RESUMO
We use a single-photon detector to detect the spin Hall effect of light (SHEL) of a quasi-single-photon beam obtained in this Letter. The physics of the spin Hall effect and its quantum weak measurement method with a dimensionless pointer are elucidated through particle number representation. Our weak measurement scheme obviates the necessity of high-resolution single-photon array detectors. Consequently, we have successfully observed the spin Hall effect within a 20 ns temporal window using a position-resolution-independent single-photon detector with remarkably low-noise levels. The weak measurement of the dimensionless pointer presented in this Letter boosts both the detection accuracy and the response speed of the photonics spin Hall effect, thereby contributing significantly to fundamental theoretical research in spin photonics and precise measurements of physical property parameters.
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The health risks of Decabromodiphenyl ethane (DBDPE) with its cardiovascular toxicity, liver toxicity and cytotoxicity had been generally acknowledged. However, the influence on gut microbiome and short-chain fatty acids (SCFAs) metabolism caused by DBDPE exposure remained unknown. In this study, three exposure groups (5, 50, 500 mg/L) and control group were used to investigate the effect of DBDPE by using simulator of the human intestinal microbial ecosystem (SHIME). 16S rRNA gene high-throughput sequencing illustrated that high dose DBDPE exposure increased the α-diversity of gut microbiota, while reduced the abundance of Firmicutes and Proteobacteria. In addition, the low dose (5 mg/L) DBDPE inhibited the increasing of SCFAs, but the medium and high dose (50 and 500 mg/L) DBDPE promoted the advancement, especially in ascending colon. Notably, DBDPE exposure lead a similar changing of acetic acid and butyric acid contents in different sections of the colon. This study confirmed the alternation of composition and metabolic function in gut microbial community due to DBDPE exposure, indicating an intestinal damage and appealing for more attention concentrated on the health effects of DBDPE exposure.
Assuntos
Retardadores de Chama , Microbioma Gastrointestinal , Bromobenzenos , Ecossistema , Retardadores de Chama/toxicidade , Humanos , RNA Ribossômico 16S/genéticaRESUMO
A Gram-positive, non-motile, non-spore-forming and short rod-shaped actinomycete strain, designated GA224T, was isolated from electronic waste-associated bioaerosols. The optimal growth conditions for this isolate, a facultatively anaerobic bacterium, were 37 °C and pH 8.0. The cell-wall peptidoglycan type was B2γ, with 2,4-diaminobutyric acid (DAB) as the diamino acids, while the major menaquinone was MK-12. The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids, unidentified glycolipids and an unidentified lipid. The major cellular fatty acids were anteiso-C15:0 and iso-C16:0. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain GA224T fell within the genus Microcella. The draft genome of strain GA224T comprised 2,495,189 bp with a G + C content of 72.2 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between strain GA224T and the type strain of the type species of Microcella species were lower than 95% and 70%, respectively. Based on the phenotypic, chemotaxonomic and genomic data, strain GA224T represents a novel species, for which the name Microcella aerolata sp. nov. is proposed, with GA224T as the type strain (= GDMCC 1.2165 T = JCM 34462 T).
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Actinomycetales , Resíduo Eletrônico , Actinomycetales/genética , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/análise , Fosfolipídeos/análise , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/químicaRESUMO
Many organic pollutants attract public health concern due to their genotoxicity. To investigate the genotoxicity of organic matter in surface water of the Pearl River Delta (PRD). Organic substances of 24 samples (dry and wet season) from North River, West River and East River were extracted from 60 L source water by XAD-2 macroporous resin. DNA damage effect of organic extracts was tested in human derived liver cells (HL-7702), using single cell gel electrophoresis (SCGE) assay. The results showed that 100% organic extracts (24/24) could induce DNA damage in HL-7702 cells when the concentration was above 1.0 L surface water/ml culture, no significant difference of DNA damage between dry and wet seasons was observed. The organic substance-induced DNA damage in HL-7702 cells was significantly (P < 0.05) correlated with the contents of Dissolved Organic Carbon in both seasons and Total Suspended Solids in dry season. In conclusion, organic extracts induced genetic damage in HL-7702 cells, indicating potential genotoxicity of organic pollutants of surface water from PRD, South China.
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Dano ao DNA , Rios , Poluentes Químicos da Água , Linhagem Celular , China , Monitoramento Ambiental , Humanos , Fígado/química , Estações do Ano , Água , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/toxicidadeRESUMO
A Gram-positive, endospore-forming, rod-shaped bacterium with a single flagellum, and a motile strain, designated CX253, was isolated from bioaerosols. The isolate is facultatively anaerobic, is able to grow at 25-45 â (optimum 37 â) and pH 6.5-10.0 (optimum 7.5), and can tolerate up to 5.0% NaCl (w/v) under aerobic conditions. The diagnostic diamino acid in the cell wall of strain CX253T is meso-diaminopimelic acid, while major isoprenoid quinone is menaquinone 6 (MK-6) along with a smaller amount of MK-7 (20%). The polar lipid profile is composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phospholipids and glycolipids. The major cellular fatty acid is iso-C15:0 and anteiso-C15:0. Phylogenetic analysis based on 16S rRNA gene and genome sequence grouped strain CX253T into the genus Bacillus. The strain was most closely related to Bacillus thermotolerans CCTCC AB 2012108 T by comparison of 16S rRNA gene sequence (97.2% similarity) and to Bacillus wudalianchiensis CCTCC AB 2015266 T by comparison of gyrB gene sequence (80.1% similarity). The draft genome of strain CX253T comprised 3,929,195 bp with a G + C content of 43.3 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between strain CX253T and phylogenetically related Bacillus species were lower than 95% and 70%, respectively. Thus, the polyphasic evidence generated through phenotypic, chemotaxonomic and genomic methods confirmed that strain CX253T (= GDMCC 1.1608 T = KACC 21318 T) was a novel species of the genus Bacillus, for which the name Bacillus aerolatus sp. nov. is proposed.
Assuntos
Aerossóis , Bacillus/classificação , Microbiologia Ambiental , Bacillaceae/genética , Bacillus/genética , Bacillus/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , Parede Celular/química , Ácido Diaminopimélico/análise , Ácidos Graxos/análise , Glicolipídeos/análise , Hibridização de Ácido Nucleico , Fosfolipídeos/análise , Filogenia , RNA Ribossômico 16S/genética , Especificidade da EspécieRESUMO
The mechanism of arsenic-induced skin carcinogenesis is not yet fully understood. Chromosomal instability contributes to aneuploidy and is a driving force in carcinogenesis. Arsenic causes mitotic arrest and induces aneuploidy. hsa-miR-186 overexpression is associated with metastatic cancers as well as arsenic-induced squamous cell carcinoma and is reported to target several mitotic regulators. Decreased levels of these proteins can dysregulate chromatid segregation contributing to aneuploidy. This work investigates the potential aneuploidogenic role of hsa-miR-186 in arsenic carcinogenesis. Clones of immortalized human keratinocytes (HaCaT) stably transfected with a hsa-miR-186 expression or empty vector were isolated. Three clones with high and low hsa-miR-186 expression determined by RT-qPCR were selected for further analysis and cultured with 0 or 100â¯nM NaAsO2 for 8â¯weeks. Analysis of mitoses revealed that chromosome number and structural abnormalities increased in cells overexpressing hsa-miR-186 and were further increased by arsenite exposure. Double minutes were the dominant structural aberrations. The peak number of chromosomes also increased. Cells with >220 to >270 chromosomes appeared after 2â¯months in hsa-miR-186 overexpressing cells, indicating multiple rounds of endomitosis had occurred. The fraction of cells with increased chromosome number or structural abnormalities did not increase in passage matched control cells. Levels of selected target proteins were determined by western blot. Expression of BUB1, a predicted hsa-miR-186 target was suppressed in hsa-miR-186 overexpressing clones, but increased with arsenite exposure. CDC27 remained constant under all conditions. These results suggest that overexpression of miR-186 in arsenic exposed tissues likely induces aneuploidy contributing to arsenic-induced carcinogenesis.
Assuntos
Arsênio/efeitos adversos , Arsenitos/efeitos adversos , Instabilidade Cromossômica/genética , Queratinócitos/efeitos dos fármacos , MicroRNAs/genética , Carcinogênese/efeitos dos fármacos , Carcinogênese/genética , Linhagem Celular , HumanosRESUMO
Voltage-gated sodium channel activity enhances the motility and oncogene expression of metastasic cancer cells that express a neonatal alternatively spliced form of the NaV1.5 isoform. We reported previously that FS50, a salivary protein from Xenopsylla cheopis, showed inhibitory activity against the NaV1.5 channel when assayed in HEK 293T cells and antiarrhythmia effects on rats and monkeys after induction of arrhythmia by BaCl2. This study aims to identify the effect of FS50 on voltage-gated sodium channel activity and the motility of MDA-MB-231 human breast cancer cells in vitro. NaV1.5 was abnormally expressed in the highly metastatic breast cancer cell line MDA-MB-231, but not in the MCF-7 cell line. FS50 significantly inhibited sodium current, migration, and invasion in a dose-dependent manner, but had no effect on the proliferation of MDA-MB-231 cells at the working concentrations (1.5-12 µmol/l) after a long-term treatment for 48 h. Meanwhile, FS50 decreased NaV1.5 mRNA expression without altering the total protein level in MDA-MB-231 cells. Correspondingly, the results also showed that MMP-9 activity and the ratio of MMP-9 mRNA to TIMP-1 mRNA were markedly decreased by FS50. Taken together, our findings highlighted for the first time an inhibitory effect of a salivary protein from a blood-feeding arthropod on breast cancer cells through the NaV1.5 channel. Furthermore, this study provided a new candidate leading molecule against antitumor cells expressing NaV1.5.
Assuntos
Neoplasias da Mama/tratamento farmacológico , Canal de Sódio Disparado por Voltagem NAV1.5/efeitos dos fármacos , Proteínas e Peptídeos Salivares/farmacologia , Xenopsylla/metabolismo , Animais , Neoplasias da Mama/patologia , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Relação Dose-Resposta a Droga , Feminino , Humanos , Células MCF-7 , Metaloproteinase 9 da Matriz/genética , Canal de Sódio Disparado por Voltagem NAV1.5/genética , RNA Mensageiro/metabolismo , Proteínas e Peptídeos Salivares/administração & dosagem , Proteínas e Peptídeos Salivares/genética , Fatores de Tempo , Inibidor Tecidual de Metaloproteinase-1/genéticaRESUMO
It appears to be more practical and effective to prevent carcinogenesis by targeting the tumor promotion stage. Gap junctional intercellular communication (GJIC) is strongly involved in carcinogenesis, especially the tumor promotion stage. Considerable interest has been focused on the chemoprevention activities of soyasaponin (SS), which are major phytochemicals found in soybeans and soy products. However, less is known about the preventive effects of SS (especially SS with different chemical structures) against tumor promoter-induced inhibition of GJIC. We investigated the protective effects of SS-A1, SS-A2, and SS-I against hydrogen peroxide (H2O2)-induced GJIC inhibition and reactive oxygen species (ROS) production in Buffalo rat liver (BRL) cells. The present results clearly show for the first time that SS-A1, SS-A2, and SS-I prevent the H2O2-induced GJIC inhibition by scavenging ROS in BRL cells in a dose-dependent manner at the concentration range of from 25 to 100 µg/mL. Soyasaponins attenuated the H2O2-induced ROS through potentiating the activities of superoxide dismutase and glutathione peroxidase. This may be an important mechanism by which SS protects against tumor promotion. In addition, various chemical structures of SS appear to exhibit different protective abilities against GJIC inhibition. This may partly attribute to their differences in ROS-scavenging activities.
Assuntos
Comunicação Celular/efeitos dos fármacos , Sequestradores de Radicais Livres/farmacologia , Junções Comunicantes/efeitos dos fármacos , Hepatócitos/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismo , Saponinas/farmacologia , Animais , Catalase/metabolismo , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Ensaio Cometa , Dano ao DNA/efeitos dos fármacos , Junções Comunicantes/metabolismo , Glutationa Peroxidase/metabolismo , Hepatócitos/metabolismo , Peróxido de Hidrogênio/efeitos adversos , Peroxidação de Lipídeos/efeitos dos fármacos , Fígado/citologia , Fígado/efeitos dos fármacos , Malondialdeído/metabolismo , Ratos , Glycine max/química , Superóxido Dismutase/metabolismoRESUMO
Sensitive magnetometer technology is desirable for biomagnetic field detection and geomagnetic field measuring. Signal amplification materials such as magneto-optical crystals or ceramics are crucial for enhancing detection sensitivity, but severe optical scattering and low Verdet constant further limit its application. To develop high-sensitivity magnetometers for quantum weak measurement schemes, we have conducted investigations on the powder calcining dynamics and prepared a series of high-optical-quality (Ho/Dy)2Zr2O7 transparent ceramic samples. The Verdet constant of magneto-optical materials was measured across a continuous wavelength spectrum, exhibiting a peak at 283 ± 5 rad/(T·m). We further established an electron transition mechanism to elucidate the exceptional magneto-optical attributes of dysprosium. In addition, samples demonstrated superior performance in weak-value amplification, reaching a low detectable magnetic field threshold of 3.5 × 10-8 T and continuously worked over 6 h with high stability. Our work developed a highly sensitive magnetometer using optimized magneto-optical ceramics and provided guidance on design, fabrication, and application for magneto-optical ceramics in quantum weak measurement.
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Streptococcus pneumoniae (Spn) is the most common respiratory pathogen causing community-acquired pneumonia. Probiotics represent a new intervention target for Spn infection. Hence, the discovery and development of new potential probiotic strains are urgently needed. This study was designed to investigate the beneficial effect and mechanism of a new bacterium named Roseicella aerolata GB24T that antagonizes Spn at cellular and animal levels. The results revealed that GB24T strain inhibited the growth of Spn on sheep blood agar plates, forming inhibition circles with a diameter of 20 mm. In cultured bronchial epithelium transformed with Ad 12-SV40 2B (BEAS-2B) cells, Spn infection induced an elevation in the expression levels of interleukin-1ß, interleukin-6, and tumor necrosis factor-α to 4.289 ± 0.709, 5.587 ± 2.670, and 5.212 ± 0.772 folds compared to healthy controls, respectively. Moreover, pre-infection with GB24T for 1.5 h almost eliminated the cellular inflammation caused by Spn infection. Additionally, male Sprague-Dawley rats infected with Spn were randomly allocated into two groups: GB24T pre-infection and Spn infection groups, with healthy rats as control. GB24T significantly alleviated inflammatory lung injury caused by Spn infection, which was associated with obvious changes in the abundance of gut microbiota and a trend toward enhanced secretion of short-chain fatty acids, especially acetic acid. Acetic acid was validated to be effective in alleviating inflammation due to Spn infection in cellular assays. Together, these findings highlight that GB24T strain is an important protective feature in the respiratory tract.
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In order to solve the problem of soil pollution caused by excess heavy metals, cadmium (Cd), a novel soil-washing agent organic chelating acid-based deep eutectic solvent (OCA-DES), was investigated for the removal of Cd from the contaminated soil of coking plant. Four kinds of OCA-DES were prepared by mixing choline chloride (Ch-Cl) with four organic chelating acids (citric acid, oxalic acid, tartaric acid, and malic acid), respectively, to compare their washing efficiency of Cd from soil. The effects of washing operation conditions on the Cd removal efficiency were investigated. Side effects of citric acid-based deep eutectic solvent (CA-DES) on soil were analyzed using scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared (FTIR). The results showed that CA-DES had the best Cd removal efficiency and could reach as high as 93.75%, under ideal conditions. CA-DES mainly washed extractable and reducible Cd. The CA-DES washing process has less side effects on soil and hardly eroded the soil or changed the mineral structure of the soil. The main roles of CA-DES washing Cd include (1) hydrogen bonds capturing Cd; (2) the carboxyl group(-COOH) forming soluble chelate with Cd; and (3) releasing H+ ions in exchange for Cd. This study demonstrates that CA-DES, a novel soil-washing agent, has excellent removal of cadmium from soil and is environment-friendly.
Assuntos
Cádmio , Poluentes do Solo , Cádmio/análise , Solo/química , Solventes Eutéticos Profundos , Detergentes , Ácido Cítrico/química , Solventes , Poluentes do Solo/análise , Quelantes/química , Compostos Orgânicos , ÁcidosRESUMO
Soil washing is a rapid and efficient method for heavy metals removal. In this study, a kind of novel environmentally friendly eluent is proposed, consisting of ethylenediamine tetra methylene phosphonic acid and saponin (E-S). In a response surface optimization design (RSOD), the operating parameters were optimized and ecological effects were investigated. Additionally, soil microbial diversity and composition were discussed. Finally, an optimal method for chelator recovery was presented. The single-factor and RSOD results showed that E-S had higher remediation efficiency for Pb-Cd contaminated soil with the best parameters of duration 240 min, temperature 40 °C, E/S ratio 1:1. Under these operating conditions, the removal rates of Pb and Cd were (72.2 ± 0.5)% and (61.2 ± 0.4)% for the artificially contaminated soil and (69.2 ± 0.1)% and (65.2 ± 0.2)% for in situ contaminated soil, respectively. The contents of Pb and Cd decreased from 562.36 mg/kg to 180.41 mg/kg and 80.96 mg/kg to 27.2 mg/kg respectively, and the soil ecological risk indexes reduced from 32% to 24% for Pb and 36%-27% for Cd. Owing to the efficient enrichment of Stenotrophomonas in soil, E-S has a potential of simultaneous removal effect on organic pollutants as well. Furthermore, the results revealed that E-S can be effectively recovered (>95%) by sodium sulfide precipitation.
Assuntos
Recuperação e Remediação Ambiental , Metais Pesados , Saponinas , Poluentes do Solo , Cádmio , Chumbo , Ácidos Fosforosos , Solo , Poluentes do Solo/análise , Metais Pesados/análiseRESUMO
Airborne microorganisms in the waste associated environments are more active and complex compared to other places. However, the diversity and structure of airborne bacteria in waste-associated environments are still not clearly understood. The purpose of this study was to assess airborne bacterial community in electronic waste dismantling site and a waste transfer station based on culture-dependent and culture-independent methods. A total of 229 isolates were obtained from four airborne sites collected from residential area, electronic industrial park, and office area in or near an electronic waste dismantling site and a waste transfer station in Southern China in the morning, afternoon, and evening. Most of the isolates were isolated from air for the first time and 14 potentially novel species were identified by Sanger sequencing. Bacterial communities in waste-associated bioaerosols were predominated by Proteobacteria and Bacteroidetes. Abundant genera (>1%) included Paracaedibacteraceae (uncultured EF667926), Ralstonia, Chroococcidiopsis, Chitinophagaceae (uncultured FN428761), Sphingobium, and Heliimonas. One-third of the species in these genera were uncultured approximately. Differences community structure existed in airborne bacterial diversity among different sampling sites. These results showed that waste-associated environments have unique bacterial diversity. Further studies on such environments could provide new insights into bacterial community.
Assuntos
Resíduo Eletrônico , Microbiota , Aerossóis/análise , Bactérias/genética , ChinaRESUMO
The gut microbiota has been shown to be highly involved in many vital physiological processes that play key roles in human health. The intergenerational transfer of Dechlorane Plus (DP) and the complex interaction between DP and microbiota has been poorly studied. Additionally, the structural and functional effects of DP on the gut microbiota have not been studied. This study aimed to investigate the DP transfer in Sprague-Dawley rats during pregnancy and the effects of DP exposure on gut microbiota, as detected by 16S rRNA gene sequencing. The results showed that excretion in feces is a very important elimination pathway of orally dosed DP. The main intergenerational transfer pathway of DP might be via lactation rather than transplacental transport. The 16S rRNA sequencing revealed that DP exposure could decrease the richness and diversity of gut microbiota, especially at the genus level. Furthermore, in DP exposure groups, the gut microbiota production of metabolites of short-chain fatty acids was dramatically increased. The results demonstrated that DP exposure not only altered the gut microbiota structures, but also immensely influenced metabolic functions, causing long-term impact to offspring. This data indicates that more attention should be paid to the long-term health effects related to DP exposure.
Assuntos
Microbioma Gastrointestinal , Animais , Ácidos Graxos Voláteis , Fezes , Feminino , Microbioma Gastrointestinal/genética , Humanos , Hidrocarbonetos Clorados , Compostos Policíclicos , Gravidez , RNA Ribossômico 16S/genética , Ratos , Ratos Sprague-DawleyRESUMO
BACKGROUND: Integrins are crucial anti-cancer therapy targets. We previously showed that tablysin-15 is an integrin antagonist with its Arg-Gly-Asp motif in a novel structural context. OBJECTIVE: Here we investigated the anti-cancer effects and mechanisms of action of tablysin-15 in melanoma cells. METHODS: Cell adhesion, competitive binding, cell viability, and ATP chemiluminescence assays were used to analyze the binding of tablysin-15 to αvß3 integrin and its phenotypic effects. Wound healing, transwells, and zymography were performed to detect motility and matrix metalloproteinase- 2/-9 activities. PARP and caspase-3 cleavage were used as apoptosis assays, while LDH release and flow cytometry were used for necrosis and cell cycle analysis. The expression of mRNAs and proteins of target molecules was measured by qRT-PCR and western blotting, respectively. RESULTS: Tablysin-15 dose-dependently inhibited the proliferation, migration, and invasion of M21 cells through integrin αvß3. The proliferation inhibition caused by tablysin-15 was attributable to G0/G1 phase arrest rather than apoptosis or necrosis. Furthermore, tablysin-15 suppressed MMP-2/- 9 activities and the mRNA expression of MMP-2/-9 and COX-2 but was upregulated TIMP-1 in M21 cells. Meanwhile, tablysin-15 suppressed the expression of cyclin D1/E and CDK 2/6, the phosphorylation of FAK, Akt, and ERK, and nuclear translocation of NF-κB, while increasing the expression of the CDK inhibitor p21waf1/C1. Taken together, tablysin-15 might inhibit melanoma cell metastasis and proliferation by competing with αvß3 integrin, thereby blocking FAK-associated signaling pathways and nuclear translocation of NF-κB. CONCLUSION: Tablysin-15 has reliable anti-cancer effects against M21 melanoma cells, suggesting tablysin-15 is a promising anti-tumor drug.
Assuntos
Proteínas de Insetos/farmacologia , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Melanoma/tratamento farmacológico , NF-kappa B/metabolismo , Proteínas e Peptídeos Salivares/farmacologia , Neoplasias Cutâneas/tratamento farmacológico , Apoptose/efeitos dos fármacos , Adesão Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Desintegrinas/química , Desintegrinas/farmacologia , MAP Quinases Reguladas por Sinal Extracelular/antagonistas & inibidores , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Quinase 1 de Adesão Focal/antagonistas & inibidores , Quinase 1 de Adesão Focal/metabolismo , Humanos , Integrina alfaVbeta3/antagonistas & inibidores , Integrina alfaVbeta3/metabolismo , Melanoma/metabolismo , Melanoma/patologia , Oligopeptídeos/metabolismo , Fosforilação , Proteínas Proto-Oncogênicas c-akt/metabolismo , Neoplasias Cutâneas/metabolismo , Neoplasias Cutâneas/patologiaRESUMO
Lactococcus petauri CF11 was originally isolated from the gut of healthy humans. To determine the underlying molecular and genetic mechanisms of the probiotic potential of CF11, we performed complete genome sequencing, annotation, and comparative genome analysis. The complete genome of L. petauri CF11 comprised of 1,997,720 bp, with a DNA G+C content of 38.21 mol% containing 1982 protein coding genes and 16 rRNA operons. We found that 1206 genes (56.05%) were assigned a putative function using the gene ontology (GO) resource. The gene products of CF11 were primarily concentrated in molecular function and biological processes, such as catalysis, binding, metabolism, and cellular processes. Furthermore, 1,365 (68.87%) genes were assigned an illative function using COGs. CF11 proteins were associated with carbohydrate transport and metabolism, and amino acid transport and metabolism. This indicates that CF11 bacteria can perform active energy exchange. We classified 1,111 (56.05%) genes into six KEGG functional categories; fructose-bisphosphate aldolase and the phosphoenol pyruvate:phosphotransferase system (PTS), which are necessary in producing short-chain fatty acids (SCFAs), were excited in the carbohydrate metabolic pathway. This suggests that L. petauri CF11 produces SCFAs via glycolysis. The genomic island revealed that some regions contain fragments of antibiotic resistance and bacteriostatic genes. In addition, ANI analysis showed that L. petauri CF11 had the closest relationship with L. petauri 159469T, with an average nucleotide consistency of 98.03%. Taken together, the present study offers further insights into the functional and potential role of L. petauri CF11 in health care.
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The effects of organophosphorus pesticides (OPs), oxons and their ozonation byproducts on gap junctional intercellular communication (GJIC) on cultured BRL cell line were investigated using scrape loading and dye transfer (SL/DT) technique. The neutral red uptake assay was used to identify the non-cytotoxic levels of diazinon, parathion and methyl-parathion applied to GJIC assay. The concentration-dependent inhibition of GJIC was observed over a range of 50-350 mg/l diazinon, parathion and methyl-parathion after 90 min incubation compared with the vehicle control. However, oxons and ozonation byproducts of OPs had no inhibition effect on GJIC at any of the concentrations tested. The inhibition of GJIC by OPs was reversible after removal of the tested pesticides followed by incubation with fresh medium. The present study suggested that the ozonation treatment could be used for the detoxification of drinking water and food crops contaminated with diazinon, parathion and methyl-parathion without formation of GJIC toxicity.
Assuntos
Comunicação Celular/efeitos dos fármacos , Junções Comunicantes/efeitos dos fármacos , Inseticidas/química , Inseticidas/toxicidade , Fígado/citologia , Fígado/efeitos dos fármacos , Compostos Organofosforados/química , Compostos Organofosforados/toxicidade , Ozônio/química , Animais , Linhagem Celular , Diazinon/química , Diazinon/toxicidade , Junções Comunicantes/ultraestrutura , Indicadores e Reagentes , Paraoxon/análogos & derivados , Paraoxon/química , Paraoxon/toxicidade , Paration/química , Paration/toxicidade , Ratos , Ratos Endogâmicos BUFRESUMO
Metastasis is an inherent feature of breast cancer and transient receptor potential (TRP) channels were found to be potentially implicated in this process. Particularly, TRPM7 may regulate cell motility. We therefore examined the expression of TRPM7 mRNA in the Oncomine database and found that TRPM7 is correlated to metastasis and invasive breast cancer. Silencing TRPM7 with RNA interference resulted in a significant decrease in migration and invasion capability of MDA-MB-435 breast cancer cells, and phosphorylation levels of Src and MAPK but not AKT. Our results suggest that TRPM7 regulates migration and invasion of metastatic breast cancer cells via MAPK pathway.
Assuntos
Neoplasias da Mama/patologia , Sistema de Sinalização das MAP Quinases/fisiologia , Canais de Cátion TRPM/fisiologia , Linhagem Celular Tumoral , Movimento Celular , Feminino , Humanos , Invasividade Neoplásica , Fosfatidilinositol 3-Quinases/fisiologia , Proteínas Serina-Treonina Quinases , Proteínas Proto-Oncogênicas c-akt/fisiologia , RNA Interferente Pequeno/genéticaRESUMO
Sufficient lysis of soil or sediment microbes is a critical step for analyzing microbial community structures and for preparing metagenomic DNA libraries. The present study compared lysis methods for recovering archaeal, bacterial, actinomycete, and fungal DNAs from a mangrove sediment sample. PCR results showed that individual procedures using SDS, lysozyme, sonication, freeze-thaw, microwave, and vigorous shaking could extract archaeal or bacterial DNA but failed for actinomycetes or fungi cells. In comparison, an integrated lysis procedure using SDS, lysozyme, and vigorous shaking successfully obtained fungal DNA, and a combination of SDS, lysozyme, vigorous shaking, and microwave treatments recovered DNA from actinomycetes. Denaturing gradient gel electrophoresis (DGGE) results showed that although single lysis procedures can lyse bacterial DNA, all of them assessed the indigenous bacterial community structure with significant biases. The integrated lysis protocols described in the present study could be useful for extracting DNA from various types of sediments.
Assuntos
DNA Arqueal/isolamento & purificação , DNA Bacteriano/isolamento & purificação , DNA Fúngico/isolamento & purificação , Sedimentos Geológicos/microbiologia , Eletroforese em Gel de Gradiente Desnaturante/métodos , Reação em Cadeia da Polimerase , Microbiologia da ÁguaRESUMO
In the present study, organophosphorus pesticides (OPs) (diazinon, methyl parathion, and parathion) were oxidized by bubbling ozone into a glass reactor. OP residues were detected using HPLC and ozonation intermediates were identified using GC-MS. The degradation of OPs followed pseudo-first-order kinetics through direct ozone oxidation and indirect hydroxyl radical oxidation. Diazinon, based on its relatively higher degradation constant, was easily degraded by ozonation. Increasing the pH of the solution accelerated diazinon degradation, but little effect was observed for methyl parathion or parathion. Diazoxon, methyl paraoxon and paraoxon were identified as ozonation intermediates of diazinon, methyl parathion and parathion, respectively. The ozonation of the PS group results in the formation of oxon intermediates, which suggests that OPs with this group would be degraded in a similar manner to that seen for the OPs tested in this study. Diazoxon was completely decomposed by ozonation in 30min, while trace methyl paraoxon and paraoxon accumulated to different amounts when the solution pH was varied. The presence of oxon intermediates should be noted in OP removal by ozonation.