Cellular absorption of anthraquinones emodin and chrysophanol in human intestinal Caco-2 cells.
Biosci Biotechnol Biochem
; 71(7): 1636-43, 2007 Jul.
Article
em En
| MEDLINE
| ID: mdl-17617731
ABSTRACT
The intestinal absorption characteristics of anthraquinones emodin and chrysophanol were observed by measuring the intracellular accumulation across Caco-2 cells by the reverse-phase high performance liquid chromatography. The intracellular accumulation of chrysophanol was much greater than that of emodin, the maximum absorption of emodin and chrysophanol being 414.02+/-15.28 and 105.56+/-11.57 nmol/l x mg x protein, respectively. The absorption of each anthraquinone was significantly lower at 4 degrees C than that of 37 degrees C. The effects of the transport inhibitors, verapamil, cyclosporine and phloridzin, on the intracellular accumulation were also examined. Verapamil and cyclosporine increased the absorption of emodin and chrysophanol, while phloridzin inhibited their absorption, all in a dose-dependent manner. These results suggest that the absorption characteristics of emodin and chrysophanol were closely related to their special structure with the hydroxy groups. It is also likely that a specific transport system mediated the intracellular accumulation of emodin and chrysophanol across the Caco-2 cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Antraquinonas
/
Emodina
/
Inibidores Enzimáticos
/
Mucosa Intestinal
/
Mutagênicos
Limite:
Humans
Idioma:
En
Revista:
Biosci Biotechnol Biochem
Assunto da revista:
BIOQUIMICA
/
BIOTECNOLOGIA
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
China