Sprouty 2, an Early Response Gene Regulator of FosB and Mesenchymal Stem Cell Proliferation During Mechanical Loading and Osteogenic Differentiation.
J Cell Biochem
; 118(9): 2606-2614, 2017 09.
Article
em En
| MEDLINE
| ID: mdl-28387432
ABSTRACT
Sprouty 2 (Spry2), an inhibitor of MAP kinase signaling was previously shown by our group to be induced during mechanical loading of mesenchymal stem cells (MSCs). Here, we studied the implication of Spry2 activation during mechanical loading and chemically induced MSC differentiation. Spry 2 expression showed an immediate early response during mechanical loading and chemical induction of osteogenic differentiation and followed the same pattern as osteogenic associated gene FosB and was necessary for the induction of FosB, as Spry 2 knock down also abrogated the upregulation of FosB expression. Spry 2 knock down was, also associated with an early response of the osteogenic genes Runx-2 and ALP. Neither the knock-down of Spry 2 nor the subsequent reduction in FosB had any effect on mid-late osteogenesis or mineralization but was associated with a significant increase in proliferation of MSC. These effects were possibly governed by negative regulation of MEK/Erk signaling as Spry 2 knock down resulted in an increase in phosphorylation of Erk1/2. In summary, our results shows the involvement of Spry2 in regulation of FosB and Runx2 genes, MAPK signaling and proliferation of MSC. Taken together these results suggest a possible role for Spry2 in regulation of MSC functions in response to mechanical loading and osteogenic differentiation. J. Cell. Biochem. 118 2606-2614, 2017. © 2017 Wiley Periodicals, Inc.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteogênese
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Estresse Mecânico
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Diferenciação Celular
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Proteínas Proto-Oncogênicas c-fos
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Sistema de Sinalização das MAP Quinases
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Peptídeos e Proteínas de Sinalização Intracelular
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Proliferação de Células
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Células-Tronco Mesenquimais
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Proteínas de Membrana
Limite:
Humans
Idioma:
En
Revista:
J Cell Biochem
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Reino Unido