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Characterization of Recombinant Adeno-Associated Viral Transduction and Safety Profiles in Cardiomyocytes.
Ai, Jianzhong; He, Yong; Zheng, Mingxia; Wen, Yi; Zhang, Huan; Huang, Fangyang; Zhu, Ye.
Afiliação
  • Ai J; Institute of Urology, Department of Urology, West China Hospital, Sichuan University, Chengdu, China.
  • He Y; Department of Cardiology, West China Hospital, Sichuan University, Chengdu, China.
  • Zheng M; Department of Cardiology, West China Hospital, Sichuan University, Chengdu, China.
  • Wen Y; Department of Cardiology, West China Hospital, Sichuan University, Chengdu, China.
  • Zhang H; Department of Cardiology, West China Hospital, Sichuan University, Chengdu, China.
  • Huang F; Department of Cardiology, West China Hospital, Sichuan University, Chengdu, China.
  • Zhu Y; Department of Cardiology, West China Hospital, Sichuan University, Chengdu, China.
Cell Physiol Biochem ; 48(5): 1894-1900, 2018.
Article em En | MEDLINE | ID: mdl-30092576
BACKGROUND/AIMS: Cardiovascular diseases (CVD) are the leading causes for human mortality. However, the effective treatment for these diseases are still lacking. Currently, gene therapy could be a potential way for efficiently treating heart diseases. The aim of our study is to analyze the transduction efficacy and safety profile of recombinant adeno associated virus (AAV) serotype 9 for cardiomyocytes in vivo and in vitro. METHODS: We produced rAAV serotype 9 expressing enhanced green fluorescence protein (EGFP) driven by a cardiac troponin T (cTNT) promoter, and characterized its transduction efficiency in primary cultured cardiomyocytes in vitro, and in wild-type mouse heart tissue in vivo. RESULTS: Our data showed that rAAV9 efficiently transduced mouse cardiomyocytes in vitro. Following intravenous injection, rAAV9 could efficiently and safely transduce cardiomyocytes that are involved in heart diseases. CONCLUSION: Our findings suggested that rAAV9 can efficiently and safely transduce cardiomyocytes in vitro and/or in vivo. The rAAV9 serotype vector could constitute a powerful toolbox for future gene therapy of heart diseases.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transdução Genética / Dependovirus / Vetores Genéticos Tipo de estudo: Risk_factors_studies Limite: Animals / Humans Idioma: En Revista: Cell Physiol Biochem Assunto da revista: BIOQUIMICA / FARMACOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transdução Genética / Dependovirus / Vetores Genéticos Tipo de estudo: Risk_factors_studies Limite: Animals / Humans Idioma: En Revista: Cell Physiol Biochem Assunto da revista: BIOQUIMICA / FARMACOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: China