RESUMO
BACKGROUND: The Shiga toxin-producing Escherichia coli (STEC) infects animals and induces acute intestinal inflammation. Long non-coding RNAs (lncRNAs) are known to play crucial roles in modulating inflammation response. However, it is not clear whether lncRNAs are involved in STEC-induced inflammation. METHODS AND RESULTS: To understand the association of lncRNAs with STEC infection, we used RNA-seq technology to analyze the profiles of lncRNAs in Mock-infected and STEC-infected human intestinal epithelial cells (HIECs). We detected a total of 702 lncRNAs differentially expressed by STEC infection. 583 differentially expressed lncRNAs acted as competitive microRNAs (miRNAs) binding elements in regulating the gene expression involved in TNF signaling pathway, IL-17 signaling pathway, PI3K-Akt signaling pathway, and apoptosis pathways. We analyzed 3 targeted genes, TRADD, TRAF1 and TGFB2, which were differentially regulated by mRNA-miRNA-lncRNA interaction network, potentially involved in the inflammatory and apoptotic response to STEC infection. Functional analysis of up/downstream genes associated with differentially expressed lncRNAs revealed their role in adheres junction and endocytosis. We also used the qRT-PCR technique to validate 8 randomly selected differentially expressed lncRNAs and mRNAs in STEC-infected HIECs. CONCLUSION: Our results, for the first time, revealed differentially expressed lncRNAs induced by STEC infection of HIECs. The results will help investigate the molecular mechanisms for the inflammatory responses induced by STEC.
Assuntos
MicroRNAs , RNA Longo não Codificante , Escherichia coli Shiga Toxigênica , Animais , Humanos , Escherichia coli Shiga Toxigênica/genética , Escherichia coli Shiga Toxigênica/metabolismo , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo , RNA-Seq , Fosfatidilinositol 3-Quinases/genética , MicroRNAs/genética , Inflamação , Células Epiteliais/metabolismo , Perfilação da Expressão GênicaRESUMO
Total solids (TS) content may provide a regulatory strategy for optimizing anaerobic digestion enhanced by high-temperature thermal hydrolysis, but the role of TS content is not yet clear. In this study, the effect of TS content on the high-temperature thermal hydrolysis and anaerobic digestion of sludge and its mechanism were investigated. The results showed that increasing the TS content from 2% to 8% increased the sludge solubility and methane production potential, reaching peak values of 26.6% and 336 ± 6 mL/g volatile solids (VS), respectively. With a further increase in TS content to 12%, the strong Maillard reaction increased the aromaticity and structural stability of extracellular polymer substances, decreasing sludge solubility to 18.6%. Furthermore, the decrease in sludge biodegradability and the formation of inhibitory by-products resulted in a reduction in methane production to 272 ± 4 mL/g VS. This article provides a new perspective to understand the role of TS content in the thermal hydrolysis of sludge and a novel approach to regulate the Maillard reaction.
Assuntos
Metano , Esgotos , Esgotos/química , Hidrólise , Anaerobiose , Metano/química , Metano/metabolismo , Biodegradação Ambiental , Eliminação de Resíduos Líquidos/métodos , Temperatura AltaRESUMO
Shallow lake ecosystems are highly sensitive to temperature fluctuation because of their high water surface-to-volume ratios. Shallow lakes have been increasingly identified as a hotspot of CO2 and CH4 emissions, but their response to temperature variation remains unclear. Here, we report from a 5-month outdoor mesocosm experiment where we investigated the impacts of a projected 3.5 °C future warming and monthly temperature changes on lake CO2 and CH4, as well as the key drivers affecting the lake carbon cycling. Our results show that CO2 and CH4 concentrations had a significantly positive correlation with monthly temperatures. CH4 concentration was primarily regulated by monthly temperature, while nutrients effects on CO2 concentration overrode climate warming and temporal temperature changes. These findings imply the varied roles that temperature and nutrient levels can play on CO2 and CH4 dynamics in shallow lake systems. The relationship between temperature and CO2 concentration was nonlinear, showing a threshold of approximately 9 °C, at which CO2 concentration could be strongly modified by nutrient level in the lake systems. Understanding this complex relationship between temperature with CO2 and CH4 concentrations in shallow lakes is crucial for effective lake management and efficient control of greenhouse gases (GHGs) emissions.