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Comparing the genetic diversity of ORF30 of Australian isolates of 3 equid alphaherpesviruses.
Cuxson, Jennifer L; Hartley, Carol A; Ficorilli, Nino P; Symes, Sally J; Devlin, Joanne M; Gilkerson, James R.
Afiliação
  • Cuxson JL; Centre for Equine Infectious Disease, Faculty of Veterinary Science, The University of Melbourne, Victoria 3010, Australia.
  • Hartley CA; Centre for Equine Infectious Disease, Faculty of Veterinary Science, The University of Melbourne, Victoria 3010, Australia.
  • Ficorilli NP; Centre for Equine Infectious Disease, Faculty of Veterinary Science, The University of Melbourne, Victoria 3010, Australia.
  • Symes SJ; Centre for Equine Infectious Disease, Faculty of Veterinary Science, The University of Melbourne, Victoria 3010, Australia.
  • Devlin JM; Centre for Equine Infectious Disease, Faculty of Veterinary Science, The University of Melbourne, Victoria 3010, Australia.
  • Gilkerson JR; Centre for Equine Infectious Disease, Faculty of Veterinary Science, The University of Melbourne, Victoria 3010, Australia. Electronic address: jrgilk@unimelb.edu.au.
Vet Microbiol ; 169(1-2): 50-7, 2014 Feb 21.
Article em En | MEDLINE | ID: mdl-24418044
A single nucleotide polymorphism (SNP) has been previously associated with EHV-1 neurological disease in several countries around the world. This disease is very uncommon in Australia and little information is available about the presence of this SNP in Australian EHV-1 isolates. The ORF30 sequence of 66 Australian EHV-1 isolates was determined and the genotype was compared to the disease manifestation of the case from which the virus was isolated. Of the 66 isolates, 61 were from cases of abortion and 5 were cases associated with equine herpesvirus myeloencephalopathy (EHM). There was no association between pathotype and genotype in these isolates. In total, 64 of the 66 isolates encoded N752, including 4 isolates from EHM cases. The ORF30 sequence was also determined for 14 EHV-4 isolates, including 2 isolates from confirmed EHV-4 abortion cases. All 14 EHV-4 isolates had aspartic acid at the position equivalent to EHV-1 AA752. Aspartic acid was also confirmed in this position for the single isolate of AHV-3 sequenced in this study. The nucleotide sequence of ORF68 was also determined and showed considerable genetic heterogeneity in the EHV-1 isolates, however, this ORF was highly conserved among the 14 EHV-4 isolates sequenced, with only one SNP identified among 7 isolates. These results confirm that the EHV1 ORF30 N752 is unique and that the D752 sequence is most likely to be the true parent strain of this virus. We suggest that the abortigenic form of EHV-1 should be considered to be the more recently emerged mutant.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Variação Genética / Fases de Leitura Aberta / Varicellovirus / Herpesvirus Equídeo 1 / Infecções por Herpesviridae / Herpesvirus Equídeo 4 / Doenças dos Cavalos Limite: Animals / Pregnancy País/Região como assunto: Oceania Idioma: En Revista: Vet Microbiol Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Austrália

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Variação Genética / Fases de Leitura Aberta / Varicellovirus / Herpesvirus Equídeo 1 / Infecções por Herpesviridae / Herpesvirus Equídeo 4 / Doenças dos Cavalos Limite: Animals / Pregnancy País/Região como assunto: Oceania Idioma: En Revista: Vet Microbiol Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Austrália