Asterless is a centriolar protein required for centrosome function and embryo development in Drosophila.
Curr Biol
; 17(20): 1735-45, 2007 Oct 23.
Article
em En
| MEDLINE
| ID: mdl-17935995
ABSTRACT
BACKGROUND:
Centrosomes, the major organizers of the microtubule network in most animal cells, are composed of centrioles embedded in a web of pericentriolar material (PCM). Recruitment and stabilization of PCM on the centrosome is a centriole-dependent function. Compared to the considerable number of PCM proteins known, the molecular characterization of centrioles is still very limited. Only a few centriolar proteins have been identified so far in Drosophila, most related to centriole duplication.RESULTS:
We have cloned asterless (asl) and found that it encodes a 120 kD highly coiled-coil protein that is a constitutive pancentriolar and basal body component. Loss of asl function impedes the stabilization/maintenance of PCM at the centrosome. In embryos deficient for Asl, development is arrested right after fertilization. Asl shares significant homology with Cep 152, a protein described as a component of the human centrosome for which no functional data is yet available.CONCLUSIONS:
The cloning of asl offers new insight into the molecular composition of Drosophila centrioles and a possible model for the role of its human homolog. In addition, the phenotype of asl-deficient flies reveals that a functional centrosome is required for Drosophila embryo development.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Centríolos
/
Centrossomo
/
Proteínas de Drosophila
/
Drosophila melanogaster
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Curr Biol
Assunto da revista:
BIOLOGIA
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Alemanha