Optical trapping and laser ablation of microtubules in fission yeast.

ABSTRACT

Manipulation has been used as a powerful investigation technique since the early history of biology. Every technical advance resulted in more refined instruments that led to the discovery of new phenomena and to the solution of old problems. The invention of laser in 1960 gave birth to what is now called optical manipulation the use of light to interact with matter. Since then, the tremendous progress of laser technology made optical manipulation not only an affordable, reliable alternative to traditional manipulation techniques but disclosed also new, intriguing applications that were previously impossible, such as contact-free manipulation. Currently, optical manipulation is used in many fields, yet has the potential of becoming an everyday technique in a broader variety of contexts. Here, we focus on two main optical manipulation techniques optical trapping and laser ablation. We illustrate with selected applications in fission yeast how in vivo optical manipulation can be used to study organelle positioning and the force balance in the microtubule cytoskeleton.