Structure of the dengue virus glycoprotein non-structural protein 1 by electron microscopy and single-particle analysis.
J Gen Virol
; 93(Pt 4): 771-779, 2012 Apr.
Article
em En
| MEDLINE
| ID: mdl-22238236
ABSTRACT
The flavivirus non-structural protein 1 (NS1) is a glycoprotein that is secreted as a soluble hexameric complex during the course of natural infection. Growing evidence indicates that this secreted form of NS1 (sNS1) plays a significant role in immune evasion and modulation during infection. Attempts to determine the crystal structure of NS1 have been unsuccessful to date and relatively little is known about the macromolecular organization of the sNS1 hexamer. Here, we have applied single-particle analysis to images of baculovirus-derived recombinant dengue 2 virus NS1 obtained by electron microscopy to determine its 3D structure to a resolution of 23 Å. This structure reveals a barrel-like organization of the three dimeric units that comprise the hexamer and provides further insights into the overall organization of oligomeric sNS1.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas não Estruturais Virais
/
Vírus da Dengue
Limite:
Animals
Idioma:
En
Revista:
J Gen Virol
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Austrália