Postsynaptic membrane addition depends on the Discs-Large-interacting t-SNARE Gtaxin.
J Neurosci
; 27(5): 1033-44, 2007 Jan 31.
Article
em En
| MEDLINE
| ID: mdl-17267557
ABSTRACT
Targeted membrane addition is a hallmark of many cellular functions. In the nervous system, modification of synaptic membrane size has a major impact on synaptic function. However, because of the complex shape of neurons and the need to target membrane addition to very small and polarized synaptic compartments, this process is poorly understood. Here, we show that Gtaxin (GTX), a Drosophila t-SNARE (target-soluble N-ethylmaleimide-sensitive factor attachment protein receptor), is required for expansion of postsynaptic membranes during new synapse formation. Mutations in gtx lead to drastic reductions in postsynaptic membrane surface, whereas gtx upregulation results in the formation of complex membrane structures at ectopic sites. Postsynaptic GTX activity depends on its direct interaction with Discs-Large (DLG), a multidomain scaffolding protein of the PSD-95 (postsynaptic density protein-95) family with key roles in cell polarity and formation of cellular junctions as well as synaptic protein anchoring and trafficking. We show that DLG selectively determines the postsynaptic distribution of GTX to type I, but not to type II or type III boutons on the same cell, thereby defining sites of membrane addition to this unique set of glutamatergic synapses. We provide a mechanistic explanation for selective targeted membrane expansion at specific synaptic junctions.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Membranas Sinápticas
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Terminações Pré-Sinápticas
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Proteínas de Drosophila
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Proteínas Supressoras de Tumor
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Proteínas SNARE
Limite:
Animals
Idioma:
En
Revista:
J Neurosci
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos