Multigenotype HCV-NS3 recombinant vaccinia viruses as a model for evaluation of cross-genotype immunity induced by HCV vaccines in the mouse.
Vaccine
; 24(24): 5140-8, 2006 Jun 12.
Article
em En
| MEDLINE
| ID: mdl-16713033
ABSTRACT
Surrogate infections with HCV-recombinant vaccinia viruses (HCV-rVV) are a standard method to test the efficacy of hepatitis C virus (HCV) vaccine candidates in the mouse model. We established a panel of 16 HCV-rVV expressing the nonstructural protein 3 (NS3) of HCV genotypes 1a, 1b, 2, 3 and 4. Mice immunized with recombinant NS3 protein derived from HCV genotype 1b were challenged with the rVV. rVV-titers decreased up to 54-fold after subtype 1b challenge and up to 8.5-fold after subtype 1a challenge. No change was detected for genotype 2, 3, or 4. Our model is a convenient and reliable tool to analyze the induction of cross-genotype immunity by experimental vaccination of mice.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vaccinia virus
/
Vacinas contra Hepatite Viral
/
Vacinas Sintéticas
/
Proteínas não Estruturais Virais
/
Hepacivirus
Limite:
Animals
Idioma:
En
Revista:
Vaccine
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Alemanha