The bacteriophage lambda DNA replication protein P inhibits the oriC DNA- and ATP-binding functions of the DNA replication initiator protein DnaA of Escherichia coli.
J Biochem Mol Biol
; 38(1): 97-103, 2005 Jan 31.
Article
em En
| MEDLINE
| ID: mdl-15715953
ABSTRACT
Under the condition of expression of lambda P protein at lethal level, the oriC DNA-binding activity is significantly affected in wild-type E. coli but not in the rpl mutant. In purified system, the lambda P protein inhibits the binding of both oriC DNA and ATP to the wild-type DnaA protein but not to the rpl DnaA protein. We conclude that the lambda P protein inhibits the binding of oriC DNA and ATP to the wild-type DnaA protein, which causes the inhibition of host DNA synthesis initiation that ultimately leads to bacterial death. A possible beneficial effect of this interaction of lambda P protein with E. coli DNA initiator protein DnaA for phage DNA replication has been proposed.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Proteínas Virais
/
DNA Bacteriano
/
Trifosfato de Adenosina
/
Bacteriófago lambda
/
Origem de Replicação
/
Proteínas de Ligação a DNA
/
Replicação do DNA
Idioma:
En
Revista:
J Biochem Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Índia