Objective method of comparing DNA microarray image analysis systems.
Biotechniques
; 36(6): 960-7, 2004 Jun.
Article
em En
| MEDLINE
| ID: mdl-15211746
ABSTRACT
Many image analysis systems are available for processing the images produced by laser scanning of DNA microarrays. The image processing system takes pixel-level intensity data and converts it to a set of gene-level expression or copy number summaries that will be used in further analyses. Image analysis systems currently in use differ with regard to the specific algorithms they implement, ease of use, and cost. Thus, it would be desirable to have an objective means of comparing systems. Here we describe a systematic method of comparing image processing results produced by different image analysis systems using a series of replicate microarray experiments. We demonstrate the method with a comparison of cDNA microarray data generated by the UCSF Spot and the GenePix image processing systems.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Algoritmos
/
Reconhecimento Automatizado de Padrão
/
Interpretação de Imagem Assistida por Computador
/
Microscopia Confocal
/
Análise de Falha de Equipamento
/
Análise de Sequência com Séries de Oligonucleotídeos
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Idioma:
En
Revista:
Biotechniques
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Estados Unidos