Response of Paracoccidioides lutzii to the antifungal camphene thiosemicarbazide determined by proteomic analysis.
Future Microbiol
; 13: 1473-1496, 2018 10.
Article
em En
| MEDLINE
| ID: mdl-30311782
ABSTRACT
AIM:
To perform the proteomic profile of Paracoccidioides lutzii after treatment with the compound camphene thiosemicarbazide (TSC-C) in order to study its mode of action.METHODS:
Proteomic analysis was carried out after cells were incubated with TSC-C in a subinhibitory concentration. Validation of the proteomic results comprised the azocasein assay, western blot and determination of the susceptibility of a mutant to the compound.RESULTS:
Proteins related to metabolism, energy and protein fate were regulated after treatment. In addition, TSC-C reduces the proteolytic activity of the protein extract similarly to different types of protease inhibitors.CONCLUSION:
TSC-C showed encouraging antifungal activity, working as a protease inhibitor and downregulating important pathways impairing the ability of the fungi cells to produce important precursors.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Paracoccidioides
/
Semicarbazidas
/
Terpenos
/
Proteômica
/
Antifúngicos
Idioma:
En
Revista:
Future Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Brasil