Measurement of aflatoxin and aflatoxin metabolites in urine by liquid chromatography-tandem mass spectrometry.
J Anal Toxicol
; 31(3): 150-6, 2007 Apr.
Article
em En
| MEDLINE
| ID: mdl-17579962
ABSTRACT
Automated immunoaffinity solid-phase extraction followed by liquid chromatography-tandem mass spectrometry and chemical analogue internal standardization is employed to detect and quantify the aflatoxins AFB(1), AFB(2), AFG(1), AFG(2), and the metabolites AFM(1) and AFP(1) in urine. The dynamic range of the method is nearly three orders of magnitude with limits of detection in the low femtogram on column range. The method was validated over a 12-day period by eight analysts. This method is suitable for agricultural, forensic, and public health laboratories during an accidental outbreak or a chemical terrorism event where a rapid and accurate diagnosis of aflatoxicosis is needed.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida de Alta Pressão
/
Aflatoxinas
/
Espectrometria de Massas em Tandem
Limite:
Animals
Idioma:
En
Revista:
J Anal Toxicol
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos