Cloning, purification and structure determination of the HIV integrase-binding domain of lens epithelium-derived growth factor.
Acta Crystallogr F Struct Biol Commun
; 74(Pt 3): 143-149, 2018 03 01.
Article
em En
| MEDLINE
| ID: mdl-29497017
ABSTRACT
Lens epithelium-derived growth factor (LEDGF)/p75 is the dominant binding partner of HIV-1 integrase in human cells. The crystal structure of the HIV integrase-binding domain (IBD) of LEDGF has been determined in the absence of ligand. IBD was overexpressed in Escherichia coli, purified and crystallized by sitting-drop vapour diffusion. X-ray diffraction data were collected at Diamond Light Source to a resolution of 2.05â
Å. The crystals belonged to space group P21, with eight polypeptide chains in the asymmetric unit arranged as an unusual octamer composed of four domain-swapped IBD dimers. IBD exists as a mixture of monomers and dimers in concentrated solutions, but the dimers are unlikely to be biologically relevant.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Integrase de HIV
/
Proteínas Adaptadoras de Transdução de Sinal
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Acta Crystallogr F Struct Biol Commun
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Reino Unido