Organelle tethering by a homotypic PDZ interaction underlies formation of the Golgi membrane network.
J Cell Biol
; 186(1): 41-55, 2009 Jul 13.
Article
em En
| MEDLINE
| ID: mdl-19581411
ABSTRACT
Formation of the ribbon-like membrane network of the Golgi apparatus depends on GM130 and GRASP65, but the mechanism is unknown. We developed an in vivo organelle tethering assaying in which GRASP65 was targeted to the mitochondrial outer membrane either directly or via binding to GM130. Mitochondria bearing GRASP65 became tethered to one another, and this depended on a GRASP65 PDZ domain that was also required for GRASP65 self-interaction. Point mutation within the predicted binding groove of the GRASP65 PDZ domain blocked both tethering and, in a gene replacement assay, Golgi ribbon formation. Tethering also required proximate membrane anchoring of the PDZ domain, suggesting a mechanism that orientates the PDZ binding groove to favor interactions in trans. Thus, a homotypic PDZ interaction mediates organelle tethering in living cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Domínios PDZ
/
Complexo de Golgi
/
Membranas Intracelulares
/
Proteínas de Membrana
Limite:
Humans
Idioma:
En
Revista:
J Cell Biol
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Estados Unidos