Improvement of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for identification of clinically important Candida species.
Clin Lab
; 60(1): 37-46, 2014.
Article
em En
| MEDLINE
| ID: mdl-24600973
ABSTRACT
BACKGROUND:
Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) is emerging as a rapid and accurate tool for identifying pathogens. MALDI-TOF identification of Candida at the species level remains unsatisfactory because of an incomplete commercial database.METHODS:
Using Bruker Daltonics MALDI Biotyper software, an in-house spectral database library was created with m/z ratios of 2,000 to 20,000 Da from 60 successfully identified clinical isolates of Candida spp. (6 species). It was incorporated into the original database to produce the augmented Bruker-Tianjin database. The augmented database was tested with 347 clinical isolates of 6 species comparing with Bruker database in parallel.RESULTS:
The identification rates at the species level were 91.6% (318/347) using the Bruker database and 99.1% (344/347) for the Bruker-Tianjin database.CONCLUSIONS:
Database expansion and refinement can easily and effectively improve MALDI-TOF MS performance for enhanced clinical pathogen identification.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Candida
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Clin Lab
Assunto da revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
China