Near-resonant twin-beam generation from degenerate four-wave mixing in hot 133Cs vapor enabled by field-dressed energy levels.

Squeezed light near an atomic resonance is beneficial for efficient atom-light quantum interfaces. It is desirable but challenging to directly generate in atoms due to excess noise from spontaneous emission and reabsorption. Here, we report on the use of energy-level modulation to actively control atomic coherence and interference in degenerate four-wave mixing (DFWM) and then to enhance the DFWM gain process for the generation of near-resonant squeezed twin beams. With this technique, we obtain a -2.6 dB intensity-difference squeezing detuned 100 MHz from the D1 F = 4 to F' = 4 transition of 133Cs.

Propagation of noninteger cylindrical vector vortex beams in a gradient-index fiber.

The characteristics of two noninteger cylindrical vector vortex beams (NCVVBs) propagating through a radial gradient-index (GRIN) fiber are analyzed on the basis of the generalized Huygens-Fresnel principle. The NCVVBs exhibit periodic and stable transmission characteristics in the radial GRIN fiber. Polarization changes, the presence of spin angular momentum (SAM), and changes in the orbital angular momentum (OAM) of the NCVVBs are observed at the focal plane of the radial GRIN fiber. Spin-orbit interactions of NCVVBs are verified in the radial GRIN fiber for the first time, to the best of our knowledge.

Prognostic factors and outcomes of surgical intervention for patients with spinal metastases secondary to lung cancer: an update systematic review and meta analysis.

PURPOSE: Lung cancer is one of the most common malignant tumors. Most patients develop spinal metastases during the course of cancer and suffer skeletal-related events. Currently, no consensus has been reached on the prognostic factors in patients undergoing surgeries. This study aimed to answer two questions: (1) what are the effects of surgical intervention, and (2) what are the factors associated with postoperative survival. METHODS: Searches were performed on electronic databases including PubMed, Ovid/MEDLINE, Cochrane, and Scopus for articles published before February of 2022, involving the survival factors of patients with spinal metastasis. Multiple data items were considered, such as baseline demographics, surgical details, clinical outcome, and prognostic factors. The analysis was performed in Review Manager (RevMan) 5.5. The prognostic factors of survival were analyzed with univariate and multivariate cox regression analysis. RESULTS: Finally, 14 studies with 813 patients were identified. Their 6, 12, and 24 months survival rates ranged from 18 to 58%, 18 to 22.4%, and 0 to 58.5%, respectively. The pooled hazard ratio of preoperative ambulatory status and the number of involved vertebrae demonstrated statistical significance, while no significant prognostic effect on the overall survival was found for targeted therapy, visceral metastases, chemotherapy, radiotherapy, or postoperative ambulatory status. CONCLUSION: Overall, surgical intervention could achieve significant pain relief and neurological function improvements. For patients receiving surgery for spinal metastasis from lung cancer, preoperative ambulatory status and the number of involved vertebrae were significant prognostic factors associated with their survival.

Endogenous FGF21 attenuates blood-brain barrier disruption in penumbra after delayed recanalization in MCAO rats through FGFR1/PI3K/Akt pathway. / å† æºæ€§FGF21通过FGFR1/PI3K/Akt通路减轻MCAOå¤§é¼ å»¶è¿Ÿè¡€ç®¡å†é€šåŽåŠæš—å¸¦çš„è¡€è„‘å±éšœæŸä¼¤ï¼ˆè‹±æ–‡ï¼‰.

OBJECTIVES: Restoration of blood circulation within "time window" is the principal treating goal for treating acute ischemic stroke. Previous studies revealed that delayed recanalization might cause serious ischemia/reperfusion injury. However, plenty of evidences showed delayed recanalization improved neurological outcomes in acute ischemic stroke. This study aims to explore the role of delayed recanalization on blood-brain barrier (BBB) in the penumbra (surrounding ischemic core) and neurological outcomes after middle cerebral artery occlusion (MCAO). METHODS: Recanalization was performed on the 3rd day after MCAO. BBB disruption was tested by Western blotting, Evans blue dye, and immunofluorescence staining. Infarct volume and neurological outcomes were evaluated on the 7th day after MCAO. The expression of fibroblast growth factor 21 (FGF21), fibroblast growth factor receptor 1 (FGFR1), phosphatidylinositol-3-kinase (PI3K), and serine/threonine kinase (Akt) in the penumbra were observed by immunofluorescence staining and/or Western blotting. RESULTS: The extraversion of Evans blue, IgG, and albumin increased surrounding ischemic core after MCAO, but significantly decreased after recanalization. The expression of Claudin-5, Occludin, and zona occludens 1 (ZO-1) decreased surrounding ischemic core after MCAO, but significantly increased after recanalization. Infarct volume reduced and neurological outcomes improved following recanalization (on the 7th day after MCAO). The expressions of Claudin-5, Occludin, and ZO-1 decreased surrounding ischemic core following MCAO, which were up-regulated corresponding to the increases of FGF21, p-FGFR1, PI3K, and p-Akt after recanalization. Intra-cerebroventricular injection of FGFR1 inhibitor SU5402 down-regulated the expression of PI3K, p-Akt, Occludin, Claudin-5, and ZO-1 in the penumbra, which weakened the beneficial effects of recanalization on neurological outcomes after MCAO. CONCLUSIONS: Delayed recanalization on the 3rd day after MCAO increases endogenous FGF21 in the penumbra and activates FGFR1/PI3K/Akt pathway, which attenuates BBB disruption in the penumbra and improves neurobehavior in MCAO rats.

Detecting the transverse spin density of light via electromagnetically induced transparency.

For light that is transversely confined, its field vector spins in a plane not orthogonal to the propagation direction, leading to the presence of transverse spin, which plays a fundamental role in the field of chiral quantum optics. Here, we theoretically propose a scheme to detect the transverse spin density (TSD) of light by utilizing a multilevel atomic medium. The scheme is based on the electromagnetically induced transparency effect, which enables the TSD-dependent modulation of the susceptibility of the atomic medium by using a coupling field whose TSD is to be detected. The modulated susceptibility results in a spin-dependent absorption for a probe beam passing through the atomic medium. We show that there exists a corresponding relationship between the TSD distribution of the coupling field and the polarization distribution of the transmitted probe beam through a theoretical study of two typical cases, in which the coupling field is provided by a tightly focused field and a two-beam interference field, respectively. Based on this relationship, the key features of the TSD of the coupling field, such as the spatial distribution, the symmetry property, and the spin-momentum locking, can be inferred from the transmitted probe beam. Benefiting from the fast response of the atomic medium to the variation of the coupling field, the present scheme is capable of detecting the TSD in real time, offering new possibilities for developing transverse-spin-based techniques.

Experimental realization of efficient nondegenerate four-wave mixing in cesium atoms.

Nondegenerate four-wave mixing (FWM) in diamond-type atomic systems has important applications in a wide range of fields, including quantum entanglement generation, frequency conversion, and optical information processing. Although the efficient self-seeded nondegenerate FWM with amplified spontaneous emission (ASE) has been realized extensively, the seeded nondegenerate FWM without ASE is inefficient in reported experiments so far. Here we present the experimental realization of the seeded nondegenerate FWM in cesium atoms with a significantly improved efficiency. Specifically, with two pump lasers at 852 and 921 nm and a seed laser at 895 nm, a continuous-wave laser at 876 nm is efficiently generated via FWM in a cesium vapor cell with a power up to 1.2 mW, three orders of magnitude larger than what has been achieved in previous experiments. The improvement of the efficiency benefits from the exact satisfaction of the phase-matching condition realized by an elaborately designed setup. Our results may find applications in the generation of squeezing and entanglement of light via nondegenerate FWM.

Propagation of Gaussian vortex beams in electromagnetically induced transparency media.

Electromagnetically induced transparency (EIT) is an important phenomenon in quantum optics, and has a wide range of applications in the fields of quantum information processing and quantum precision metrology. Recently, with the rapid progress of the generation and detection of structured light, the EIT with structured light has attracted enormous interests and offers new and novel functionalities and applications. Here, we theoretically study the propagation and evolution of Gaussian vortex beams, a typical type of structured light, in an EIT medium with Λ-type three-level atoms. Based on the generalized Huygens-Fresnel principle, we derive the analytical expressions of fully and partially coherent Gaussian vortex beams propagating in the EIT medium, and study the evolution of the intensity and phase distributions of the beams and their dependencies on parameters such topological charge, coherence length, Rabi frequency, etc. It is shown that both the fully and partially coherent Gaussian vortex beams undergo focusing and diverging periodically during propagation. The phase singularity of the fully coherent beam keeps unchanged, while the phase singularity of the partially coherent beam experiences splitting and recombination periodically. In addition, new phase singularities with opposite topological charge are generated in the latter case. Our results not only advance the study of the interaction between structured light and coherent media, but also pave the avenue for manipulating structured light via EIT.

Effect of Regional vs General Anesthesia on Incidence of Postoperative Delirium in Older Patients Undergoing Hip Fracture Surgery: The RAGA Randomized Trial.

Importance: In adults undergoing hip fracture surgery, regional anesthesia may reduce postoperative delirium, but there is uncertainty about its effectiveness. Objective: To investigate, in older adults undergoing surgical repair for hip fracture, the effects of regional anesthesia on the incidence of postoperative delirium compared with general anesthesia. Design, Setting, and Participants: A randomized, allocation-concealed, open-label, multicenter clinical trial of 950 patients, aged 65 years and older, with or without preexisting dementia, and a fragility hip fracture requiring surgical repair from 9 university teaching hospitals in Southeastern China. Participants were enrolled between October 2014 and September 2018; 30-day follow-up ended November 2018. Interventions: Patients were randomized to receive either regional anesthesia (spinal, epidural, or both techniques combined with no sedation; n = 476) or general anesthesia (intravenous, inhalational, or combined anesthetic agents; n = 474). Main Outcomes and Measures: Primary outcome was incidence of delirium during the first 7 postoperative days. Secondary outcomes analyzed in this article include delirium severity, duration, and subtype; postoperative pain score; length of hospitalization; 30-day all-cause mortality; and complications. Results: Among 950 randomized patients (mean age, 76.5 years; 247 [26.8%] male), 941 were evaluable for the primary outcome (6 canceled surgery and 3 withdrew consent). Postoperative delirium occurred in 29 (6.2%) in the regional anesthesia group vs 24 (5.1%) in the general anesthesia group (unadjusted risk difference [RD], 1.1%; 95% CI, -1.7% to 3.8%; P = .48; unadjusted relative risk [RR], 1.2 [95% CI, 0.7 to 2.0]; P = .57]). Mean severity score of delirium was 23.0 vs 24.1, respectively (unadjusted difference, -1.1; 95% CI, -4.6 to 3.1). A single delirium episode occurred in 16 (3.4%) vs 10 (2.1%) (unadjusted RD, 1.1%; 95% CI, -1.7% to 3.9%; RR, 1.6 [95% CI, 0.7 to 3.5]). Hypoactive subtype in 11 (37.9%) vs 5 (20.8%) (RD, 11.5; 95% CI, -11.0% to 35.7%; RR, 2.2 [95% CI, 0.8 to 6.3]). Median worst pain score was 0 (IQR, 0 to 20) vs 0 (IQR, 0 to 10) (difference 0; 95% CI, 0 to 0). Median length of hospitalization was 7 days (IQR, 5 to 10) vs 7 days (IQR, 6 to 10) (difference 0; 95% CI, 0 to 0). Death occurred in 8 (1.7%) vs 4 (0.9%) (unadjusted RD, -0.8%; 95% CI, -2.2% to 0.7%; RR, 2.0 [95% CI, 0.6 to 6.5]). Adverse events were reported in 106 episodes in the regional anesthesia group and 102 in the general anesthesia group; the most frequently reported adverse events were nausea and vomiting (47 [44.3%] vs 34 [33.3%]) and postoperative hypotension (13 [12.3%] vs 10 [9.8%]). Conclusions and Relevance: In patients aged 65 years and older undergoing hip fracture surgery, regional anesthesia without sedation did not significantly reduce the incidence of postoperative delirium compared with general anesthesia. Trial Registration: ClinicalTrials.gov Identifier: NCT02213380.

Experimental realization of wavelength multiplexed nonlinear upconversion in cesium atoms.

Blue-violet wavelength multiplexing offers the capability to enhance the operational capacity and efficiency in optical information processing, underwater communications, biology, and medicine. Here we present a specially designed atomic scheme of cesium for simultaneously generating two blue-violet lasers at 456 and 459 nm via four-wave-mixing (FWM)-based upconversion processes. We experimentally and theoretically show that a double-diamond-type coherent system pumped with 852 and 921 nm lasers is responsible for the efficient generation of the two blue-violet lasers. In this system, the large population inversions lead to self-amplified spontaneous emissions at 15.6 and 12.1µm, which, in turn, serve as the seeds for enhanced double FWM processes with dual-wavelength upconversions.

Observation of the interplay between seeded and self-seeded nondegenerate four-wave mixing in cesium vapor.

Nondegenerate four-wave mixing (NFWM) is a practical and effective technique for generating or amplifying light fields at different wavelengths, and could be used to create color correlation and entanglement. Here we experimentally investigate the NFWM process in diamond atomic system via two-photon excitation with two pumps at 852 nm and 921 nm, demonstrating that a seeded NFWM with a third laser at 895 nm and two self-seeded NFWMs due to amplified spontaneous emission (ASE) occur simultaneously. We compare the two kinds of processes and show that the single- and two-photon detunings hold the key role in distinguishing them. As a result, the enhancement of seeded NFWM is obtained by selecting large one- and two-photon detunings, in which case the ASE induced self-seeded NFWM can be largely suppressed. In contrast, the ASE and its induced NFWM are effectively achieved with one- and two-photon resonant excitations allowing for population inversion for efficient ASE.

Experimental Observation of Momentum-Space Chiral Edge Currents in Room-Temperature Atoms.

Chiral edge currents play an important role in characterizing topological matter. In atoms, they have been observed at such a low temperature that the atomic motion can be measured. Here we report the first experimental observation of chiral edge currents in atoms at room temperature. Staggered magnetic fluxes are induced by the spatial phase difference between two standing-wave light fields, which couple atoms to form a momentum-space zigzag superradiance lattice. The chiral edge currents are measured by comparing the directional superradiant emissions of two timed Dicke states in the lattice. Our results pave the way for simulating topological physics in hot atoms.

Fast Lithium-Ion Transportation in Crystalline Polymer Electrolytes.

Fast lithium-ion transportation is found in the crystalline polymer electrolytes, α-CD-PEOn /Li+ (n=12, 40), prepared by self-assembly of α-cyclodextrin (CD), polyethylene oxide (PEO) and Li+ salts. A detailed solid-state NMR study combined with the X-ray diffraction technique reveals the unique structural features of the samples, that is, a) the tunnel structure formed by the assembled CDs, providing the ordered long-range pathway for Li+ ion transportation; b) the all-trans conformational sequence of the PEO chains in the tunnels, attenuating significantly the coordination between Li+ and the EO segments. The origin of the fast lithium-ion transportation has been attributed to these unique structural features. This work demonstrates the first example in solid polymer electrolytes (SPEs) for "creating" fast ion transportation through material design and will find potential applications in the design of new ionconducting SPE materials.

Cathepsin L promotes Vascular Intimal Hyperplasia after Arterial Injury.

The inflammatory pathways that drive the development of intimal hyperplasia (IH) following arterial injury are not fully understood. We hypothesized that the lysosomal cysteine protease cathepsin L activates processes leading to IH after arterial injury. Using a mouse model of wire-induced carotid artery injury we showed that cathepsin L activity peaks at day 7 and remains elevated to 28 days. The genetic deletion of cathepsin L prevented IH and monocyte recruitment in the carotid wall. The injury-induced increases in cathepsin L mRNA and activity were mitigated in mice with myeloid-specific deletion of toll like receptor 4 (TLR4) or myeloid differentiation primary response gene 88 (MyD88). We further discovered that a HIV-protease inhibitor saquinavir (SQV), which is known to block recombinant mouse cathepsin L activity in vitro, prevented IH after arterial injury. SQV also suppressed LPS (TLR4 agonist) induced monocyte adhesion to endothelial monolayers. These findings establish cathepsin L as a critical regulator of the inflammation that leads to IH and that the TLR4- MyD88 pathway in myeloid lineages regulates cathepsin L expression in the vessel wall following wire injury. The FDA approved drug, SQV blocks IH though mechanisms that may include the suppression of cathepsin L.

Single-frequency tunable 447.3 nm laser by frequency doubling of tapered amplified diode laser at cesium D1 line.

A continuous single-frequency tunable blue laser at 447.3 nm is developed by external-cavity frequency doubling of a tapered amplifier-boosted continuous-wave diode laser at cesium (Cs) D1 line. A maximum blue power of 178 mW with 50.8% conversion efficiency is obtained. It can be continuously tuned over a range around 1.6 GHz as the diode laser frequency is scanned across the F=4→F'=3 transition of 133Cs D1 line. The generated tunable and stable blue laser source has potential applications in constructing quantum light-atom interfaces in quantum networks.

HMGB1-Driven Inflammation and Intimal Hyperplasia After Arterial Injury Involves Cell-Specific Actions Mediated by TLR4.

OBJECTIVE: Endoluminal vascular interventions such as angioplasty initiate a sterile inflammatory response resulting from local tissue damage. This response drives the development of intimal hyperplasia (IH) that, in turn, can lead to arterial occlusion. We hypothesized that the ubiquitous nuclear protein and damage-associated molecular pattern molecule, high-mobility group box 1 (HMGB1), is one of the endogenous mediators that activates processes leading to IH after endoluminal injury to the arterial wall. The aim of this study is to investigate whether approaches that reduce the levels of HMGB1 or inhibit its activity suppresses IH after arterial injury. APPROACH AND RESULTS: Here, we show that HMGB1 regulates IH in a mouse carotid wire injury model. Induced genetic deletion or neutralization of HMGB1 prevents IH, monocyte recruitment, and smooth muscle cell growth factor production after endoluminal carotid artery injury. A specific inhibitor of HMGB1 myeloid differentiation factor 2-toll-like receptor 4 (TLR4) interaction, P5779, also significantly inhibits IH. HMGB1 deletion is mimicked in this model by global deletion of TLR4 and partially replicated by myeloid-specific deletion of TLR4 but not TLR2 or receptor for advanced glycation endproducts deletion. The specific HMGB1 isoform known to activate TLR4 signaling (disulfide HMGB1) stimulates smooth muscle cell to migrate and produce monocyte chemotactic protein 1/CCL2) via TLR4. Macrophages produce smooth muscle cell mitogens in response to disulfide HMGB1 also in a TLR4/myeloid differentiation primary response gene (88)/Trif-dependent manner. CONCLUSIONS: These findings place HMGB1 and its receptor, TLR4 as critical regulators of the events that drive the inflammation leading to IH after endoluminal arterial injury and identify this pathway as a possible therapeutic target to limit IH to attenuate damage-associated molecular pattern molecule-mediated vascular inflammatory responses.

Inhibition of PKR impairs angiogenesis through a VEGF pathway.

Peripheral artery disease (PAD) is a common clinical problem, and its pathophysiological mechanisms are incompletely understood. Double-stranded RNA-activated protein kinase (PKR) is a ubiquitously expressed serine/threonine protein kinase. Although PKR has been reported in antivirus and the immune system, the role of PKR in vascular function, especially in angiogenesis, is still unclear. PKR(-/-) mice were used in our experiments. Blood flow recovery was significantly delayed in PKR(-/-) vs. WT mice (Laser Doppler detection, n = 9, P < 0.01), accompanied by 34% reduced CD31-positive stain in ischemic muscle 28 days after procedure (immunohistochemistry, n = 9, P < 0.05). PKR expression decreased in the first 12 h and increased to peak at 24 h in human umbilical vein endothelial cells (HUVECs) in response to hypoxia (Western blot analyses, n = 3, P < 0.05). Accordingly, phospho-PKR expression increased in HUVECs 24 h after treatment with hypoxia (Western blot analyses, n = 3, P < 0.05). Inhibition of PKR (siRNA transfection) reduced microtubule formation (Matrigel tube formation, n = 3, vs. control siRNA, P < 0.05) and migration (wound healing, n = 3, vs. control siRNA, P < 0.05) by 33 and 59%, respectively. Vascular endothelial growth factor (VEGF) expression in ischemic muscle from PKR(-/-) mice was significantly decreased by 54% 1 day after procedure (n = 3, P < 0.05, vs. WT) and by 63% 7 days after procedure (n = 3, P < 0.01, vs. WT), respectively. At the same time, VEGF expression in HUVECs decreased by 21% (n = 3, P < 0.05, PKR siRNA vs. control siRNA). These findings demonstrate that PKR mediates angiogenesis through a VEGF pathway, which may form the basis for future intervention of PAD.

Association between weekend catch-up sleep and depression of the United States population from 2017 to 2018: A cross-sectional study.

Insufficient sleep on weekdays has become a societal norm, and studies have shown that sleep deprivation increases the risk of depression. Although individuals often resort to weekend catch-up sleep (CUS) as a compensatory measure, the present evidence supporting its efficacy in mitigating the risk of depression is limited. This article attempts to explore the relationship between CUS and depression. In this study, a total of 5510 participants were included, characterized into two groups: nondepressed (n = 5051) and depressed (n = 459), with data extracted from the National Health and Nutrition Examination Survey (NHANES). Compared with people without CUS, those practicing CUS exhibited a significantly lower risk of depression (OR = 0.81, P = 0.048). In subgroup analysis, this reduction effect was only observed in males (OR = 0.70, 95 % CI 0.05 to 0.99, P = 0.04), middle-aged (>40, ≤60) (OR: 0.57, 95 % CI: 0.40 to 0.81, P = 0.002), married or living with parents (OR: 0.61, 95 % CI: 0.44 to 0.86, P = 0.004), groups with three or more family members (OR: 0.69, 95 % CI: 0.52 to 0.93, P = 0.01), and individuals without alcohol intake (OR: 0.24,95 % CI: 0.09 to 0.67, P = 0.006). Therefore, in the realm of depression treatment, doctors may consider advising patients to get adequate sleep on weekends as part of their overall treatment plan. At the same time, individuals can also choose weekend sleep as a proactive strategy for regulating their psychological status.

Engineering the NASICON electrolyte/Na anode interface with amorphous bismuth oxide for sodium batteries.

Amorphous BiOx, prepared on the surface of NASICON electrolyte by the photochemical metal-organic deposition method, can substantially improve the interfacial properties at the anode side. The Na symmetric cell delivers a critical current density of 1.2 mA cm-2 and cycles stably at 0.5 mA cm-2 for 1000 h at 30 °C.

Neuroinflammation in epileptogenesis: from pathophysiology to therapeutic strategies.

Epilepsy is a group of enduring neurological disorder characterized by spontaneous and recurrent seizures with heterogeneous etiology, clinical expression, severity, and prognosis. Growing body of research investigates that epileptic seizures are originated from neuronal synchronized and excessive electrical activity. However, the underlying molecular mechanisms of epileptogenesis have not yet been fully elucidated and 30% of epileptic patients still are resistant to the currently available pharmacological treatments with recurrent seizures throughout life. Over the past two decades years accumulated evidences provide strong support to the hypothesis that neuroinflammation, including microglia and astrocytes activation, a cascade of inflammatory mediator releasing, and peripheral immune cells infiltration from blood into brain, is associated with epileptogenesis. Meanwhile, an increasing body of preclinical researches reveal that the anti-inflammatory therapeutics targeting crucial inflammatory components are effective and promising in the treatment of epilepsy. The aim of the present study is to highlight the current understanding of the potential neuroinflammatory mechanisms in epileptogenesis and the potential therapeutic targets against epileptic seizures.

Mannose inhibits proliferation and promotes apoptosis to enhance sensitivity of glioma cells to temozolomide through Wnt/ß-catenin signaling pathway.

BACKGROUND: Temozolomide (TMZ) is generally applied for glioma treatment, while drug resistance of TMZ limits its therapeutic efficacy. Mannose exerts evident anti-tumor effect. We intended to investigate whether mannose enhanced TMZ sensitivity to glioma and examined the underlying mechanism. METHODS: MTT and clone formation assays were performed to detect cell viability and proliferation. Cell apoptosis was measured by flow cytometry. The protein and gene expression levels were detected by Western blot and qRT-PCR assays. Xenograft glioma model was established to explore the influence of mannose in vivo. RESULTS: Mannose inhibited glioma cell growth, which was facilitated by knockdown of phosphomannose isomerase (PMI) while reversed by overexpression of PMI. Mannose enhanced the sensitivity of glioma cells to TMZ, indicated by the further inhibited cell viability and colony formation and the aggravated cell apoptosis, which was reversed by overexpression of O6-methylguanine DNA methyltransferase (MGMT). Furthermore, mannose and TMZ inhibited MGMT expression and Wnt/ß-catenin activation. Moreover, activating Wnt/ß-catenin pathway blocked anti-proliferative effect induced by mannose and TMZ, which was further suppressed by overexpressed MGMT. Mannose inhibited glioma growth, suppressed Ki67 and downregulated MGMT and ß-catenin in vivo. CONCLUSION: Mannose inhibited MGMT to enhance sensitivity of glioma cells to TMZ, with Wnt/ß-catenin pathway involvement. Our data suggested that mannose could be an innovative agent to improve glioma treatment, particularly in TMZ-resistant glioma with high MGMT.