Global radiation damage: temperature dependence, time dependence and how to outrun it.
J Synchrotron Radiat
; 20(Pt 1): 7-13, 2013 Jan.
Article
em En
| MEDLINE
| ID: mdl-23254651
ABSTRACT
A series of studies that provide a consistent and illuminating picture of global radiation damage to protein crystals, especially at temperatures above â¼200â
K, are described. The radiation sensitivity shows a transition near 200â
K, above which it appears to be limited by solvent-coupled diffusive processes. Consistent with this interpretation, a component of global damage proceeds on timescales of several minutes at 180â
K, decreasing to seconds near room temperature. As a result, data collection times of order 1â
s allow up to half of global damage to be outrun at 260â
K. Much larger damage reductions near room temperature should be feasible using larger dose rates delivered using microfocused beams, enabling a significant expansion of structural studies of proteins under more nearly native conditions.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas
Idioma:
En
Revista:
J Synchrotron Radiat
Assunto da revista:
RADIOLOGIA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Estados Unidos