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1.
J Strength Cond Res ; 37(2): 265-269, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36026487

RESUMO

ABSTRACT: Christian, JR, Gothart, SE, Graham, HK, Barganier, KD, and Whitehead, PN. Analysis of the activation of upper-extremity muscles during various chest press modalities. J Strength Cond Res 37(2): 265-269, 2023-The most effective way to increase muscular strength is through the implementation of resistance training programs. The purpose of this study was to determine the level of muscle activation of the anterior deltoid (AD), medial deltoid (MD), and pectoralis major (PM) during various bench press scenarios. Twenty subjects (10 male and 10 female subjects; age, 24.0 ± 3.0 years) with a minimum of 1 year of resistance training were recruited to participate. Six surface electromyographical sensors were placed bilaterally on the targeted muscles. Subjects performed a 1 repetition maximum (1RM) of dumbbell (DB) and barbell (BB) flat bench press across 2 sessions followed by 6 repetitions at an incline, flat, and decline position at a submaximal 70% 1RM BB and DB. Mean peak values for muscle activation were analyzed for each variation. A repeated-measures 1-way analysis of variance was used to compare muscle activation patterns across conditions. Significance was set at p ≤ 0.05. Activation of AD was significantly higher during incline BB compared with decline BB and decline DB ( p ≤ 0.019). Activation of MD was significantly lower than that of AD across all modalities ( p ≤ 0.040). Activation of PM was significantly lower during incline DB compared with decline DB ( p = 0.011). Differences among AD and PM may have been the result of the stability stipulation variations between DB or BB and the large biacromial breadth requirement. Lack of differentiation of muscle fibers within the PM during sensor placement could have led to lesser muscle activation recordings during decline. As significance was evident for PM when comparing genders ( p ≤ 0.021), and no significance was found among AD or MD, it can be inferred that the larger amounts of adipose tissue in the female subjects caused irregularities in the PM data.


Assuntos
Músculo Esquelético , Treinamento Resistido , Humanos , Masculino , Feminino , Adulto Jovem , Adulto , Músculo Esquelético/fisiologia , Eletromiografia , Músculos Peitorais/fisiologia , Extremidade Superior , Força Muscular/fisiologia , Levantamento de Peso/fisiologia
2.
Environ Monit Assess ; 195(10): 1188, 2023 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-37698767

RESUMO

In the Awash River basin (Ethiopia), massive urbanization and industrialization, driven by rapid development and human settlement, are detrimental to the environment and human health as pollutants such as heavy metals (HMs) find their way into water bodies without proper treatment. The purpose of this study was to assess the HMs content and pollution sources within the basin. In this context, a total of 205 samples were collected from 21 surface water sampling stations. Heavy metal concentrations were measured using the Perkin Elmer NexION 350 ICP-MS with inductively coupled plasma. Findings demonstrate that high levels of HMs, such as Al, Mn, Mo, As, V, Fe, and Ba were exhibited with the value of 1257 µg/L, 626.8 µg/L, 116.7 µg/L, 61.2 µg/L, 100.5 µg/L, 1082.7 µg/L, and 211.7 µg/L, respectively. Among 20 HMs analyzed, 20% of the parameters within the study area were above the WHO limit for drinking water; Al (157 µg/L), V (100.5 µg/L), Fe (1082.7 µg/L), Mn (626.8 µg/L), and Mo (103.8 µg/L) were exhibited at sites along the river system. Likewise, 57% of water samples showed high values of As at many stations down the river systems. In particular, high HM concentrations seen in the upper Awash are primarily controlled by anthropogenic activities such as untreated industrial, agricultural, and domestic discharges, while the high HM concentrations in the middle Awash samples were likely due to the influence from the Lake Beseka that has high HM concentrations due to geological process. In conclusion, securing potable water for the rapidly increasing population in Addis Ababa and in the watersheds of Awash is unsafe to sustain the environment and the human health.


Assuntos
Arsênio , Metais Pesados , Humanos , Molibdênio , Efeitos Antropogênicos , Lagos , Rios , Etiópia , Monitoramento Ambiental , Água
3.
J Strength Cond Res ; 36(12): 3468-3472, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-32881841

RESUMO

ABSTRACT: Conners, RT, Whitehead, PN, Dodds, FT, Schott, KD, and Quick, MC. Validation of the polar team pro system for sprint speed with ice hockey players. J Strength Cond Res 36(12): 3468-3472, 2022-Commercially available player tracking sensors such as the Polar Team Pro system (PTPS) have been used to monitor athlete performance. Use during ice hockey practices and games has resulted in seemingly plausible and valid values for internal metrics (heart rate); however, external metrics (distance, speed, and acceleration) seem sporadic and counterintuitive. To determine the validity of the PTPS for measuring sprint speed with collegiate hockey players, 15 NCAA Division I male athletes (21.86 ± 1.04 years, 175.86 ± 6.78 cm, 80.58 ± 4.44 kg) participated in the study. Subjects wore a PTPS strap, at the level of the xiphoid process, and performed 2 sprints of 15.24 m (blue line to blue line) and 35.05 m (red line to the far blue line) in 3 conditions: indoor ice skating, indoor running, and outdoor running. Timing gates (TG) were used to determine sprint times, which allowed for manual calculation of speed. Speed values from the PTPS and TG were compared using paired-samples t -tests, and an alpha level of 0.05, 2-sided, was set a priori as a significance level. For indoor ice skating, PTPS significantly underestimated speeds at both distances ( p < 0.001). However, PTPS significantly overestimated speeds for indoor and outdoor sprints at both distances ( p ≤ 0.001). The PTPS is not accurate for measuring short-distance linear sprint speed or linear sprint speed during indoor ice skating. The inconsistency in speed values needs to be taken into consideration when using the system for science-based training because inaccurate speed values may negatively affect other external performance metrics provided by PTPS.


Assuntos
Desempenho Atlético , Hóquei , Corrida , Patinação , Masculino , Humanos , Hóquei/fisiologia , Patinação/fisiologia , Desempenho Atlético/fisiologia , Aceleração
4.
J Strength Cond Res ; 33(4): 1035-1042, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30908458

RESUMO

Whitehead, PN, Conners, RT, and Shimizu, TS. The effect of in-season demands on lower-body power and fatigue in male collegiate hockey players. J Strength Cond Res 33(4): 1035-1042, 2019-The purpose of this study was to determine the effect of in-season demands, such as games, travel, and the subsequent fatigue related to these demands, on lower-body power (LBP) in collegiate hockey players. Two jump protocols (countermovement and squat jumps) were used to calculate LBP. Twenty-seven NCAA (National Collegiate Athletic Association) Division I male hockey players (age, 21.9 ± 1.4 years; height, 176.7 ± 6.5 cm; body mass, 81.4 ± 7.9 kg) participated in the study that spanned 18 weekly sessions. At each session, participants completed an 8-item fatigue questionnaire, which provided a cumulative numerical value for fatigue (total score of fatigue [TSF]), before performing maximal jumps on a contact mat. General linear modeling was conducted to compare dependent variables (jump height, relative LBP, TSF) across weekly sessions. Pearson product-moment correlation coefficients were calculated to examine the relationship between jump height and LBP across the jump protocols, as well as to examine the relationship of TSF with all measured variables. Jump height and LBP declined throughout the season with greater effect of travel observed following the longest travel sequence (p ≤ 0.05; η ≥ 0.60). Differences in TSF were observed from baseline (p ≤ 0.037), and a large effect was seen for away trips that included air travel (d ≥ 1.35). Total score of fatigue had a negative correlation with jump height and power during both jumps (r ≤ -0.742; p < 0.001). In conclusion, the in-season demands of ice hockey resulted in significant reductions in LBP throughout a hockey season, and the 8-item fatigue questionnaire is a sensitive tool reflective of athletic performance measures.


Assuntos
Desempenho Atlético/fisiologia , Fadiga/fisiopatologia , Hóquei/fisiologia , Extremidade Inferior/fisiopatologia , Viagem , Teste de Esforço , Humanos , Estudos Longitudinais , Masculino , Força Muscular , Inquéritos e Questionários , Adulto Jovem
5.
J Strength Cond Res ; 32(4): 1166-1173, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28445228

RESUMO

Johnson, CD, Whitehead, PN, Pletcher, ER, Faherty, MS, Lovalekar, MT, Eagle, SR, and Keenan, KA. The relationship of core strength and activation and performance on three functional movement screens. J Strength Cond Res 32(4): 1166-1173, 2018-Current measures of core stability used by clinicians and researchers suffer from several shortcomings. Three functional movement screens appear, at face-value, to be dependent on the ability to activate and control core musculature. These 3 screens may present a viable alternative to current measures of core stability. Thirty-nine subjects completed a deep squat, trunk stability push-up, and rotary stability screen. Scores on the 3 screens were summed to calculate a composite score (COMP). During the screens, muscle activity was collected to determine the length of time that the bilateral erector spinae, rectus abdominis, external oblique, and gluteus medius muscles were active. Strength was assessed for core muscles (trunk flexion and extension, trunk rotation, and hip abduction and adduction) and accessory muscles (knee flexion and extension and pectoralis major). Two ordinal logistic regression equations were calculated with COMP as the outcome variable, and: (a) core strength and accessory strength, (b) only core strength. The first model was significant in predicting COMP (p = 0.004) (Pearson's Chi-Square = 149.132, p = 0.435; Nagelkerke's R-Squared = 0.369). The second model was significant in predicting COMP (p = 0.001) (Pearson's Chi-Square = 148.837, p = 0.488; Nagelkerke's R-Squared = 0.362). The core muscles were found to be active for most screens, with percentages of "time active" for each muscle ranging from 54-86%. In conclusion, performance on the 3 screens is predicted by core strength, even when accounting for "accessory" strength variables. Furthermore, it seems the screens elicit wide-ranging activation of core muscles. Although more investigation is needed, these screens, collectively, seem to be a good assessment of core strength.


Assuntos
Teste de Esforço/métodos , Força Muscular/fisiologia , Músculo Esquelético/fisiologia , Equilíbrio Postural/fisiologia , Adolescente , Adulto , Estudos Transversais , Eletromiografia/métodos , Exercício Físico/fisiologia , Feminino , Humanos , Masculino , Movimento/fisiologia , Contração Muscular/fisiologia , Amplitude de Movimento Articular , Tronco/fisiologia , Adulto Jovem
6.
J Strength Cond Res ; 28(3): 587-91, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23942165

RESUMO

This study analyzed the top 3 successful snatch attempts by individual lifters in each weight class at a U.S. National Championship weightlifting meet. Two-dimensional (2-D) body position and characteristics of the lifts were compared via 2D video analysis in groups of lifters who displaced forward, showed no displacement, or displaced backward to receive the bar. No significant group differences (p > 0.05) were noted for body mass, bar mass, or hip angle. The rearward displacement group had a significantly greater horizontal distance between the shoulder and heel at the end of the pull (determined as the point where the bar ceases to accelerate vertically). Hip angles for the no displacement group had a small-to-moderate effect size (0.50) in comparison to the forward displacement group, but they only showed a small effect size (0.17) when compared with the rearward displacement group. The forward displacement group showed a small-to-moderate effect size compared with both the no displacement group (0.51) and the rearward displacement group (0.55) concerning the horizontal distance from the shoulder to the heel. These data seem to suggest that rearward displacement in the drop-under phase in the snatch is not detrimental to performance and actually seems to be a preferred technique in U.S. national level lifters. In addition to evidence that rearward displacement is exhibited in elite lifters and is coached globally, it seems this is the preferred technique in international competitions. This technique may be considered a viable variation of the snatch by coaches and athletes of all levels.


Assuntos
Desempenho Atlético/fisiologia , Movimento/fisiologia , Levantamento de Peso/fisiologia , Adulto , Fenômenos Biomecânicos , Peso Corporal , Estudos Transversais , Articulação do Quadril/fisiologia , Humanos , Masculino , Postura , Estados Unidos , Gravação em Vídeo , Adulto Jovem
7.
Heliyon ; 9(5): e15832, 2023 May.
Artigo em Inglês | MEDLINE | ID: mdl-37215855

RESUMO

Increasing urbanization and industrialization are impacting on water quality globally. In the Awash River basin, Ethiopia, these drivers are impacting on water quality with further impacts created due to changes in water management releasing geogenic contaminants. The resulting water quality has potential to cause significant ecological and human health impacts. The physicochemical and heavy metals saptio-temporal variability and their associated risks to human health and ecology were assessed across twenty sampling stations in the Awash River basin. Over twenty-two physicochemical and ten heavy metals parameters were analyzed using different instruments including inductively coupled plasma mass spectrometer (ICP-MS). Elevated levels of heavy metals (As, V, Mo, Mn, and Fe) were detected in the surface water, surpassing the drinking water quality standards set by the World Health Organization (WHO). Seasonal variation was evident with peak concentration of As, Ni, Hg, and Cr were recorded in the dry season. A water quality index, hazard quotient, hazard index, heavy metal pollution index and heavy metal evaluation index were formulated to assess the potential risks to both human health and the environment. The highest values of heavy metal pollution index (HPI) above the threshold (>100) were observed in stations at Lake Beseka with HPI values ranged from 105 to 177. Similarly, the highest values of the heavy metals evaluation index (HEI) were observed in stations situated at cluster 3. The evaluation of health risk that is not related to cancer through hazard quotient demonstrated that in the case of both dermal and ingestion contact, cluster C3 > C1 > C4 > C2 and C3 > C4 > C2 > C1 were observed in children and adults, respectively. Overall, measures to reduce potential pollution risks must be taken in accordance with the standards in the river basin. Nevertheless, further research on the toxicity of heavy metals that pose risks to human health is also necessary.

8.
J Strength Cond Res ; 26(5): 1215-25, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-22228173

RESUMO

Drop vertical jumps (DVJs) stimulate enhanced countermovement loading as would occur with a run-up before jumping. A variety of performance variables have been associated with DVJ performance including ground contact time (GCT), reactive strength index (RSI), eccentric utilization ratio (EUR), and elasticity index (EI). This study examined the stability reliability and precision of these variables and their associations with DVJ displacement in trained men and women. The EUR and EI measures were redundant, so only EUR findings were reported. Except for EUR, data for all variables were both reliable and precise (intraclass correlation coefficient ≥ 0.70, coefficient of variation [CV%] ≤ 15.0) although EUR data were precise (CV% ≤ 15.0). Correlations with DVJ displacement were low for GCT, moderate for RSI, and negligible for EUR. Therefore, GCT and EUR likely represent unique performance characteristics not related to DVJ displacement. Furthermore, the variability in DVJ performance accounted for by RSI may primarily reflect the inclusion of displacement as the numerator in the quotient for calculating it.


Assuntos
Desempenho Atlético/fisiologia , Movimento , Força Muscular , Aceleração , Adolescente , Adulto , Feminino , Humanos , Masculino , Músculo Esquelético/fisiologia , Adulto Jovem
9.
Mil Med ; 177(11): 1417-25, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-23198525

RESUMO

The current U.S. Navy Physical Readiness Test (PRT) measures aerobic fitness and muscular endurance via a 1 1/2 mile run and curl-up/push-up tests, respectively. Nine new modalities were recommended to either replace or supplement the current PRT. Personnel and civilians (N = 179) were recruited from a local Navy base and participated in all modalities (duplicate if possible) over 4 weeks following familiarization sessions. Subjects performed single-leg plank, single-leg wall squat, cadence push-ups, leg/hip dynamometer, standing long jump, and pro-agility test. Cardiovascular modalities were also performed via the 300-yard shuttle, 2-km row, and 5-km bike. Performance in the modalities was correlated to the subjects' existing PRT scores as well as within the new modalities. Although most modalities could not be concretely recommended, the plank and wall squat were eliminated from consideration because of poor reliability scores. The strongest correlation existed between the standing long jump and pro-agility test for the entire sample. Correlation scores were also analyzed by gender. The cardiovascular modalities did not have strong enough scores to elicit a recommendation to replace the 1 1/2 mile run, but future considerations for similar testing would be to collect scores for the existing modalities in addition to the proposed modalities.


Assuntos
Teste de Esforço/métodos , Militares , Medicina Naval/métodos , Resistência Física , Aptidão Física/fisiologia , Adulto , Feminino , Humanos , Masculino , Estados Unidos
10.
Sci Total Environ ; 816: 151638, 2022 Apr 10.
Artigo em Inglês | MEDLINE | ID: mdl-34774956

RESUMO

In contrast to marine ecosystems, the toxicity impact of microplastics in freshwater environments is poorly understood. This contribution reviews the literature on the range of effects of microplastics across and between trophic levels within the freshwater environment, including biofilms, macrophytes, phytoplankton, invertebrates, fish and amphibians. While there is supporting evidence for toxicity in some species e.g. growth reduction for photoautotrophs, increased mortality for some invertebrates, genetic changes in amphibians, and cell internalization of microplastics and nanoplastics in fish; other studies show that it is uncertain whether microplastics can have detrimental long-term impacts on ecosystems. Some taxa have yet to be studied e.g. benthic diatoms, while only 12% of publications on microplastics in freshwater, demonstrate trophic transfer in foodwebs. The fact that just 2% of publications focus on microplastics colonized by biofilms is hugely concerning given the cascading detrimental effects this could have on freshwater ecosystem function. Multiple additional stressors including environmental change (temperature rises and invasive species) and contaminants of anthropogenic origin (antibiotics, metals, pesticides and endocrine disruptors) will likely exacerbate negative interactions between microplastics and freshwater organisms, with potentially significant damaging consequences to freshwater ecosystems and foodwebs.


Assuntos
Microplásticos , Poluentes Químicos da Água , Animais , Biota , Ecossistema , Monitoramento Ambiental , Água Doce , Plásticos/toxicidade , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/toxicidade
11.
Artigo em Inglês | MEDLINE | ID: mdl-36078215

RESUMO

The use of facial coverings has been amplified during the COVID-19 pandemic as a means to minimize the spread of disease. However, facial coverings may impede ventilation during high-intensity activity, leading to a reduction in cardiopulmonary exercise capacity. Thus, the purpose of this study was to determine the acute impact of different facial coverings on exercise performance in college-aged individuals during a 300-yard shuttle. It was hypothesized that the lowest heart rate (HR), completion time (CT), and rate of perceived exertion (RPE) would occur with no mask. Furthermore, it was hypothesized the SHEMA97 mask would have lower HR, CT, and RPE compared to surgical and fabric masks. Results showed the use of the fabric mask resulted in significantly higher HR compared to no mask (p = 0.006). The SHEMA97 mask resulted in faster CT and lower RPE compared to both the fabric and surgical masks (p < 0.001). All mask conditions yielded significantly higher levels of perceived discomfort than wearing no mask (p < 0.05). While the use of facial coverings can help prevent the spread of disease, their use during exercise may pose limitations to performance; however, the ability of the SHEMA97 to provide minimal changes to CT and RPE provides a promising option.


Assuntos
COVID-19 , Pandemias , Adulto , Anaerobiose , Exercício Físico , Humanos , Máscaras , Pandemias/prevenção & controle , Adulto Jovem
12.
Int J Exerc Sci ; 14(4): 230-238, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34055165

RESUMO

One method to measure body composition that is gaining popularity is the BodyMetrix™, which uses A-mode ultrasound. A-mode ultrasound, when used with the BodyMetrix™, has been found to be a reliable and a cost-effective tool for measuring overall body fat percentage. Furthermore, the portability and short duration testing features of the BodyMetrix™, allows for testing of a large group of athletes inside or outside of a clinical setting. Despite these advantages, research regarding the BodyMetrix™ is limited and has primarily focused on the seven-site testing technique. However, the three-site technique allows for faster testing and a reduction of time needed to test an entire team or multiple sports teams. Thus, the purpose of this study was to compare the three-site and seven-site methods using the BodyMetrix™ to determine body fat percentage in female collegiate athletes. It was hypothesized that body fat percentage determined via the seven-site method would be different from those obtained by three-site measurement technique. Study participants included 40 National Collegiate Athletic Association (NCAA) Division-II female athletes from volleyball, soccer, and softball teams. The Jackson Pollock three-site (thigh, suprailiac, triceps) and seven-site (thigh, suprailiac, triceps, abdominal, subscapular, chest, and axilla) equations were used to determine body fat percentage values. The time required to perform the three-site and seven-site measurements were also recorded. A paired samples t-test was used to assess if there was a difference between the three-site and the seven-site body fat percentage measurements with the use of the BodyMetrix™ . The three-site method (23.21 ± 3.61) was significantly lower (p < 0.001) compared to the seven- site method (25.75 ± 4.39). On average, the three-site technique took 2 minutes and 13 seconds less than the seven-site technique.

13.
J Biomech ; 124: 110553, 2021 07 19.
Artigo em Inglês | MEDLINE | ID: mdl-34161842

RESUMO

Aquatic therapy for rehabilitation can be performed in a variety of environments, which can vary from a traditional swimming pool to a self-contained underwater treadmill. While kinematic analysis has been performed in large volume swimming pools using specific underwater motion capture systems, researchers may only have access to a land-based motion-capture system, which is not waterproof. Additionally, underwater motion capture systems may not fit within the confines of a smaller underwater treadmill. Thus, the purpose of this study was to design and analyze methodology to quantify lower limb kinematics during an aquatic treadmill session, using a land-based motion capture system. Kinematics of lower limb motion at different speeds was studied while walking on an underwater treadmill in comparison to walking on the same treadmill without water (empty tank). The effects of the presence of water on walking kinematics was analyzed and interpreted using parametric and non-parametric testing procedures. The results suggest significant influences of speed on knee and ankle angles (p < 0.05) in both dryland and aquatic scenarios. Knee and ankle angle measures revealed no significant differences between the dryland and water treadmill scenarios (p > 0.05). The increased time requirement in water for the full gait cycle found in this study indicates influence of resistive effects. This finding can be especially suited for muscle strengthening and stabilizing treatments for lower limbs. Also, a framework was developed to realize a potential methodology to use land-based motion capture cameras to successfully analyze the kinematics of gait in constrained aquatic volumes.


Assuntos
Marcha , Caminhada , Fenômenos Biomecânicos , Teste de Esforço , Movimento (Física)
14.
Children (Basel) ; 8(8)2021 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-34438581

RESUMO

Preventative measures taken worldwide to decrease the transmission of COVID-19 have had a tremendous impact on youth. Following social restrictions, youth with and without physical disabilities are engaging in less physical activity, more increased sedentary behavior, and poor sleep habits. Specifically, youth wheelchair users (YWU) are likely disproportionately affected by COVID- 19 and have a higher risk of contraction due to underlying comorbidities. While we cannot control all of the negative long-term implications of COVID-19 for YWU, participation in positive 24-h activity behaviors can decrease chronic disease risk and the likelihood of long-term complications resulting from infection. This commentary is to extend the discourse on the importance of 24-h activity behaviors by focusing on YWU. Specifically, we discuss the importance of chronic disease prevention, provide a brief overview of 24-h activity behaviors, and outline some of the lessons that can be learned from the COVID-19 pandemic.

15.
Sci Total Environ ; 755(Pt 1): 142468, 2021 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-33032131

RESUMO

The livelihoods of millions of people living in the world's deltas are deeply interconnected with the sediment dynamics of these deltas. In particular a sustainable supply of fluvial sediments from upstream is critical for ensuring the fertility of delta soils and for promoting sediment deposition that can offset rising sea levels. Yet, in many large river catchments this supply of sediment is being threatened by the planned construction of large dams. In this study, we apply the INCA hydrological and sediment model to the Mekong River catchment in South East Asia. The aim is to assess the impact of several large dams (both existing and planned) on the suspended sediment fluxes of the river. We force the INCA model with a climate model to assess the interplay of changing climate and sediment trapping caused by dam construction. The results show that historical sediment flux declines are mostly caused by dams built in PR China and that sediment trapping will increase in the future due to the construction of new dams in PDR Lao and Cambodia. If all dams that are currently planned for the next two decades are built, they will induce a decline of suspended sediment flux of 50% (47-53% 90% confidence interval (90%CI)) compared to current levels (99 Mt/year at the delta apex), with potentially damaging consequences for local livelihoods and ecosystems.

16.
Sci Rep ; 10(1): 14408, 2020 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-32873881

RESUMO

Quantitatively determining in vivo achievable drug concentrations in targeted organs of animal models and subsequent target engagement confirmation is a challenge to drug discovery and translation due to lack of bioassay technologies that can discriminate drug binding with different mechanisms. We have developed a multiplexed and high-throughput method to quantify drug distribution in tissues by integrating high content screening (HCS) with U-Net based deep learning (DL) image analysis models. This technology combination allowed direct visualization and quantification of biologics drug binding in targeted tissues with cellular resolution, thus enabling biologists to objectively determine drug binding kinetics.


Assuntos
Caderinas/imunologia , Carbocianinas , Aprendizado Profundo , Corantes Fluorescentes , Ensaios de Triagem em Larga Escala/métodos , Processamento de Imagem Assistida por Computador/métodos , Imunoconjugados/metabolismo , Animais , Caderinas/metabolismo , Colo/metabolismo , Descoberta de Drogas/métodos , Intestino Delgado/metabolismo , Camundongos , Distribuição Tecidual
17.
Zootaxa ; 4555(4): 588-594, 2019 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-30790940

RESUMO

The Oriental burrower bug Macroscytus subaeneus (Dallas, 1851) (Hemiptera: Heteroptera: Cydnidae: Cydninae), presently known only from single localities in Flores (Indonesia), the Philippines and Thailand, is recorded from the United Kingdom (hereafter U.K.). A single female specimen was collected on the semi-rural edge of Bath city by a Starling (Sturnus vulgaris L.) and taken to its nest from which it was retrieved during 2015. The specimen is illustrated and its diagnostic characters are provided. We hypothesize that this specimen could have been imported with a shipping container from Thailand.


Assuntos
Heterópteros , Animais , Europa (Continente) , Feminino , Indonésia , Filipinas , Tailândia , Reino Unido
18.
J Biomech ; 89: 139-142, 2019 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-31030892

RESUMO

While the capabilities of land-based motion capture systems in biomechanical applications have been previously reported, the possibility of using motion tracking systems externally to reconstruct markers submerged inside an aquatic environment has been under explored. This study assesses the ability of a motion capture system (Vicon T40s) arranged externally to track a retro-reflective marker inside a glass tank filled with water and without water. The reflective tape used for marker creation in this study was of Safety of Life at Sea (SOLAS) grade as the conventional marker loses its reflective properties when submerged. The overall trueness calculated based on the mean marker distance errors, varied between 0.257 mm and 0.290 mm in different mediums (air, glass and water). The overall precision calculated based on mean standard deviation of mean marker distances at different locations varied between 0.046 mm and 0.360 mm in different mediums. Our results suggest, that there is no significant influence of the presence of water on the overall static accuracy of the marker center distances when markers were made of SOLAS grade reflective tape. Using optical motion tracking systems for evaluating locomotion in aquatic environment can help to better understand the effects of aquatic therapy in clinical rehabilitation, especially in scenarios that involve equipment, such as an underwater treadmill which generally have constrained capture volumes for motion capture.


Assuntos
Movimento (Física) , Dispositivos Ópticos , Água , Algoritmos , Fenômenos Mecânicos
19.
Sci Total Environ ; 646: 1459-1467, 2019 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-30235631

RESUMO

Pharmaceuticals, personal care products (PPCPs), and artificial sweeteners (ASWs) are contaminants of emerging concern commonly found in the aquatic environments. In India, studies reporting environmental occurrence of these contaminants are scarce. In this study, we investigated the occurrence and distribution of 15 PPCPs and five ASWs in the river and groundwater (used untreated as drinking water) at several sites along the Ganges River. Based on the measured groundwater concentrations, we estimated the life-long human health risk from exposure to PPCPs through drinking. In addition, we estimated the risk of exposure to PPCPs and ASWs in the river water for aquatic organisms. The sum of detected PPCPs in the river water ranged between 54.7-826 ng/L, with higher concentrations in the severely anthropogenically influenced middle and lower reaches of the Ganges. The highest concentration among the PPCPs in the river water was of caffeine (743 ng/L). The sum of detected ASWs in river water ranged between 0.2-102 ng/L. Similar to PPCPs, the sum of ASWs in the river water was higher in the middle and lower reaches of the Ganges. In groundwater, the sum of detected PPCPs ranged between 34-293 ng/L, whereas of ASWs ranged between 0.5-25 ng/L. Negligible risk for humans was estimated from PPCPs in the drinking groundwater sources along the Ganges River, whereas moderate risks to PPCPs and ASWs (namely: caffeine, sulfamethoxazole, triclocarban, triclosan, and sucralose) were estimated for aquatic organisms in the Ganges River.


Assuntos
Cosméticos/análise , Monitoramento Ambiental , Preparações Farmacêuticas/análise , Edulcorantes/análise , Poluentes Químicos da Água/análise , Água Potável/química , Água Subterrânea/química , Humanos , Índia , Medição de Risco , Rios/química
20.
Sci Total Environ ; 400(1-3): 369-78, 2008 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-18644618

RESUMO

We review the current status of knowledge regarding the role that flow parameters play in controlling the macrophyte communities of temperate lowland rivers. We consider both direct and indirect effects and the interaction with other factors known to control macrophyte communities. Knowledge gaps are identified and implications for the management of river systems considered. The main factors and processes controlling the status of macrophytes in lowland rivers are velocity (hence also discharge), light, substrate, competition, nutrient status and river management practices. We suggest that whilst the characteristics of any particular macrophyte community reflect the integral effects of a combination of the factors, fundamental importance can be attributed to the role of discharge and velocity in controlling instream macrophyte colonisation, establishment and persistence. Velocity and discharge also appear to control the relative influence of some of the other controlling factors. Despite the apparent importance of velocity in determining the status of macrophyte communities in lowland rivers, relatively little is understood about the nature of the processes controlling this relationship. Quantitative knowledge is particularly lacking. Consequently, the ability to predict macrophyte abundance and distribution in rivers is still limited. This is further complicated by the likely existence of feedback effects between the growth of macrophytes and velocity. Demand for water resources increases the pressure on lowland aquatic ecosystems. Despite growing recognition of the need to allocate water for the needs of instream biota, the inability to assess the flow requirements of macrophyte communities limits the scope to achieve this. This increases the likelihood of overexploitation of the water resource as other users, whose demands are quantifiable, are prioritised.


Assuntos
Desenvolvimento Vegetal , Rios , Movimentos da Água , Biomassa , Dinâmica Populacional
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