RESUMO
The objective was to characterize the transmission of Toxoplasma gondii in goats experimentally infected vaginally with semen contaminated with the CPG strain (genotype III). Ten female goats were randomly allocated into 2 groups (G1 and G2), each with 5 animals, and inseminated during estrus. Goats in G1 were inseminated with semen containing 1 × 10(5) tachyzoites, whereas those in G2 (control) were inseminated with semen free from tachyzoites (insemination = day 0). In G1, seroconversion (indirect immunofluorescence reaction) and DNA (polymerase chain reaction) in the blood was present in 4/5 and 3/5, respectively, from the 7th day. In G2, all goats were negative in all tests. Embryonic reabsorption occurred in 4 of 5 goats from G1 between days 21 and 49. In conclusion, artificial vaginal insemination with semen containing tachyzoites of T. gondii -infected goats and is a potential transmission route of this parasite through semen.
Assuntos
Doenças das Cabras/transmissão , Sêmen/parasitologia , Toxoplasmose Animal/transmissão , Animais , Anticorpos Antiprotozoários/sangue , Brasil/epidemiologia , DNA de Protozoário/sangue , Feminino , Doenças das Cabras/epidemiologia , Doenças das Cabras/parasitologia , Cabras , Inseminação Artificial/veterinária , Masculino , Camundongos , Gravidez , Resultado da Gravidez/veterinária , Testes de Gravidez/veterinária , Toxoplasma/classificação , Toxoplasma/genética , Toxoplasma/imunologia , Toxoplasmose Animal/epidemiologia , Ultrassonografia Pré-Natal/veterináriaRESUMO
We report the finding of Amblyomma aureolatum (Acari: Ixodidae) parasitizing margay (Leopardus wiedii) in the locality of Gravataí (29° 47' 12.9" S and 50° 53' 44.1" W; 241 m alt.), State of Rio Grande do Sul, Southern Brazil. This is the first report of this species of Ixodidae on margay in Southern Brazil. This finding indicates an increased number of hosts for this tick species.