A modified MBR system with post advanced purification for domestic water supply system in 180-day CELSS: Construction, pollutant removal and water allocation.

Water supply was vital to people's life, especially inside Controlled Ecological Life Support System (CELSS) for long-term space exploration. A platform of 4-person-180-day integrated experiment inside a CELSS including 6 cabins called 'SPACEnter' was established in Shenzhen, China. Based on this platform, a Membrane Bio-Reactor (MBR) system configuring post advanced purification, including I-MBR, II-MBR, nanofiltration (NF), reverse osmosis (RO), ion-exchange (IE), polyiodide disinfection (PI) and mineralization (MC) stages, used as a Domestic Water Supply System (DWSS) to guarantee crew's daily life was constructed. The performance of DWSS to treat the real plant cabin's condensate water was examined during continuously 180-day experiment. The long-term operation results showed that, though the influent pollutant load changed as the experiment processing, the system exhibited stable performance on pollutants removal with average effluent TOC＜0.5 mg/L, NH4+-N＜0.02 mg/L, NO3--N＜0.25 mg/L, NO2--N＜0.001 mg/L, and displayed good capacity for controlling the trace metal ions and microorganism. The effluent through such modified MBR system was sufficiently allocated as hygiene water and potable water, and the average value was 39.69 and 10.93 L/d, respectively. The consumption of the modified MBR process was within the designed allowable scope. The outcomes of this study will be helpful for facilitating future applications of MBR as bio-based water supply technology in the CELSS.

Anaerobic membrane bioreactor for hygiene wastewater treatment in controlled ecological life support systems: Degradation of surfactants and microbial community succession.

The treatment and reuse of hygiene wastewater is crucial to "close the loop" in the controlled ecological life support system (CELSS), and to guarantee longer space missions or planetary habitation. In this work, anaerobic membrane bioreactor (AnMBR) was applied for hygiene wastewater treatment, focused on surfactant degradation and microbial community succession. The removal efficiency of COD and surfactants was 90%∼97% and 80% with a urine source-separation strategy. The microbial community gradually shifted from methanogens to sulfur-metabolizing and surfactant-degradation bacteria, such as Aeromonas. Sulfate was a surfactant degradation product, which triggered sulfate reduction and methane inhibition. The activated carbohydrate and sulfur metabolism were the key mechanism of the microbial process for the excellent performance of AnMBR. This study analyzed the degradation mechanism from the perspective of microbial mechanism, offers a solution for CELSS hygiene wastewater treatment, and supports the future improvement and refinement of AnMBR technology.

Operation overview of a biological waste treatment system during the 4-crew 180-day integrated experiment in the controlled ecological life support system (CELSS).

Waste management and treatment is vital to health care and material circulation, especially in the Controlled Ecological Life Support System (CELSS) with finite resources for long-duration manned space missions. A closed ecological-cycle integrated 4-crew 180-day experiment platform was established to investigate the key technologies such as effective cultivation of higher plant, water treatment and recycling, waste management and treatment. In this study, generated waste during the integrated experiment was classified as renewable and non-renewable waste. The renewable waste including all crew feces and part of inedible plant biomass were treated in a biological system where the aerobic composting technology was utilized. The performance in relation to degradation effect, phytotoxicity and nutrient evaluation was examined during the continuous 180 days. The long-term operation results displayed that 96.26 kg feces and 74.4 kg wheat straw were treated, and 90.6 kg compost product was discharged in nine batches. The microbial community variation was analyzed and Firmicutes, Actinobacteria and Proteobacteria enriched in the compost. The phytotoxicity of compost was examined by seed germination index (GI) and GI of Chinese cabbage ranged from 88% to 132% for all batches. Compared to grown in vermiculite only, the lettuce yield increased 19% when grown in a mixture of vermiculite and processed compost. The summary of this work will be helpful to facilitate future applications of aerobic composting technology as the bio-based waste treatment technology in CELSS.

Design and establishment of a large-scale controlled ecological life-support system integrated experimental platform.

A Controlled Ecological Life-Support System (CELSS) can meet the demands of food, oxygen, and water for human, as well as providing psychological benefits during deep space exploration by the continuous materials regeneration. Many key techniques of the platform are needed to explore before applying to the extraterrestrial planets. In this study, a large-scale CELSS integrated experimental platform was designed and constructed to meet the basic life-support material demands of six crew members (max). The platform was composed of four kinds of cabins including Crew Cabin (CC), Plant Cabin (PC), Life-Support Cabin (LSC), Resource Recycling Cabin (RRC) and affiliated facilities. Eight cabins were involved in the platform, i.e., CCs I and II, PCs I, II, III and IV, LSC, and RRC. The platform involved 15 subsystems and covered a plant culture area of 206.6 m2 (a max extensible area of 260 m2) and a total volume of 1340 m3. The joint debuggings and the 4-subject 180-day CELSS integration experiment were carried out successfully. The material closures were 55% (on average) for food (70.8% in highly efficient production period), 100% for atmospheric regeneration, 100% for water regeneration, and 87.7% for recycled solid waste in the 4-subject 180-day integration experiment. It verified that the indicators of the platform meet the technical requirements and realize food regeneration, air regeneration and water regeneration through the integration of physico-chemical technique and biological technique for the long-term survivals of six crew members in the closed cabins.

Wheat Cultivation and Nutrient Control for the 180-day CELSS Integrated Experiment.

This research aimed to select the well-adapted wheat cultivar and to explore an optimum nutrient control pattern for wheat cultivation in the 180-day integrated experiment of controlled ecological life support system (CELSS). In the experiment, six wheat cultivars from different areas of China were preselected and cultivated in four separate recirculating hydroponic systems (HySy), nutrients in which could be controlled and recycled according the values of pH, electrical conductivity (EC) and dissolved oxygen (DO). Wheat covered an area of 111.3 m2 and had been planted in 17 batches with a 15-day time interval to realize stable regeneration of oxygen, water and food during the 180-day duration in the closed cabin. The results indicated that different cultivars displayed different adaptabilities to the controlled environment. Wt04 had a stronger adaptability with the highest yield (12.82 g DM m-2 d-1) and edible radiation use efficiency (RUE) (0.28 g DM mol-1) whereas Wt06 adapted this environment poorly because of its excessive vegetative growth. For the morphological characters, wheat plants tended to dwarf in the CELSS environment compared with the field. An innovative controlling pattern was established for nutrient supplement. Through the real-time monitoring of pH, EC and DO of the nutrient solution and the periodical detection of the contents of nutrient elements, the nutrient solution could be controlled and recycled continuously without being renewed under a suitable state for wheat plants growth during the 180-day integrated experiment.

Water management in a controlled ecological life support system during a 4-person-180-day integrated experiment: Configuration and performance.

Water management subsystem (WMS) is a major component of the controlled ecological life support system (CELSS). For guaranteeing the water requirement of crop growth and crewmember's daily life, a WMS was established in a 4 person 180-day integrated experiment (carried out in Shenzhen, China, 2016) to maintain a closed cycle with a total water amount of ~23 m3. The design and operation of the WMS was summarized as follows: (1) Collection and allocation of condensate water. About 917 L/d condensate water (>98% was from plants' evapotranspiration) was collected, and ~866 L/d of which was reused as plant nutrient solution after ultraviolet (UV) disinfection, and 50.6 L/d was used as the raw water for the domestic water supply module (DWS). (2) Domestic water supply. The condensate water from the plant cabin was purified through the DWS, a modified membrane bioreactor (MBR) system, and then provided hygiene and potable water to 4 crewmembers with different water quality standards. (3) Wastewater recovery. 51.4 L/d wastewater from urination and personal hygiene were treated together via a biological wastewater treatment process to complete the conversion of nitrogen and organic matters, and then recycled to plant nutrient solution. (4) Nutrient solution recycling. In the overall water cycle process, the plant nutrient solution was continuously self-circulated and the water quality of which was maintained at a relatively stable level with total organic carbon of 20-30 mg/L and NH4+-N < 1.0 mg/L. The 180-day continuous operation demonstrated that a 100% water closure was achieved. Based on the results of this study, an upgraded water cycle system for larger-scale and longer-term CELSS has been proposed.

Effect of nitrate recycling ratio on simultaneous biological nutrient removal in a novel anaerobic/anoxic/oxic (A2/O)-biological aerated filter (BAF) system.

A novel system integrating anaerobic/anoxic/oxic (A(2)/O) and biological aerated filter (BAF), which could solve the sludge retention time (SRT) conflicting problem between nitrifiers and polyphosphate accumulating organisms (PAOs) by shortening SRT for PAOs in A(2)/O and lengthening SRT for nitrifiers in BAF, was investigated in this study. Various nitrate recycling ratios (100%, 200%, 300% and 400%) were applied to a lab-scaled A(2)/O-BAF system to detect the simultaneous biological nitrogen and phosphorus removal performance while treating real domestic wastewater with low carbon to nitrogen (C/N) ratio. The concentrations of chemical oxygen demand (COD), NH(4)(+)-N and total phosphorus (TP) in the effluent were less than 50.0, 0.5 and 0.5mg/L, respectively, throughout the experiments. The removal efficiencies of total nitrogen (TN) were 64.9%, 77.0%, 82.0% and 87.0%, under respective nitrate recycling ratios (increasing from 100% to 400%). By contrast, nitrate recycling ratios had neglectable effect on the removal efficiencies of COD and NH(4)(+)-N.

[Effect of internal recycle ratio on nitrogen and phosphorus removal characteristics in A2/O-BAF process].

The behaviors of biological phosphorus (P) and nitrogen (N) removal in a lab-scaled anaerobic/anoxic/oxic-biological aerated filter (A2/O-BAF) combined system were investigated during the treatment of real domestic wastewater with the temperature at 15 degrees C, the C/N ratio of 4.9 and internal recycle ratio of 100%, 200%, 300% and 400%. Experimental results clearly showed that COD, N and P can be simultaneously deeply removed in this combined system. When the total HRT was 8.0 h, SRT was 15 d,sludge recycle ratio was 100% and MLSS was 4.0 mg x L(-1), the concentrations of COD, total phosphorus (TP) and ammonia nitrogen could be reached to less than 50.0, 0.5 and 1.0 mg x L(-1) in the effluent, respectively. The concentrations of total nitrogen (TN) could be reduced from 70.9, 72.1, 70.6 and 73.3 mg x L(-1) in the raw wastewater to that of 24.8, 16.5, 9.6 and 8.7 mg x L(-1) in the effluent, respectively. The removal efficiencies of TN were 65.0%, 77.1%, 86.4% and 88.1%, respectively. There was no distinct relationship between the internal recycle ratio and the removal efficiencies of COD, TP and ammonia nitrogen. However, the removal efficiencies of TN increased with the increasing of the internal recycle ratio, the rising rate was descending. Both the capacity of denitrifying and phosphorus removal in anoxic zone increased simultaneously with the increasing of the internal recycle ratio. Batch tests indicated that the population of denitrifying polyphosphate-accumulating organisms (DPAOs) was up to 40.5% of the total phosphate-accumulating organisms (PAOs).