The H2O2-regulated Ep5C gene encodes a peroxidase required for bacterial speck susceptibility in tomato.
Plant J
; 42(2): 283-93, 2005 Apr.
Article
em En
| MEDLINE
| ID: mdl-15807789
ABSTRACT
Bacterial speck caused by the pathogen Pseudomonas syringae pv. tomato (P. s. tomato) is a devastating disease of tomato plants. Here we show that inhibition of Ep5C gene expression, which encodes a secreted cationic peroxidase, is sufficient to confer resistance against P. s. tomato. The inhibition of Ep5C protein accumulation in antisense tomato plants established resistance that was not accompanied by the pre-activation of known defense pathways. Therefore, Ep5C inhibition represents a novel form of disease resistance based on a loss-of-gene function in the plant required for successful infection by a compatible bacterial pathogen. Ep5C expression is rapidly induced by H2O2, a reactive oxygen intermediate normally generated during the course of a plant-pathogen interaction. This was corroborated by monitoring the expression of an Ep5C-GUS gene in transgenic Arabidopsis plants. Collectively, these results identify a signaling pathway that uses early signals generated during the oxidative burst, such as H2O2, for the selective activation of host factors required for mounting a compatible interaction. Thus, Ep5C provides a new resource for developing bacterial speck disease-resistant varieties.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peroxidases
/
Solanum lycopersicum
/
Peróxido de Hidrogênio
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Plant J
Assunto da revista:
BIOLOGIA MOLECULAR
/
BOTANICA
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Espanha