RESUMO
BACKGROUND: Carboxymethylpachymaran (CMP) is created by carboxymethylating pachyman (PM), which increases its water solubility and enhances a number of biological activities. Traditional polysaccharides modified by carboxymethylation employ strong chemical techniques. Carboxymethylcellulose (CMC) has been used previously for liquid fermentation to carboxymethyl modify bacterial polysaccharides. This theory can be applied to fungal polysaccharides because Poria cocos has the ability to naturally utilize cellulose. RESULTS: CMC with different degrees of substitution (DS) (0.7, 0.9 and 1.2) were added to P. cocos fermentation medium, and CMPs with different DS (0.38, 0.56 and 0.78, respectively) were prepared by liquid fermentation. The physical and chemical properties and biological activities of the CMPs were determined. Their structures were confirmed by Fourier transform infrared (FTIR) spectroscopy and monosaccharide composition. With the increase of DS, the viscosity and viscosity-average molecular weight of CMPs decreased, whereas polysaccharide content and water solubility increased, although the triple helix structure was not affected. The results of bioactivity assay showed that the higher the DS of CMPs, the higher the 2,2-diphenyl-1-picrylhydrazyl radical scavenging ability, and the stronger the bacterial inhibition ability. CONCLUSION: The present study has developed a method for producing CMPs by P. cocos liquid fermentation. The results of the study confirm that enhancing the DS of CMP could effectively enhance its potential biological activity. The findings provide safe and reliable raw materials for creating CMP-related foods and encourage CMP application in the functional food industry. © 2024 Society of Chemical Industry.
Assuntos
Glucanos , Polissacarídeos , Água , Fermentação , Polissacarídeos/química , Solubilidade , Espectroscopia de Infravermelho com Transformada de Fourier , Água/químicaRESUMO
This work aimed to explore the effects of heat stress and the addition of different purines (adenine, guanine, xanthine, and hypoxanthine) on the synthesis of eritadenine in liquid fermentation of Lentinula edodes. The L. edodes biomass and eritadenine content were determined. The results showed that eritadenine mainly existed in the fermentation broth, and the synthesis of eritadenine might be partially coupled with L. edodes mycelium growth in this study. Heat stress inhibited mycelium growth and the synthesis of eritadenine. The addition of adenine had no effect on the synthesis of eritadenine. However, the addition of guanine and xanthine inhibited the synthesis of eritadenine. When subjected to hypoxanthine, a significant increase in eritadenine production was observed, which was 1.54 times that of the control group.