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An automated system for screening retroviral expression constructs in microplate format.
Stump, Mark D; Di Sera, Leonardo; Rebentisch, Matthew; Endo, Mark; Pierce, Michael; Kamb, Alexander.
Afiliação
  • Stump MD; Deltagen Proteomics, Inc., Arcaris, Inc., Salt Lake City, UT 84108, USA. stump@arcaris.com
J Biomol Screen ; 7(3): 275-80, 2002 Jun.
Article em En | MEDLINE | ID: mdl-12097190
Retroviruses are useful for genetics studies to deliver genes that express proteins, peptides, and RNAs. Several steps, including DNA preparation, transfection, packaging, transduction, and assay, are required to execute screens using retroviral constructs. Unlike screens with purified components, whole-cell assays using retroviral constructs need a large number of steps with microplate manipulations. The nature of these steps, especially the involvement of cultured mammalian cells, limits the throughput of such screens. To improve the efficiency of genetic experiments with retroviral expression vectors, an automated system for retroviral screening in microplates was devised and tested. The system, called Somata, provides high throughputs and robust, reproducible performance.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Retroviridae / Vetores Genéticos Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Humans Idioma: En Revista: J Biomol Screen Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2002 Tipo de documento: Article País de afiliação: Estados Unidos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Retroviridae / Vetores Genéticos Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Humans Idioma: En Revista: J Biomol Screen Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2002 Tipo de documento: Article País de afiliação: Estados Unidos