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Association of CD36 expression and polymorphism with serum biochemical indices in Cherry Valley duck.
Wang, D D; Li, W G; Zhang, Y Y.
Afiliação
  • Wang DD; Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University, South Campus, Huaxi, Guiyang, Guizhou, China.
  • Li WG; Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University, South Campus, Huaxi, Guiyang, Guizhou, China.
  • Zhang YY; Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University, South Campus, Huaxi, Guiyang, Guizhou, China.
Genet Mol Res ; 15(2)2016 May 23.
Article em En | MEDLINE | ID: mdl-27323079
ABSTRACT
Cluster of differentiation 36 (CD36) plays a crucial role in lipid sensing, innate immunity, atherogenesis, and glycolipid metabolism. This aims of this study were to delineate the CD36 mRNA expression profile in 16 duck tissues using relative quantitative real-time PCR and to screen single nucleotide polymorphisms (SNPs) in the duck CD36 gene by PCR-single strand conformation polymorphism and DNA direct sequencing. In addition, this study investigated CD36 gene expression, genetic variation, and their effect on serum biochemical indices in duck. The results showed that CD36 mRNA was expressed in all tissues, and was highly specific to the pituitary and large intestine, and to subcutaneous and abdominal fat. Furthermore, three genotypes of the SNP g.476593 T > C in exon 9 of the duck CD36 gene were identified MM, MN, and NN. The dominant genotype and allele were MM and M, with frequencies of 0.453 and 0.643, respectively. The genotype distributions deviated from Hardy-Weinberg equilibrium (P < 0.05) and achieved moderate levels of polymorphism in ducks. Correlation results showed that CD36 mRNA was significantly negatively correlated with triglycerides (P < 0.05), and significantly positively correlated with total protein, globulin, low-density lipoprotein cholesterol, and total cholesterol (P < 0.01). All serum biochemical indices measured, with the exception of triglycerides, in birds with the NN genotype were significantly higher than those in birds with the MM genotype. These findings demonstrated that CD36 might be an important genetic marker for the selection of lipid metabolism and meat quality traits in ducks.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antígenos CD36 / Patos / Estudos de Associação Genética / Carne Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antígenos CD36 / Patos / Estudos de Associação Genética / Carne Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: China