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Diagnostic performances of antigen detection compared to conventional and nucleic acid detection of Entamoeba histolytica in a non-endemic setting.
Calderaro, Adriana; Piergianni, Maddalena; Piccolo, Giovanna; Rossi, Sabina; Montecchini, Sara; Buttrini, Mirko; Arcangeletti, Maria Cristina; Medici, Maria Cristina; Chezzi, Carlo; De Conto, Flora.
Afiliação
  • Calderaro A; Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
  • Piergianni M; Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
  • Piccolo G; Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
  • Rossi S; Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
  • Montecchini S; Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
  • Buttrini M; Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
  • Arcangeletti MC; Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
  • Medici MC; Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
  • Chezzi C; Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
  • De Conto F; Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
New Microbiol ; 39(2): 153-5, 2016 Apr.
Article em En | MEDLINE | ID: mdl-27196557
ABSTRACT
This study evaluated the immunochromatographic (IC) assay "TECHLAB(®) E. HISTOLYTICA QUIK CHEK™" analysing 36 faecal samples and 7 cultured strains. This assay was compared to the methods performed in our laboratory for the diagnosis of amoebiasis. The IC assay revealed a detection limit of 103 trophozoites/g faeces and no cross-reactivity with other parasites and failed to detect E. histolytica antigen in frozen faeces. In our laboratory located in a non-endemic setting this assay could not replace the methods currently used for the diagnosis of amoebiasis.
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA de Protozoário / Entamoeba histolytica / Entamebíase / Antígenos de Protozoários Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: New Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Itália
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA de Protozoário / Entamoeba histolytica / Entamebíase / Antígenos de Protozoários Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: New Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Itália