Cadmium threshold for acidic and multi-metal contaminated soil according to Oryza sativa L. Cadmium accumulation: Influential factors and prediction model.

Cadmium (Cd) contamination in soil-rice systems has become a global public concern. However, influencing factors and the contamination threshold of Cd in soils remain largely unknown owing to soil heterogeneity, which limits our ability to assess the risk to human health and to draft appropriate environmental policies. In this study, we selected the soil-rice system of Longtang and Shijiao town in southern China, which was characterized by multi-metal acidic soil contamination due to improper electronic waste recycling activities, as a case to analyze the influence of different soil properties on the Cd threshold in the soil and Cd accumulation in rice. The results showed that soil organic matter (SOM) was the main factor regulating Cd accumulation in the soil-rice system. Moreover, compared with the total Cd concentration, the DTPA-extractable Cd concentration in the soil was a better predictor of Cd transportation in the soil-rice system. According to the prediction model, when SOM was < 35 g kg-1, the CdDTPA threshold was 0.16 mg kg-1 with a 95% likelihood of Cdrice accumulation above the Chinese food standard limit (0.2 mg kg-1). Conversely, when SOM was ≥ 35 g kg-1, the CdDTPA threshold was only 0.03 mg kg-1. This study of the influence of SOM on Cd accumulation in a soil-rice system confirms that SOM is a crucial parameter for better and safer rice production, especially in multi-metal contaminated acidic soils.

Application of phosphorus amendments reduces metal uptake and increases yield of Oryza saliva L. (rice) in Cd/Cu-contaminated paddy field.

To alleviate worldwide food safety issues caused by metal contamination, an easily available material is urgently needed for extensive application. In this study, calcium magnesium phosphate fertiliser (Pcm) was applied to a Cd/Cu co-contaminated paddy field in comparison with limestone and organic fertiliser. The results showed that only Pcm is effective in simultaneously reducing Cd uptake by 56.7% and Cu uptake by 36.2% in Oryza saliva L. (rice). The rice yield, reduced mainly by Cu, also increased by 30.1% with respect to the enhancement of soil pH, cation exchange capacity and availability of phosphorus, as well as the reduction in availabilities of Cd and Cu. Additionally, Pcm dramatically shaped the bacterial community structure, with Proteobacteria and Firmicutes predominant in the soils. The beneficial genera Exiguobacterium, Citrobacter, and Acinetobacter, which are vital for phosphate dissolution and Cd/Cu immobilisation, were also enriched. The results demonstrated that the application of Pcm at 0.4% (w:w) was able to enhance both crop quantity and quality in Cd/Cu co-contaminated paddy fields by reducing Cu/Cd availability, promoting rice yield, and reshaping bacterial community structures.

Groundwater contaminated with short-chain chlorinated paraffins and microbial responses.

The vertical migrations of toxic and persistent short-chain chlorinated paraffins (SCCPs) in soils as well as the microbial responses have been reported, however, there is a paucity of data on the resulting groundwater contamination. Here, we determined the concentration and congener profile of SCCPs in the groundwater beneath a production plant of chlorinated paraffins (CPs) and characterized the microbial community to explore their responses to SCCPs. Results showed that SCCPs ranged from not detected to 70.3 µg/L, with C13-CPs (11.2-65.8%) and Cl7-CPs (27.2-50.6%), in mass ratio, as the dominant groups. Similar to the distribution pattern in soils, SCCPs in groundwater were distributed in hotspot pattern. CP synthesis was the source of SCCPs in groundwater and the entire contamination plume significantly migrated downgradient, while there was an apparent hysteresis of C13-CP migration. Groundwater microbial community was likely shaped by both hydrogeological condition (pH and depth) and SCCPs. Specifically, the microbial community responded to the contamination by forming a co-occurrence network with "small world" feature, where Desulfobacca, Desulfomonile, Ferritrophicum, Methylomonas, Syntrophobacter, Syntrophorhabdus, Syntrophus, and Thermoanaerobaculum were the keystone taxa. Furthermore, the interrelations between bacterial taxa and SCCPs indicated that the microbial community might cooperate to achieve the dechlorination and mineralization of SCCPs through either anaerobic organohalide respiration mainly functioned by the keystone taxa, or cometabolic degradation processes functioned by Aquabacterium and Hydrogenophaga. Results of this study would provide a better understanding of the environmental behavior and ecological effects of SCCPs in groundwater systems.

Mitigation of Cd accumulation in paddy rice (Oryza sativa L.) by Fe fertilization.

Cadmium uptake in rice is believed to be mediated by the Fe transport system. Phyto-available Cd can be changed by Fe fertilization of substrates. This work investigated whether and how Fe fertilization affects mitigation of Cd accumulation in paddy rice. A 90-d soil column experiment was conducted to study the change of Cd and Fe availability in soil after Fe fertilization (ionic and chelated Fe). A low-Cd accumulating cultivar (TY116) and a high-Cd accumulating cultivar (JY841) were grown in two Cd-polluted paddy soils amended with chelated Fe fertilizers. Additionally, both cultivars were grown in hydroponics to compare Fe-related gene expression in EDDHAFe-deficient and EDDHAFe-sufficient roots. The column experiment showed that EDTANa2Fe(II) and EDDHAFe(III) fertilization had a better mitigation effect on soil Cd availability compared to FeSO4·7H2O. Moreover, the field experiment demonstrated that these two chelated fertilizations could reduce Cd concentrations in brown rice by up to 80%. Iron concentrations in the brown rice were elevated by Fe chelates. Compared to EDDHAFe(III), EDTANa2Fe(II) fertilization had a stronger mitigation effect by generating more EDTANa2Cd(II) in the soil solution to decrease phyto-available Cd in the soil. While EDDHAFe(III) fertilization could increase soil pH and decrease soil Eh which contributed to decreasing phyto-available Cd in a contaminated soil. In the hydroponic experiment, Fe sufficiency significantly reduced Cd concentrations in above-ground organs. In some cases, the expression of OsIRT1, OsNRAMP1 and OsNRAMP5 was inhibited under Fe sufficiency relative to Fe deficiency conditions. These results suggest that mitigation of rice Cd by Fe chelate fertilization results from a decrease in available Cd in substrates and the inhibition of the expression of several Fe-related genes in the IRT and NRAMP families.