Novel assay system for acidic Peptide:N-glycanase (aPNGase) activity in crude plant extract.

Uemura, Ryota; Ogura, Mikako; Matsumaru, Chihiro; Akiyama, Tsuyoshi; Maeda, Megumi; Kimura, Yoshinobu

Uemura, Ryota; Ogura, Mikako; Matsumaru, Chihiro; Akiyama, Tsuyoshi; Maeda, Megumi; Kimura, Yoshinobu.

Afiliação

Uemura R; a Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University , Okayama , Japan.
Ogura M; b Faculty of Agriculture , Okayama University , Okayama , Japan.
Matsumaru C; a Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University , Okayama , Japan.
Akiyama T; a Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University , Okayama , Japan.
Maeda M; a Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science , Okayama University , Okayama , Japan.
Kimura Y; b Faculty of Agriculture , Okayama University , Okayama , Japan.

Biosci Biotechnol Biochem ; 82(7): 1172-1175, 2018 Jul.

Article em En | MEDLINE | ID: mdl-29658432

Assuntos

Peptídeo-N4-(N-acetil-beta-glucosaminil) Asparagina Amidase/metabolismo; Extratos Vegetais/química; Sequência de Aminoácidos; Arabidopsis/química; Cromatografia Líquida/métodos; Corantes Fluorescentes/química; Glicopeptídeos/química; Glicopeptídeos/metabolismo; Solanum lycopersicum/química; Peptídeo-N4-(N-acetil-beta-glucosaminil) Asparagina Amidase/genética; Peptídeo-N4-(N-acetil-beta-glucosaminil) Asparagina Amidase/isolamento & purificação; Folhas de Planta/química; Plantas Geneticamente Modificadas; Especificidade por Substrato

Palavras-chave

Acidic PNGase; FNG: free N-glycan; Fuc: L-fucose; Gal: D-galactose; GlcNAc: N-acetyl-D-glucosamine; HPLC: high-performance liquid chromatography; Man: D-mannose; NeuNAc2Gal2GlcNAc2Man3GlcNAc1: NeuNAcα26Galß14GlcNAcß12Manα16(NeuNAcα26Galß14GlcNAcß12Manα13)Manß14GlcNAc; NeuNAc2Gal2GlcNAc2Man3GlcNAc2: NeuNAcα26Galß14GlcNAcß12Manα16(NeuNAcα26Galß14GlcNAcß12Manα13)Manß14GlcNAcß14GlcNAc; NeuNAc: N-acetylneuraminic acid; PA-: pyridylamino; PNGase-A: aPNGase from almond seed; PNGase-Le: aPNGase from tomato (Solanum lycopersium L.); PNGase: peptide:N-glycanase; PTC: plant complex type; RCA120: Ricinus communis agglutinin (120 kDa); RP-HPLC: reversed-phase HPLC; SF-HPLC: size-fractionation HPLC; Xyl: D-xylose; affinity chromatography; enzyme assay; free N-glycans; transgenic plant

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Peptídeo-N4-(N-acetil-beta-glucosaminil) Asparagina Amidase Idioma: En Revista: Biosci Biotechnol Biochem Assunto da revista: BIOQUIMICA / BIOTECNOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Japão

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