RESUMO
Based on UV spectrum-guided purification, new monordens C, D and E, known monordens A (radicicol) and B and 5-O-methylsclerone were isolated from the fermentation broth of amidepsine-producing Humicola sp. FO-2942 by solvent extraction, silica-gel column chromatography, ODS column chromatography and HPLC. All monordens cause the cell cycle arrest at G1 and G2/M phases in Jurkat cells. But among them, monordens A and E show antifungal activity only against Aspergillus niger.
Assuntos
Antifúngicos , Aspergillus niger/efeitos dos fármacos , Antifúngicos/isolamento & purificação , Antifúngicos/farmacologia , Ciclo Celular/efeitos dos fármacos , Células Cultivadas , Fermentação , Relação Estrutura-AtividadeRESUMO
Structures of three novel compounds designated monordens C to E, isolated from the fermentation broth of amidepsine-producing fungus Humicola sp. FO-2942, were elucidated by spectroscopic evidence. Monordens C is 6,7-dihydromonorden A. Monordens D and E lack the epoxide moiety of monordens C and B, respectively.
Assuntos
Antifúngicos/química , Antifúngicos/isolamento & purificação , Fermentação , Relação Estrutura-AtividadeRESUMO
An incorporation study of [1-(13)C] and [1,2-(13)C2] labeled sodium acetates into sorbicillinol 1 established a ring closure system between C-1 and C-6 and the positions that were oxidized and/or methylated on a hexaketide chain. Subsequent investigations, using 13C-labeled 1 prepared from [1-(13)C] labeled sodium acetate, clearly demonstrated that both bisorbicillinol 2 and sorbicillin 6 incorporated 13C-labeled 1 into their carbon skeletons. 13C-labeled bisorbicillinols 2 derived from [1-(13)C]- and [2-(13)C]-labeled sodium acetates clearly indicate that these were on the biosynthetic route from 1 to bisorbibutenolide (bislongiquinolide) 3 and bisorbicillinolide 4 via 2 as a branching point in the fungus.