Caracterización molecular de mecanismos de resistencia a cloranfenicol en cepas de shigella flexneri aisladas en niños chilenos con diarrea aguda / Molecular characterization of resistance mechanisms to chloramphenicol in shigella flexneri strains isolated from chilean children with acute diarrhea
Rev. méd. Chile
; 130(3): 275-280, mar. 2002. ilus, tab
Article
em Es
| LILACS
| ID: lil-314853
Biblioteca responsável:
CL1.1
ABSTRACT
Background:
Chloramphenicol is one of the therapeutic options for shigellosis, but resistance to this antimicrobial is increasing.Aim:
To characterize molecular mechanisms conferring resistance to chloramphenicol (CmR) in Shigella flexneri strains isolated from Chilean children with acute diarrhea. Material andmethods:
Thirty one Shigella filexneri strains, including 22 with the CmR phenotype were analyzed. Strains were tested for antimicrobial susceptibility by plate dilution and for the presence of an internal fragment of the cat gene encoding for chloramphenicol o-acetyl-transferase, by polymerase chain reaction and Southern blot analysis.Results:
All CmR strains had a minimal inhibitory concentration over 64 µg/ml and amplified the internal fragment of the cat gene. Southern blot analyses indicated that this gene was located in the bacterial chromosome.Conclusions:
Resistance to chloramphenicol in this group of Shigella flexneri strains was mediated by a chromosomally located cat gene
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Shigella flexneri
/
Resistência ao Cloranfenicol
/
Diarreia
Limite:
Child
/
Humans
País/Região como assunto:
America do sul
/
Chile
Idioma:
Es
Revista:
Rev. méd. Chile
Assunto da revista:
MEDICINA
Ano de publicação:
2002
Tipo de documento:
Article
/
Project document
País de afiliação:
Chile