Chromium (Cr) is a well-known environmental pollutant while less information is available on the role of Cr-resistant bacteria in the alleviation of Cr-stress in chili (Capsicum annum L.) plants. Effect of Cr-resistant bacterial strains on growth and Cr uptake by chili plants was investigated. The results revealed that Cr-stress showed a negative effect on germination, photosynthesis, and relative water content but the inoculation ameliorated the plant stress. Chromium-resistant bacterial strains enhanced the shoot and root growth (33% SL, 19.7% RL), shoot and root dry weight (35%, 32.9%), relative water content (32.25%), membrane stability index (46.52%) SPAD value (50.76%), Cr concentration in shoots and roots (19.87 and 18.52 mg kg-1), bioaccumulation and translocation factor (0.396 mgkg-1), and seedling vigor index (40.8%) of plants. Chromium-resistant bacterial strains enhanced the NPK uptake while reduced Cr uptake by plants. The morphological and biochemical examination of rhizobacterial strains (and NM28) resistant to Cr-stress revealed smooth, off-white colonies of bacteria composed of rod-shaped cells which are Gram positive in reaction while negative in catalase activity. High quantities of malic acid were produced by bacterial strains under study i.e. NM8 (926.12 µgmL-2) and NM28 (992.25 µgmL-2). These strains were identified as Bacillus cereus strain NM8 and Bacillus subtilis strain NM28 through 16S rRNA sequencing. Results showed that B. cereus strain NM28 is more effective than B. cereus strain NM8 in promoting the growth of Cr-stressed Chili that might be suitable to develop biofertilizer for sustainable production of vegetables under metal stress.
Assuntos
Capsicum , Cromo , Germinação , Capsicum/microbiologia , Capsicum/crescimento & desenvolvimento , Capsicum/metabolismo , Capsicum/efeitos dos fármacos , Cromo/metabolismo , Germinação/efeitos dos fármacos , Estresse Fisiológico/efeitos dos fármacos , Raízes de Plantas/microbiologia , Raízes de Plantas/metabolismo , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/efeitos dos fármacos , Bactérias/metabolismo , Bactérias/efeitos dos fármacos , Bactérias/genética , Bactérias/crescimento & desenvolvimento , Fotossíntese/efeitos dos fármacosRESUMO
Heavy metal accumulation increases rapidly in the environment due to anthropogenic activities and industrialization. The leather and surgical industry produces many contaminants containing heavy metals. Cadmium, a prominent contaminant, is linked to severe health risks, notably kidney and liver damage, especially among individuals exposed to contaminated wastewater. This study aims to leverage the natural cadmium resistance mechanisms in bacteria for bioaccumulation purposes. The industrial wastewater samples, characterized by an alarming cadmium concentration of 29.6 ppm, 52 ppm, and 76.4 ppm-far exceeding the recommended limit of 0.003 ppm-were subjected to screening for cadmium-resistant bacteria using cadmium-supplemented media with CdCl2. 16S rRNA characterization identified Vibrio cholerae and Proteus mirabilis as cadmium-resistant bacteria in the collected samples. Subsequently, the cadmium resistance-associated cadA gene was successfully amplified in Vibrio species and Proteus mirabilis, revealing a product size of 623 bp. Further analysis of the identified bacteria included the examination of virulent genes, specifically the tcpA gene (472 bp) associated with cholera and the UreC gene (317 bp) linked to urinary tract infections. To enhance the bioaccumulation of cadmium, the study proposes the potential suppression of virulent gene expression through in-silico gene-editing tools such as CRISPR-Cas9. A total of 27 gRNAs were generated for UreC, with five selected for expression. Similarly, 42 gRNA sequences were generated for tcpA, with eight chosen for expression analysis. The selected gRNAs were integrated into the lentiCRISPR v2 expression vector. This strategic approach aims to facilitate precise gene editing of disease-causing genes (tcpA and UreC) within the bacterial genome. In conclusion, this study underscores the potential utility of Vibrio species and Proteus mirabilis as effective candidates for the removal of cadmium from industrial wastewater, offering insights for future environmental remediation strategies.
Assuntos
Cólera , Infecções Urinárias , Vibrio , Humanos , Proteus mirabilis/genética , Cádmio/toxicidade , Sistemas CRISPR-Cas/genética , RNA Ribossômico 16S , Águas Residuárias , RNA Guia de Sistemas CRISPR-Cas , Vibrio/genéticaRESUMO
The present research examined patulin's presence across the whole supply chain of selected fruits. A comprehensive analysis was conducted on 442 samples of fruits (oranges, apples, apricots, lemons, and guava) to determine the presence of patulin contamination. This analysis used Liquid Chromatography (HPLC) with a UV detector. The findings indicate that 17, 23, and 28 % of selected fruit samples tested positive for patulin levels in farm, transportation, and market samples. However, the sample collected during the transportation step showed that 56 % (percentage of positive samples) of fruits have patulin levels greater than 50 µg/kg, and 41 % (percentage of positive samples) have greater levels than 50 µg/kg in market samples. The findings of the one-way analysis of variance indicated that no statistically significant variation existed between the amounts of patulin across the various stages of the food supply chain system (p > 0.05). Nevertheless, the analysis of the correlation study, namely Kendall's tau_b and Spearman's rho, denote a robust association between the levels of patulin and the food supply system. The apple samples exhibited the most significant average dietary intake of patulin, with an average value of 0.11 µg/kg bw/day. The maximum mean hazard quotient (HQ) of 0.28 was also recorded. The prevalence and incidence of patulin in specific fruits were found to be relatively high, and it was observed that market samples had elevated levels of patulin in the selected fruits.
RESUMO
Brassica napus (B. napus) is susceptible to multiple abiotic stresses that can affect plant growth and development, ultimately reducing crop yields. In the past, many genes that provide tolerance to abiotic stresses have been identified and characterized. Peroxidase (POD) proteins, members of the oxidoreductase enzyme family, play a critical role in protecting plants against abiotic stresses. This study demonstrated a comprehensive investigation of the POD gene family in B. napus. As a result, a total of 109 POD genes were identified across the 19 chromosomes and classified into five distinct subgroups. Further, 44 duplicate events were identified; of these, two gene pairs were tandem and 42 were segmental. Synteny analysis revealed that segmental duplication was more prominent than tandem duplication among POD genes. Expression pattern analysis based on the RNA-seq data of B. napus indicated that BnPOD genes were expressed differently in various tissues; most of them were expressed in roots rather than in other tissues. To validate these findings, we performed RT-qPCR analysis on ten genes; these genes showed various expression levels under abiotic stresses. Our findings not only furnish valuable insights into the evolutionary dynamics of the BnPOD gene family but also serve as a foundation for subsequent investigations into the functional roles of POD genes in B. napus.
RESUMO
Magnesium oxide nanoparticles (MgO NPs) have great potential to enhance the crop productivity and sustainability of agriculture. Still, a thorough understanding is lacking about its essentiality or toxicity and precise dose for the safe cultivation of oilseed crops. Thus, we assessed the dual effects of MgO NPs (control, 5, 10, 20, 40, 80, and 200 mg/L) on the seed germination, growth performance, photosynthesis, total soluble protein, total carbohydrates, oxidative stress markers (hydrogen peroxide as H2O2 and superoxide anion as O2â¢â), lipid peroxidation as MDA, and antioxidant defence machinery (SOD, CAT, APX, and GR activities, and GSH levels) of seven different oilseeds (Brassica napus L.) cultivars (ZY 758, ZD 649, ZD 635, ZD 619, GY 605, ZD 622, and ZD 630). Our findings revealed that low doses of MgO NPs (mainly at 10 mg/L) markedly boosted the seed germination, plant growth (shoot and root lengths) (15â22%), and biomass (fresh and dry) (11â19%) by improving the levels of photosynthetic pigments (14â27%), net photosynthetic rate, stomatal conductance, photosynthetic efficiency (Fv/Fm), total soluble protein and total carbohydrates (16â36%), antioxidant defence, and reducing the oxidative stress in B. napus tissues. Among all B. napus cultivars, these beneficial effects of MgO NPs were pronounced in ZD 635. ile, elevated levels of MgO NPs (particularly at 200 mg/L) induced oxidative stress, impaired antioxidant scavenging potential, photosynthetic inhibition, protein oxidation, and carbohydrate degradation and lead to inhibit the plant growth attributes. These inhibitory effects were more pronounced in ZD 622. Collectively, low-dose MgO NPs reinforced the Mg contents, protected the plant growth, photosynthesis, total soluble carbohydrates, enzyme activities, and minimized the oxidative stress. While, the excessive MgO NP levels impaired the above-reported traits. Overall, ZD 622 was highly susceptible to MgO NP toxicity and ZD 635 was found most tolerant to MgO NP toxicity.
Assuntos
Brassica napus , Nanopartículas , Antioxidantes/metabolismo , Óxido de Magnésio/farmacologia , Peróxido de Hidrogênio/metabolismo , Estresse Oxidativo , CarboidratosRESUMO
Fourier harmonic analysis (FHA) is a robust method for identification of minute changes in sperm nuclear shape that are indicative of reduced fertility. The current study was designed to develop a fertility prediction model for Nili-Ravi buffalo bulls through FHA of sperm. In experiment I, FHA technique was standardized, average sperm nuclear perimeter was measured and sperm nuclear shape plot of buffalo bull was constructed. Sperm of buffalo bulls (n = 10) were stained with YOYO-1 and Hoechst-33342 to differentiate live and dead, and digital images were captured using phase contrast and fluorescent microscopy. The images were analyzed by ImageJ software and 100 sperm/bull were evaluated. The results are described as mean ± SEM values of mean harmonic amplitude (mharm), skewness harmonic amplitude (skharm), kurtosis harmonic amplitude (kurharm) and variance harmonic amplitude (varharm) at Fourier frequencies 0-5 along with the cartesian and polar coordinate plots of buffalo bull sperm. In experiment II, a fertility prediction model was developed based on FHA of buffalo bull sperm. Semen samples of low (n = 6), medium (n = 3) and high (n = 8) fertility bulls were investigated for FHA of sperm and harmonic amplitudes (HA) were generated. Firstly, to determine if live and dead sperm population have unique nuclear shape distribution; the mean, skewness, kurtosis and variance HA 0-5 of 1700 live and 1294 dead spermatozoa of 17 bulls were evaluated. T-test signified a difference in the mharm0 (2.363 ± 0.01 vs. 2.439 ± 0.02), skharm0 (-0.0002 ± 0.07 vs. -0.266 ± 0.09), kurharm0 (-0.156 ± 0.07 vs. 0.260 ± 0.18), kurharm2 (0.142 ± 0.11 vs. 1.031 ± 0.32) and varharm4 (0.109 ± 0.00 vs. 0.082 ± 0.00) of live vs. dead sperm population (p < 0.05). Therefore, 100 live sperm/bull were further evaluated for mean, skewness, kurtosis and variance HA 0-5 values among high (n = 6) and low-fertility (n = 6) groups. Results of T-test showed higher values of mharm2 (0.739 ± 0.01 vs. 0.686 ± 0.00), mharm4 (0.105 ± 0.001 vs. 0.007 ± 0.001), and skharm0 (0.214 ± 0.109 vs. -0.244 ± 0.097) in high vs. low-fertility group (p < 0.05). In next step, five significantly different combinations of discriminant measures between high and low-fertility groups were obtained by discriminant analysis. In conclusion, mharm4, skharm0 and varharm2 correctly identified 91.7 % of bulls into their respective fertility groups, and upon cross validation the value of the canonical correlation was 0.928.
Assuntos
Búfalos , Fertilidade , Análise do Sêmen , Espermatozoides , Animais , Masculino , Búfalos/fisiologia , Espermatozoides/fisiologia , Fertilidade/fisiologia , Análise do Sêmen/veterinária , Análise do Sêmen/métodos , Análise de FourierRESUMO
BACKGROUND: Apnea-hypopnea index (AHI) and nadir oxygen saturation (SpO2) are the indexes used to measure the severity of obstructive sleep apnea (OSA). Obesity, measured by body mass index (BMI), is one of the main contributing factors to the onset and severity of OSA in patients. This study was conducted to find the association between BMI and OSA severity indexes, mainly AHI and nadir SpO2 levels. METHODS: Polysomnography reports of patients with diagnosed OSA in a teaching hospital were retrospectively reviewed. BMI, AHI, and nadir SpO2 levels were recorded from the sleep study reports of the patients. Spearman's Rho test was applied to find the correlation between BMI and AHI/nadir Spo2 levels. RESULTS: A total of 167 patients were included in the study, comprising 83 males and 84 females. The Mann-Whitney U test was utilized to investigate the association between BMI and gender and age groups. The analysis revealed a significant difference in BMI between males and females, with females having a higher BMI. However, there was no significant difference in BMI among individuals in the early middle and late middle age groups. Spearman's Rho test was employed to explore the correlation between BMI and AHI/nadir SpO2 levels. The results indicated no significant correlation between BMI and AHI (p = .122) or nadir SpO2 levels (p = .239). CONCLUSION: Contrary to common belief, BMI was not linked to the severity of OSA. It implies that several other factors, independent of BMI, play a role in the disease progression and severity.
RESUMO
Soy protein is a promising nutritional source with improved functionality and bioactivities due to conjugation with polyphenols (PP)-the conjugates between soy protein and PP held by covalent and noncovalent bonds. Different approaches, including thermodynamics, spectroscopy, and molecular docking simulations, can demonstrate the outcomes and mechanism of these conjugates. The soy protein, PP structure, matrix properties (temperature, pH), and interaction mechanism alter the ζ-potential, secondary structure, thermal stability, and surface hydrophobicity of proteins and also improve the techno-functional properties such as gelling ability, solubility, emulsifying, and foaming properties. Soy protein-PP conjugates also reveal enhanced in vitro digestibility, anti-allergic, antioxidant, anticancer, anti-inflammatory, and antimicrobial activities. Thus, these conjugates may be employed as edible film additives, antioxidant emulsifiers, hydrogels, and nanoparticles in the food industry. Future research is needed to specify the structure-function associations of soy protein-PP conjugates that may affect their functionality and application in the food industry.