CD4+ T-cell activation impairs serogroup C Neisseria meningitis vaccine response in HIV-infected children.
AIDS
; 27(17): 2697-705, 2013 Nov 13.
Article
em En
| MEDLINE
| ID: mdl-24149087
ABSTRACT
OBJECTIVE:
To investigate the influence of CD4 T-cell activation and regulatory populations in HIV-infected children antibody response to vaccination with a conjugate C polysaccharide vaccine.DESIGN:
CD4 T-cell activation was evaluated by expression of CD38, HLA-DR and CCR5 molecules. Regulatory CD4 T cells (TReg) were characterized as FoxP3CD127CD25 and inducer T cells (TInd) as CD4FoxP3CD25CD39.METHODS:
All patients (nâ=â36) were HIV-vertically infected, aged 2-17 years-old and were vaccinated with one vaccine injection. Blood samples were obtained before and after immunization to determine bactericidal antibody titers (SBA), CD4 T-cell activation and frequency of TReg and TInd subsets (multiparametric flow cytometry).RESULTS:
Children not-responding (nâ=â18) to MenC vaccine expressed higher frequency of activated CD4 T cells (HLA-DRCD38CCR5) than responders (nâ=â18), both before and after vaccination (Pâ<â0.05). A significant higher frequency of TReg was detected in responders compared with nonresponders (Pâ=â0.0001). We also detected an inverse correlation between CD4DRCD38CCR5 (Pâ=â0.01) or CD4DRCD38 (Pâ=â0.02) T cells and TReg cell frequency after vaccination. CD4 T-cell activation negatively correlated (Pâ=â0.006) with postvaccination SBA titers but a positive correlation (Pâ=â0.0001) was detected between TReg cells and SBA. TReg and TInd subsets were inversely correlated (Pâ=â0.04).CONCLUSION:
Our findings suggest that higher CD4 T-cell activation leads to poor vaccine response in children living with HIV, which may be associated with a TReg/TInd disequilibrium.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ativação Linfocitária
/
Linfócitos T CD4-Positivos
/
Infecções por HIV
/
Vacinas Meningocócicas
Tipo de estudo:
Etiology_studies
/
Incidence_studies
/
Observational_studies
/
Risk_factors_studies
Limite:
Adolescent
/
Child
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Child, preschool
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
AIDS
Assunto da revista:
SINDROME DA IMUNODEFICIENCIA ADQUIRIDA (AIDS)
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Brasil