Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 54
Filtrar
1.
Nucleic Acids Res ; 2024 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-38953172

RESUMO

Zinc finger (ZnF) domains appear in a pool of structural contexts and despite their small size achieve varying target specificities, covering single-stranded and double-stranded DNA and RNA as well as proteins. Combined with other RNA-binding domains, ZnFs enhance affinity and specificity of RNA-binding proteins (RBPs). The ZnF-containing immunoregulatory RBP Roquin initiates mRNA decay, thereby controlling the adaptive immune system. Its unique ROQ domain shape-specifically recognizes stem-looped cis-elements in mRNA 3'-untranslated regions (UTR). The N-terminus of Roquin contains a RING domain for protein-protein interactions and a ZnF, which was suggested to play an essential role in RNA decay by Roquin. The ZnF domain boundaries, its RNA motif preference and its interplay with the ROQ domain have remained elusive, also driven by the lack of high-resolution data of the challenging protein. We provide the solution structure of the Roquin-1 ZnF and use an RBNS-NMR pipeline to show that the ZnF recognizes AU-rich RNAs. We systematically refine the contributions of adenines in a poly(U)-background to specific complex formation. With the simultaneous binding of ROQ and ZnF to a natural target transcript of Roquin, our study for the first time suggests how Roquin integrates RNA shape and sequence features through the ROQ-ZnF tandem.

2.
Nucleic Acids Res ; 52(11): 6687-6706, 2024 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-38783391

RESUMO

The single-stranded RNA genome of SARS-CoV-2 is highly structured. Numerous helical stem-loop structures interrupted by mismatch motifs are present in the functionally important 5'- and 3'-UTRs. These mismatches modulate local helical geometries and feature unusual arrays of hydrogen bonding donor and acceptor groups. However, their conformational and dynamical properties cannot be directly inferred from chemical probing and are difficult to predict theoretically. A mismatch motif (SL1-motif) consisting of three consecutive U•U base pairs is located in stem-loop 1 of the 3'-UTR. We combined NMR-spectroscopy and MD-simulations to investigate its structure and dynamics. All three U•U base pairs feature two direct hydrogen bonds and are as stable as Watson-Crick A:U base pairs. Plasmodium falciparum 25S rRNA contains a triple U•U mismatch motif (Pf-motif) differing from SL1-motif only with respect to the orientation of the two closing base pairs. Interestingly, while the geometry of the outer two U•U mismatches was identical in both motifs the preferred orientation of the central U•U mismatch was different. MD simulations and potassium ion titrations revealed that the potassium ion-binding mode to the major groove is connected to the different preferred geometries of the central base pair in the two motifs.


Assuntos
Regiões 3' não Traduzidas , Pareamento Incorreto de Bases , Motivos de Nucleotídeos , RNA Viral , SARS-CoV-2 , Humanos , Pareamento de Bases , COVID-19/virologia , Genoma Viral , Ligação de Hidrogênio , Simulação de Dinâmica Molecular , Conformação de Ácido Nucleico , Plasmodium falciparum/genética , RNA Viral/química , RNA Viral/genética , SARS-CoV-2/genética , SARS-CoV-2/química
3.
J Biol Chem ; 300(7): 107457, 2024 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-38866324

RESUMO

AT-rich interacting domain (ARID)-containing proteins, Arids, are a heterogeneous DNA-binding protein family involved in transcription regulation and chromatin processing. For the member Arid5a, no exact DNA-binding preference has been experimentally defined so far. Additionally, the protein binds to mRNA motifs for transcript stabilization, supposedly through the DNA-binding ARID domain. To date, however, no unbiased RNA motif definition and clear dissection of nucleic acid-binding through the ARID domain have been undertaken. Using NMR-centered biochemistry, we here define the Arid5a DNA preference. Further, high-throughput in vitro binding reveals a consensus RNA-binding motif engaged by the core ARID domain. Finally, transcriptome-wide binding (iCLIP2) reveals that Arid5a has a weak preference for (A)U-rich regions in pre-mRNA transcripts of factors related to RNA processing. We find that the intrinsically disordered regions flanking the ARID domain modulate the specificity and affinity of DNA binding, while they appear crucial for RNA interactions. Ultimately, our data suggest that Arid5a uses its extended ARID domain for bifunctional gene regulation and that the involvement of IDR extensions is a more general feature of Arids in interacting with different nucleic acids at the chromatin-mRNA interface.

4.
Nucleic Acids Res ; 51(20): 11318-11331, 2023 11 10.
Artigo em Inglês | MEDLINE | ID: mdl-37791874

RESUMO

We present the high-resolution structure of stem-loop 4 of the 5'-untranslated region (5_SL4) of the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) genome solved by solution state nuclear magnetic resonance spectroscopy. 5_SL4 adopts an extended rod-like structure with a single flexible looped-out nucleotide and two mixed tandem mismatches, each composed of a G•U wobble base pair and a pyrimidine•pyrimidine mismatch, which are incorporated into the stem-loop structure. Both the tandem mismatches and the looped-out residue destabilize the stem-loop structure locally. Their distribution along the 5_SL4 stem-loop suggests a role of these non-canonical elements in retaining functionally important structural plasticity in particular with regard to the accessibility of the start codon of an upstream open reading frame located in the RNA's apical loop. The apical loop-although mostly flexible-harbors residual structural features suggesting an additional role in molecular recognition processes. 5_SL4 is highly conserved among the different variants of SARS-CoV-2 and can be targeted by small molecule ligands, which it binds with intermediate affinity in the vicinity of the non-canonical elements within the stem-loop structure.


Assuntos
COVID-19 , SARS-CoV-2 , Humanos , Sequência de Bases , COVID-19/virologia , Espectroscopia de Ressonância Magnética , Conformação de Ácido Nucleico , RNA Viral/química , SARS-CoV-2/química , SARS-CoV-2/genética
5.
Biol Chem ; 405(4): 229-239, 2024 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-37942876

RESUMO

HnRNPs are ubiquitously expressed RNA-binding proteins, tightly controlling posttranscriptional gene regulation. Consequently, hnRNP networks are essential for cellular homeostasis and their dysregulation is associated with cancer and other diseases. However, the physiological function of hnRNPs in non-cancerous cell systems are poorly understood. We analyzed the importance of HNRNPDL in endothelial cell functions. Knockdown of HNRNPDL led to impaired proliferation, migration and sprouting of spheroids. Transcriptome analysis identified cyclin D1 (CCND1) and tropomyosin 4 (TPM4) as targets of HNRNPDL, reflecting the phenotypic changes after knockdown. Our findings underline the importance of HNRNPDL for the homeostasis of physiological processes in endothelial cells.


Assuntos
Células Endoteliais , Ribonucleoproteínas Nucleares Heterogêneas , Ribonucleoproteínas Nucleares Heterogêneas/genética , Células Endoteliais/metabolismo , Proteínas de Ligação a RNA/metabolismo
6.
Angew Chem Int Ed Engl ; 62(14): e202217171, 2023 03 27.
Artigo em Inglês | MEDLINE | ID: mdl-36748955

RESUMO

The outbreak of COVID-19 in December 2019 required the formation of international consortia for a coordinated scientific effort to understand and combat the virus. In this Viewpoint Article, we discuss how the NMR community has gathered to investigate the genome and proteome of SARS-CoV-2 and tested them for binding to low-molecular-weight binders. External factors including extended lockdowns due to the global pandemic character of the viral infection triggered the transition from locally focused collaborative research conducted within individual research groups to digital exchange formats for immediate discussion of unpublished results and data analysis, sample sharing, and coordinated research between more than 50 groups from 18 countries simultaneously. We discuss key lessons that might pertain after the end of the pandemic and challenges that we need to address.


Assuntos
COVID-19 , Humanos , SARS-CoV-2 , Controle de Doenças Transmissíveis , Espectroscopia de Ressonância Magnética , Imageamento por Ressonância Magnética
7.
RNA ; 26(5): 648-663, 2020 05.
Artigo em Inglês | MEDLINE | ID: mdl-32127384

RESUMO

Hypoxia is a hallmark of solid cancers, supporting proliferation, angiogenesis, and escape from apoptosis. There is still limited understanding of how cancer cells adapt to hypoxic conditions and survive. We analyzed transcriptome changes of human lung and breast cancer cells under chronic hypoxia. Hypoxia induced highly concordant changes in transcript abundance, but divergent splicing responses, underlining the cell type-specificity of alternative splicing programs. While RNA-binding proteins were predominantly reduced, hypoxia specifically induced muscleblind-like protein 2 (MBNL2). Strikingly, MBNL2 induction was critical for hypoxia adaptation by controlling the transcript abundance of hypoxia response genes, such as vascular endothelial growth factor A (VEGFA) MBNL2 depletion reduced the proliferation and migration of cancer cells, demonstrating an important role of MBNL2 as cancer driver. Hypoxia control is specific for MBNL2 and not shared by its paralog MBNL1. Thus, our study revealed MBNL2 as central mediator of cancer cell responses to hypoxia, regulating the expression and alternative splicing of hypoxia-induced genes.


Assuntos
Neoplasias da Mama/genética , Neoplasias Pulmonares/genética , Proteínas de Ligação a RNA/genética , Hipóxia Tumoral/genética , Fator A de Crescimento do Endotélio Vascular/genética , Neoplasias da Mama/metabolismo , Neoplasias da Mama/patologia , Linhagem Celular Tumoral , Proliferação de Células/genética , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/patologia , Transcriptoma/genética
8.
Nucleic Acids Res ; 48(13): 7385-7403, 2020 07 27.
Artigo em Inglês | MEDLINE | ID: mdl-32491174

RESUMO

Adenylate/uridylate-rich elements (AREs) are the most common cis-regulatory elements in the 3'-untranslated region (UTR) of mRNAs, where they fine-tune turnover by mediating mRNA decay. They increase plasticity and efficacy of mRNA regulation and are recognized by several ARE-specific RNA-binding proteins (RBPs). Typically, AREs are short linear motifs with a high content of complementary A and U nucleotides and often occur in multiple copies. Although thermodynamically rather unstable, the high AU-content might enable transient secondary structure formation and modify mRNA regulation by RBPs. We have recently suggested that the immunoregulatory RBP Roquin recognizes folded AREs as constitutive decay elements (CDEs), resulting in shape-specific ARE-mediated mRNA degradation. However, the structural evidence for a CDE-like recognition of AREs by Roquin is still lacking. We here present structures of CDE-like folded AREs, both in their free and protein-bound form. Moreover, the AREs in the UCP3 3'-UTR are additionally bound by the canonical ARE-binding protein AUF1 in their linear form, adopting an alternative binding-interface compared to the recognition of their CDE structure by Roquin. Strikingly, our findings thus suggest that AREs can be recognized in multiple ways, allowing control over mRNA regulation by adapting distinct conformational states, thus providing differential accessibility to regulatory RBPs.


Assuntos
Elementos Ricos em Adenilato e Uridilato , Proteínas de Ligação a RNA/química , Ubiquitina-Proteína Ligases/química , Sítios de Ligação , Células HEK293 , Humanos , Simulação de Acoplamento Molecular , Motivos de Nucleotídeos , Ligação Proteica , Proteínas de Ligação a RNA/metabolismo , Ubiquitina-Proteína Ligases/metabolismo
9.
Nucleic Acids Res ; 48(22): 12415-12435, 2020 12 16.
Artigo em Inglês | MEDLINE | ID: mdl-33167030

RESUMO

The current pandemic situation caused by the Betacoronavirus SARS-CoV-2 (SCoV2) highlights the need for coordinated research to combat COVID-19. A particularly important aspect is the development of medication. In addition to viral proteins, structured RNA elements represent a potent alternative as drug targets. The search for drugs that target RNA requires their high-resolution structural characterization. Using nuclear magnetic resonance (NMR) spectroscopy, a worldwide consortium of NMR researchers aims to characterize potential RNA drug targets of SCoV2. Here, we report the characterization of 15 conserved RNA elements located at the 5' end, the ribosomal frameshift segment and the 3'-untranslated region (3'-UTR) of the SCoV2 genome, their large-scale production and NMR-based secondary structure determination. The NMR data are corroborated with secondary structure probing by DMS footprinting experiments. The close agreement of NMR secondary structure determination of isolated RNA elements with DMS footprinting and NMR performed on larger RNA regions shows that the secondary structure elements fold independently. The NMR data reported here provide the basis for NMR investigations of RNA function, RNA interactions with viral and host proteins and screening campaigns to identify potential RNA binders for pharmaceutical intervention.


Assuntos
COVID-19/prevenção & controle , Espectroscopia de Ressonância Magnética/métodos , Conformação de Ácido Nucleico , RNA Viral/química , SARS-CoV-2/genética , Regiões 3' não Traduzidas/genética , Sequência de Bases , COVID-19/epidemiologia , COVID-19/virologia , Mudança da Fase de Leitura do Gene Ribossômico/genética , Genoma Viral/genética , Humanos , Modelos Moleculares , Pandemias , SARS-CoV-2/fisiologia
10.
RNA ; 25(1): 158-167, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30337459

RESUMO

The tetracycline-binding RNA aptamer (TC-aptamer) is a synthetic riboswitch that binds the antibiotic tetracycline (TC) with exceptionally high affinity. Although a crystal structure exists of the TC-bound state, little is known about the conformational dynamics and changes upon ligand binding. In this study, pulsed electron paramagnetic resonance techniques for measuring distances (PELDOR) in combination with rigid nitroxide spin labels (Çm spin label) were used to investigate the conformational flexibility of the TC-aptamer in the presence and absence of TC at different Mg2+ concentrations. TC was found to be the essential factor for stabilizing the tertiary structure at intermediate Mg2+ concentrations. At higher Mg2+ concentrations, Mg2+ alone is sufficient to stabilize the tertiary structure. In addition, the orientation of the two spin-labeled RNA helices with respect to each other was analyzed with orientation-selective PELDOR and compared to the crystal structure. These results demonstrate for the first time the unique value of the Çm spin label in combination with PELDOR to provide information about conformational flexibilities and orientations of secondary structure elements of biologically relevant RNAs.


Assuntos
Aptâmeros de Nucleotídeos/química , Aptâmeros de Nucleotídeos/metabolismo , Magnésio/química , Riboswitch , Tetraciclina/metabolismo , Sequência de Bases , Cristalografia por Raios X , Espectroscopia de Ressonância de Spin Eletrônica , Modelos Moleculares , Conformação de Ácido Nucleico , Estabilidade de RNA , Marcadores de Spin
11.
Nucleic Acids Res ; 47(1): 15-28, 2019 01 10.
Artigo em Inglês | MEDLINE | ID: mdl-30462266

RESUMO

The ability of the cytidine analog Çmf to act as a position specific reporter of RNA-dynamics was spectroscopically evaluated. Çmf-labeled single- and double-stranded RNAs differ in their fluorescence lifetimes, quantum yields and anisotropies. These observables were also influenced by the nucleobases flanking Çmf. This conformation and position specificity allowed to investigate the binding dynamics and mechanism of neomycin to its aptamer N1 by independently incorporating Çmf at four different positions within the aptamer. Remarkably fast binding kinetics of neomycin binding was observed with stopped-flow measurements, which could be satisfactorily explained with a two-step binding. Conformational selection was identified as the dominant mechanism.


Assuntos
Aptâmeros de Nucleotídeos/química , Neomicina/química , RNA de Cadeia Dupla/química , Aptâmeros de Nucleotídeos/genética , Sítios de Ligação/genética , Citidina/análogos & derivados , Fluorescência , Cinética , RNA de Cadeia Dupla/isolamento & purificação , Espectrometria de Fluorescência , Coloração e Rotulagem/métodos
12.
Angew Chem Int Ed Engl ; 60(35): 19191-19200, 2021 08 23.
Artigo em Inglês | MEDLINE | ID: mdl-34161644

RESUMO

SARS-CoV-2 contains a positive single-stranded RNA genome of approximately 30 000 nucleotides. Within this genome, 15 RNA elements were identified as conserved between SARS-CoV and SARS-CoV-2. By nuclear magnetic resonance (NMR) spectroscopy, we previously determined that these elements fold independently, in line with data from in vivo and ex-vivo structural probing experiments. These elements contain non-base-paired regions that potentially harbor ligand-binding pockets. Here, we performed an NMR-based screening of a poised fragment library of 768 compounds for binding to these RNAs, employing three different 1 H-based 1D NMR binding assays. The screening identified common as well as RNA-element specific hits. The results allow selection of the most promising of the 15 RNA elements as putative drug targets. Based on the identified hits, we derive key functional units and groups in ligands for effective targeting of the RNA of SARS-CoV-2.


Assuntos
Genoma , RNA Viral/metabolismo , SARS-CoV-2/genética , Bibliotecas de Moléculas Pequenas/metabolismo , Avaliação Pré-Clínica de Medicamentos , Ligantes , Estrutura Molecular , Conformação de Ácido Nucleico , Espectroscopia de Prótons por Ressonância Magnética , RNA Viral/química , Bibliotecas de Moléculas Pequenas/química
13.
Biospektrum (Heidelb) ; 27(4): 351-354, 2021.
Artigo em Alemão | MEDLINE | ID: mdl-34219980

RESUMO

Posttranscriptional regulation at mRNA level is defined not only by sequence, but also by the structure of the mRNA. Structures in the untranslated regions control stability, translation and localization of an mRNA. Evolutionary conservation can be used to identify such regulatory active structures in mRNAs. For example, constitutive decay element (CDE) stem-loops are recognized by Roquin proteins in an exclusively shape-specific manner. Strikingly, some CDEs can serve a dual function in gene repression depending on their folding status.

14.
RNA ; 24(3): 324-331, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29263134

RESUMO

HnRNP D, better known as AUF1, is an extensively studied protein that controls a variety of cellular pathways. Consequently, its expression has to be tightly regulated to prevent the onset of pathologies. In contrast, the cellular functions and regulation of its ubiquitously expressed paralog hnRNP DL are barely explored. Here, we present an intricate crosstalk between these two proteins. Both hnRNP D and DL are able to control their own expression by alternative splicing of cassette exons in their 3'UTRs. Exon inclusion produces mRNAs degraded by nonsense-mediated decay. Moreover, hnRNP D and DL control the expression of one another by the same mechanism. Thus, we identified two novel ways of how hnRNP D expression is controlled. The tight interconnection of expression control directly links hnRNP DL to hnRNP D-related diseases and emphasizes the importance of a systematic analysis of its cellular functions.


Assuntos
Processamento Alternativo , Regulação da Expressão Gênica/genética , Ribonucleoproteínas Nucleares Heterogêneas Grupo D/genética , Ribonucleoproteínas Nucleares Heterogêneas/genética , Degradação do RNAm Mediada por Códon sem Sentido/genética , Ribonucleoproteínas/genética , Regiões 3' não Traduzidas/genética , Éxons , Genes Reporter , Células HeLa , Ribonucleoproteína Nuclear Heterogênea D0 , Ribonucleoproteínas Nucleares Heterogêneas Grupo D/fisiologia , Ribonucleoproteínas Nucleares Heterogêneas Grupo L/genética , Homeostase , Humanos , RNA Mensageiro/genética , Ribonucleoproteínas/fisiologia
15.
Nucleic Acids Res ; 46(8): e48, 2018 05 04.
Artigo em Inglês | MEDLINE | ID: mdl-29420816

RESUMO

Splicing is an essential and highly regulated process in mammalian cells. We developed a synthetic riboswitch that efficiently controls alternative splicing of a cassette exon in response to the small molecule ligand tetracycline. The riboswitch was designed to control the accessibility of the 3' splice site by placing the latter inside the closing stem of a conformationally controlled tetracycline aptamer. In the presence of tetracycline, the cassette exon is skipped, whereas it is included in the ligand's absence. The design allows for an easy, context-independent integration of the regulatory device into any gene of interest. Portability of the device was shown through its functionality in four different systems: a synthetic minigene, a reporter gene and two endogenous genes. Furthermore, riboswitch functionality to control cellular signaling cascades was demonstrated by using it to specifically induce cell death through the conditionally controlled expression of CD20, which is a target in cancer therapy.


Assuntos
Processamento Alternativo , Éxons , Riboswitch , Processamento Alternativo/efeitos dos fármacos , Animais , Antígenos CD20/genética , Aptâmeros de Nucleotídeos/química , Aptâmeros de Nucleotídeos/genética , Sequência de Bases , Morte Celular/genética , Células HEK293 , Células HeLa , Humanos , Células MCF-7 , Conformação de Ácido Nucleico , Sítios de Splice de RNA , Estabilidade de RNA , Riboswitch/efeitos dos fármacos , Riboswitch/genética , Biologia Sintética , Tetraciclina/farmacologia
16.
Nucleic Acids Res ; 46(22): 12109-12125, 2018 12 14.
Artigo em Inglês | MEDLINE | ID: mdl-30295819

RESUMO

Post-transcriptional gene regulation controls the amount of protein produced from a specific mRNA by altering both its decay and translation rates. Such regulation is primarily achieved by the interaction of trans-acting factors with cis-regulatory elements in the untranslated regions (UTRs) of mRNAs. These interactions are guided either by sequence- or structure-based recognition. Similar to sequence conservation, the evolutionary conservation of a UTR's structure thus reflects its functional importance. We used such structural conservation to identify previously unknown cis-regulatory elements. Using the RNA folding program Dynalign, we scanned all UTRs of humans and mice for conserved structures. Characterizing a subset of putative conserved structures revealed a binding site of the RNA-binding protein Roquin. Detailed functional characterization in vivo enabled us to redefine the binding preferences of Roquin and identify new target genes. Many of these new targets are unrelated to the established role of Roquin in inflammation and immune responses and thus highlight additional, unstudied cellular functions of this important repressor. Moreover, the expression of several Roquin targets is highly cell-type-specific. In consequence, these targets are difficult to detect using methods dependent on mRNA abundance, yet easily detectable with our unbiased strategy.


Assuntos
Biologia Computacional/métodos , Regulação da Expressão Gênica , Dobramento de RNA , Proteínas de Ligação a RNA/química , Ubiquitina-Proteína Ligases/química , Regiões 3' não Traduzidas , Animais , Sítios de Ligação , Linhagem Celular , Simulação por Computador , Análise Mutacional de DNA , Células HEK293 , Células HeLa , Humanos , Camundongos , Conformação de Ácido Nucleico , Nucleotídeos/genética , Ligação Proteica , RNA Mensageiro/metabolismo , Proteínas de Ligação a RNA/genética , Transcrição Gênica , Ubiquitina-Proteína Ligases/genética
17.
Nucleic Acids Res ; 45(22): e181, 2017 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-29036355

RESUMO

Synthetic RNA-based systems have increasingly been used for the regulation of eukaryotic gene expression. Due to their structural properties, riboregulators provide a convenient basis for the development of ligand-dependent controllable systems. Here, we demonstrate reversible conditional control of miRNA biogenesis with an aptamer domain as a sensing unit connected to a natural miRNA precursor for the first time. For the design of the pre-miR switch, we replaced the natural terminal loop with the TetR aptamer. Thus, the TetR aptamer was positioned close to the Dicer cleavage sites, which allowed sterical control over pre-miR processing by Dicer. Our design proved to be highly versatile, allowing us to regulate the biogenesis of three structurally different miRNAs: miR-126, -34a and -199a. Dicer cleavage was inhibited up to 143-fold via co-expression of the TetR protein, yet could be completely restored upon addition of doxycycline. Moreover, we showed the functionality of the pre-miR switches for gene regulation through the interaction of the respective miRNA with its specific target sequence. Our designed device is capable of robust and reversible control of miRNA abundance. Thus, we offer a novel investigational tool for functional miRNA analysis.


Assuntos
Regulação da Expressão Gênica , Mamíferos/genética , MicroRNAs/genética , Precursores de RNA/genética , Animais , Aptâmeros de Nucleotídeos/genética , Aptâmeros de Nucleotídeos/metabolismo , Sequência de Bases , RNA Helicases DEAD-box/metabolismo , Células HeLa , Humanos , Mamíferos/metabolismo , MicroRNAs/biossíntese , Modelos Genéticos , Proteínas Repressoras/genética , Proteínas Repressoras/metabolismo , Ribonuclease III/metabolismo
19.
Biochim Biophys Acta ; 1859(7): 848-59, 2016 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-27168114

RESUMO

Translation is an energy-intensive process and tightly regulated. Generally, translation is initiated in a cap-dependent manner. Under stress conditions, typically found within the tumor microenvironment in association with e.g. nutrient deprivation or hypoxia, cap-dependent translation decreases, and alternative modes of translation initiation become more important. Specifically, internal ribosome entry sites (IRES) facilitate translation of specific mRNAs under otherwise translation-inhibitory conditions. This mechanism is controlled by IRES trans-acting factors (ITAF), i.e. by RNA-binding proteins, which interact with and determine the activity of selected IRESs. We aimed at characterizing the translational regulation of the IL-33 decoy receptor sST2, which was enhanced by fibroblast growth factor 2 (FGF2). We identified and verified an IRES within the 5'UTR of sST2. Furthermore, we found that MEK/ERK signaling contributes to FGF2-induced, sST2-IRES activation and translation. Determination of the sST2-5'UTR structure by in-line probing followed by deletion analyses identified 23 nucleotides within the sST2-5'UTR to be required for optimal IRES activity. Finally, we show that the RNA-binding protein heterogeneous ribonucleoprotein A1 (hnRNP A1) binds to the sST2-5'UTR, acts as an ITAF, and thus controls the activity of the sST2-IRES and consequently sST2 translation. Specifically, FGF2 enhances nuclear-cytoplasmic translocation of hnRNP A1, which requires intact MEK/ERK activity. In summary, we provide evidence that the sST2-5'UTR contains an IRES element, which is activated by a MEK/ERK-dependent increase in cytoplasmic localization of hnRNP A1 in response to FGF2, enhancing the translation of sST2.


Assuntos
Fator 2 de Crescimento de Fibroblastos/farmacologia , Ribonucleoproteínas Nucleares Heterogêneas Grupo A-B/fisiologia , Sítios Internos de Entrada Ribossomal/fisiologia , Biossíntese de Proteínas , Receptores de Superfície Celular/genética , Receptores de Superfície Celular/metabolismo , Regiões 5' não Traduzidas/efeitos dos fármacos , Sítios de Ligação/efeitos dos fármacos , Regulação da Expressão Gênica/efeitos dos fármacos , Ribonucleoproteína Nuclear Heterogênea A1 , Humanos , Proteína 1 Semelhante a Receptor de Interleucina-1 , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Sistema de Sinalização das MAP Quinases/genética , Células MCF-7 , Biossíntese de Proteínas/efeitos dos fármacos , Biossíntese de Proteínas/genética , Solubilidade
20.
RNA ; 20(8): 1163-72, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24951555

RESUMO

The U-turn is a classical three-dimensional RNA folding motif first identified in the anticodon and T-loops of tRNAs. It also occurs frequently as a building block in other functional RNA structures in many different sequence and structural contexts. U-turns induce sharp changes in the direction of the RNA backbone and often conform to the 3-nt consensus sequence 5'-UNR-3' (N = any nucleotide, R = purine). The canonical U-turn motif is stabilized by a hydrogen bond between the N3 imino group of the U residue and the 3' phosphate group of the R residue as well as a hydrogen bond between the 2'-hydroxyl group of the uridine and the N7 nitrogen of the R residue. Here, we demonstrate that a protonated cytidine can functionally and structurally replace the uridine at the first position of the canonical U-turn motif in the apical loop of the neomycin riboswitch. Using NMR spectroscopy, we directly show that the N3 imino group of the protonated cytidine forms a hydrogen bond with the backbone phosphate 3' from the third nucleotide of the U-turn analogously to the imino group of the uridine in the canonical motif. In addition, we compare the stability of the hydrogen bonds in the mutant U-turn motif to the wild type and describe the NMR signature of the C+-phosphate interaction. Our results have implications for the prediction of RNA structural motifs and suggest simple approaches for the experimental identification of hydrogen bonds between protonated C-imino groups and the phosphate backbone.


Assuntos
Citidina , Conformação de Ácido Nucleico , Motivos de Nucleotídeos , RNA/química , Sequência de Bases , Ligação de Hidrogênio , Modelos Moleculares , Ressonância Magnética Nuclear Biomolecular , Fosfatos/química , RNA/genética , Riboswitch , Uridina
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA