Pseudomonas aeruginosa accentuates epithelial-to-mesenchymal transition in the airway.
Eur Respir J
; 37(5): 1237-47, 2011 May.
Article
em En
| MEDLINE
| ID: mdl-20847079
ABSTRACT
Epithelial-to-mesenchymal transition (EMT) has been implicated in the dysregulated epithelial wound repair that contributes to obliterative bronchiolitis (OB) after lung transplantation. Acquisition of Pseudomonas aeruginosa in the transplanted airway has been shown to be a risk factor for the development of OB. We investigated the potential of P. aeruginosa to drive EMT in primary bronchial epithelial cells (PBECs) isolated from lung transplant recipients. Changes in the expression of epithelial and mesenchymal markers was assessed in cells challenged with clinical isolates of P. aeruginosa or co-cultured with P. aeruginosa-activated monocytic cells (THP-1) in the presence or absence of transforming growth factor (TGF)-ß1. P. aeruginosa did not drive or accentuate TGF-ß1-driven EMT directly. Co-culturing P. aeruginosa-activated THP-1 cells with PBECs did not drive EMT. However, co-culturing P. aeruginosa-activated THP-1 cells with PBECs significantly accentuated TGF-ß1-driven EMT. P. aeruginosa, via the activation of monocytic cells, can accentuate TGF-ß1-driven EMT. These in vitro observations may help explain the in vivo clinical observation of a link between acquisition of P. aeruginosa and an increased risk of developing OB.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pseudomonas aeruginosa
/
Transição Epitelial-Mesenquimal
Tipo de estudo:
Risk_factors_studies
Limite:
Humans
Idioma:
En
Revista:
Eur Respir J
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Reino Unido