Establishment of a detection assay for DNA endonuclease activity and its application in the screening and prognosis of malignant lymphoma.
BMC Biochem
; 19(1): 6, 2018 07 31.
Article
em En
| MEDLINE
| ID: mdl-30064372
ABSTRACT
BACKGROUND:
Endonucleases play critical roles in maintaining genomic stability and regulating cell growth. The purpose of this study was to evaluate detection of endonuclease activity as an indicator in the early diagnosis and prognosis of lymphoma.RESULTS:
The method of endonuclease activity determination was successfully established and applied to compare cancer patient and control cohorts. Endonuclease activities of cancer tissues were significantly higher than those of adjacent control tissues (P < 0.001). We next investigated endonuclease activity in peripheral blood of enrolled patients and the controls, which were also significantly higher in patients than in controls (P = 0.015). Additionally, endonuclease activities were elevated in the metastasis subgroup compared with the non-metastasis subgroup(P = 0.038), whereas no significant difference was found between age(≤ 56y, > 56y) and gender (P = 0.736 > 0.05 and P = 0.635 > 0.05, respectively). Although there was no significant difference between control group with the non-metastatic cancer patients (P = 0.800 > 0.05), endonuclease activities were lower in the control group compared with the non-metastatic cancer patients with lymphoma (P = 0.033). The progression-free survival probability of patients with elevated R ratios(R ratio ≥ 1.4) was significantly lower than that of patients with lower R ratios (R ratio < 1.4).CONCLUSIONS:
An assay was established to detect the endonuclease activity,which might be useful for the prognosis of cancers, especially lymphoma.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biomarcadores Tumorais
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Desoxirribonuclease I
/
Ensaios Enzimáticos
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Linfoma
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
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Prognostic_studies
/
Screening_studies
Limite:
Female
/
Humans
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Male
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Middle aged
Idioma:
En
Revista:
BMC Biochem
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China