Comparative genomics and transcriptome analysis of Lactobacillus rhamnosus ATCC 11443 and the mutant strain SCT-10-10-60 with enhanced L-lactic acid production capacity.
Mol Genet Genomics
; 293(1): 265-276, 2018 Feb.
Article
em En
| MEDLINE
| ID: mdl-29159508
ABSTRACT
Mechanisms for high L-lactic acid production remain unclear in many bacteria. Lactobacillus rhamnosus SCT-10-10-60 was previously obtained from L. rhamnosus ATCC 11443 via mutagenesis and showed improved L-lactic acid production. In this study, the genomes of strains SCT-10-10-60 and ATCC 11443 were sequenced. Both genomes are a circular chromosome, 2.99 Mb in length with a GC content of approximately 46.8%. Eight split genes were identified in strain SCT-10-10-60, including two LytR family transcriptional regulators, two Rex redox-sensing transcriptional repressors, and four ABC transporters. In total, 60 significantly up-regulated genes (log2fold-change ≥ 2) and 39 significantly down-regulated genes (log2fold-change ≤ - 2) were identified by a transcriptome comparison between strains SCT-10-10-60 and ATCC 11443. KEGG pathway enrichment analysis revealed that "pyruvate metabolism" was significantly different (P < 0.05) between the two strains. The split genes and the differentially expressed genes involved in the "pyruvate metabolism" pathway are probably responsible for the increased L-lactic acid production by SCT-10-10-60. The genome and transcriptome sequencing information and comparison of SCT-10-10-60 with ATCC 11443 provide insights into the anabolism of L-lactic acid and a reference for improving L-lactic acid production using genetic engineering.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácido Láctico
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Genômica
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Lacticaseibacillus rhamnosus
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Transcriptoma
Idioma:
En
Revista:
Mol Genet Genomics
Assunto da revista:
BIOLOGIA MOLECULAR
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GENETICA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China