Reducing Greenhouse Gas Emissions from U.S. Light-Duty Transport in Line with the 2 °C Target.

Making, driving, and disposing of U.S. light-duty vehicles (LDVs) account for 3% of global greenhouse gas emissions related to energy and processing. This study calculates future emissions and global temperature rises attributable to U.S. LDVs. We examine how 2021-2050 U.S. LDV cumulative emissions can be limited to 23.1 Gt CO2equiv, helping to limit global warming to less than 2 °C. We vary four vehicle life cycle parameters (transport demand, sales share of alternative fuel vehicles, vehicle material recycling rates, and vehicle lifespans) in a dynamic fleet analysis to determine annual LDV sales, scrappage, and fleet compositions. We combine these data with vehicle technology and electricity emission scenarios to calculate annual production, use, and disposal emissions attributable to U.S. LDVs. Only 3% of the 1512 modeled pathways stay within the emission limit. Cumulative emissions are most sensitive to transport demand, and the speed of fleet electrification and electricity decarbonization. Increasing production of battery electric vehicles (BEVs) to 100% of sales by 2040 (at the latest) is necessary, and early retirement of internal combustion engine vehicles is beneficial. Rapid electricity decarbonization minimizes emissions from BEV use and increasingly energy-intensive vehicle production. Deploying high fuel economy vehicles can increase emissions from the production of BEV batteries and lightweight materials. Increased recycling has a small effect on these emissions because over the time period there are few postconsumer batteries and lightweight materials available for recycling. Without aggressive actions, U.S. LDVs will likely exceed the cumulative emissions budget by 2039 and contribute a further 0.02 °C to global warming by 2050, 2.7% of the remaining global 2 °C budget.

Mapping the Annual Flow of Steel in the United States.

A detailed understanding of material flows is needed to target increased material efficiency and circular economy. In this article, the U.S. steel flow is modeled as a series of nodes representing processes and products. An easily updatable nonlinear least squares optimization is used to reconcile the inconsistencies across 293 collated data records on flows through and between the nodes. The data come from an integrated analysis that includes top-down estimates of steel flow from trade bodies and government statistical agencies, bottom-up estimates of the steel embedded in products based on production statistics and bills of materials, and the mass of imports and exports based on international money flow. A weighting methodology is used to consistently assign confidence scores to the data, and the optimization is used to achieve mass balance and minimize the sum of the squares of the weighted residuals. The results indicate that yield improvement efforts should focus on sheet metal forming in the car industry, which accounts for nearly half of all generated fabrication scrap. The quantity of end-of-life scrap exported and land-filled is greater than the quantity of steel products imported. Increased domestic recycling of end-of-life scrap might displace around a third of these imports.

Circular RNA circ_0003028 contributes to tumorigenesis by regulating GOT2 via miR-1298-5p in non-small cell lung cancer.

Non-small cell lung cancer (NSCLC) is a common malignant tumor, with high morbidity and mortality. Circular RNA (circRNA) circ_0003028 was reported to be upregulated in NSCLC. This study is designed to explore the role and mechanism of circ_0003028 on NSCLC progression. In this work, circ_0003028, microRNA-1298-5p (miR-1298-5p), and glutamic oxaloacetic transaminase 2 (GOT2) level were detected by real-time quantitative polymerase chain reaction (RT-qPCR). The localization of circ_0003028 was analyzed by subcellular fractionation assay. Cell proliferation, colony number, cell cycle progression, apoptosis, migration, invasion, and angiogenesis were measured by Cell Counting Kit-8 (CCK-8), colony formation, flow cytometry, transwell, and tube formation assays. Protein levels of Beclin1, light chain 3 (LC3)-II/LC3-I, GOT2, proliferating cell nuclear antigen (PCNA) were examined by western blot assay. The binding relationship between miR-1298-5p and circ_0003028 or GOT2 was predicted by circular RNA Interactome or starbase and then verified by dual-luciferase reporter, RNA Immunoprecipitation (RIP), and RNA pull-down assays. The biological role of circ_0003028 on NSCLC tumor growth was examined by the xenograft tumor model in vivo. We reported that circ_0003028 and GOT2 were upregulated, and miR-1298-5p was decreased in NSCLC tissues and cells. Moreover, circ_0003028 knockdown curbed cell proliferative ability, migration, invasion, angiogenesis, and facilitate apoptosis and autophagy in NSCLC cells in vitro. Mechanical analysis discovered that circ_0003028 regulated GOT2 expression by sponging miR-1298-5p. Circ_0003028 silencing hindered the cell growth of NSCLC in vivo. Taken together, circ_0003028 knockdown could suppress NSCLC progression partly by regulating the miR-1298-5p/GOT2 axis, providing an underlying therapeutic target for NSCLC.