Bacteriological and virulence study of a Mycobacterium chimaera isolate from a patient in China.
Antonie Van Leeuwenhoek
; 107(4): 901-9, 2015 Apr.
Article
em En
| MEDLINE
| ID: mdl-25763937
ABSTRACT
A clinical isolate from a patient was identified as Mycobacterium chimaera, a recently identified species of nontuberculous Mycobacteria. The biochemical and molecular identity, drug sensitivity and virulence of this isolated strain were investigated. 16S rRNA, the 16S-23S ITS, hsp65 and rpoB were amplified, and their sequence similarities with other mycobacteria were analyzed. The minimum inhibitory concentrations of 22 anti-microbial agents against this isolate were established, and the virulence of the isolate was evaluated by intravenous injection into C57BL/6 mice using Mycobacterium tuberculosis H37Rv as a control strain. Growth and morphological characteristics and mycolic acid profile analysis revealed that this isolated strain was a member of the Mycobacterium avium complex. BLAST analysis of the amplified sequences showed that the isolated strain was closely related to M. chimaera. Susceptibility testing showed that the isolate was sensitive to rifabutin, rifapentine, clarithromycin, azithromycin, imipenem and cefoxitin. Bacterial load determination and tissue histopathology of the infected mice indicated that the isolate was highly virulent. The first case of M. chimaera infection in China was evaluated. The information derived from this case may offer valuable guidance for clinical diagnosis and treatment.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Mycobacterium
/
Infecções por Mycobacterium não Tuberculosas
Tipo de estudo:
Guideline
/
Prognostic_studies
Limite:
Adult
/
Animals
/
Humans
/
Male
País/Região como assunto:
Asia
Idioma:
En
Revista:
Antonie Van Leeuwenhoek
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
China