RESUMO
Oenopia sauzeti Mulsant are predators of various aphids, such as Myzus persicae, Rhopalosiphum maidis, and Sitobion avenae. We sequenced the mitochondrial genome (mitogenome) of O. sauzeti. Consistent with other mitogenomes of Coleoptera, the circular mitogenome of O. sauzeti consists of 17,630 bp, including 13 PCG genes, 2 rRNA genes, and 22 tRNA genes. The mitogenome differs in 2 long non-coding regions of 1285 bp and 1843 bp. The 22 tRNA genes products are folded into the typical cloverleaf secondary structure, except trnS1, which lacks the dihydrouridine (DHU) arm. The base composition is AT-biased (80.1%). The phylogenetic tree confirms monophyly at the genus level within the Coccinellinae and supports O. sauzeti as a sister group to Aiolocaria hexaspilota.
RESUMO
We sequenced and annotated mitochondrial genome (mitogenome) of oilseed rape pest Psylliodes punctifrons Baly for the first time. The mitogenome is 15,611 bp, and the nucleotide composition of 37 genes is highly A + T biased (A: 34.2, C: 11.6, G: 12.1, and T:42.1). All PCGs start with ATN and stop with TAR, except COX3 and ND4 that stop with incomplete codon T-. The phylogenetic tree confirms that P. punctifrons is clustered with other Psylliodes species. This study enriches the mitogenomes of agricultural pests.
RESUMO
OBJECTIVE: To explore the expression of tryptase and chymase in human lung tissue of anaphylactic shock and its value for forensic medicine. METHODS: With ten carbon monoxide poisoning cases as control group, the levels of tryptase and chymase were observed by immunofluorescence and analyzed using the Image Analyze and the Image-pro plus 5.0.2. The positive mast cells were counted and the levels of the tryptase and chymase were calculated respectively. RESULTS: There was a statistically significant difference (P < 0.05) for the tryptase and chymase concentrations in the lung tissue between the anaphylactic shock group and the control group. CONCLUSION: The levels of the tryptase and the chymase expression are greatly increased in human lung tissue of anaphylactic shock, which may provide the morphological evidence and reference for the diagnosis of anaphylactic shock in forensic practice.