In cellulo structure determination of a novel cypovirus polyhedrin.
Acta Crystallogr D Biol Crystallogr
; 70(Pt 5): 1435-41, 2014 May.
Article
em En
| MEDLINE
| ID: mdl-24816111
ABSTRACT
This work demonstrates that with the use of a microfocus synchrotron beam the structure of a novel viral polyhedrin could be successfully determined from microcrystals within cells, removing the preparatory step of sample isolation and maintaining a favourable biological environment. The data obtained are of high quality, comparable to that obtained from isolated crystals, and enabled a facile structure determination. A small but significant difference is observed between the unit-cell parameters and the mosaic spread of in cellulo and isolated crystals, suggesting that even these robust crystals are adversely affected by removal from the cell.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Reoviridae
/
Proteínas Virais
/
Cristalografia por Raios X
Idioma:
En
Revista:
Acta Crystallogr D Biol Crystallogr
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Reino Unido