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Functional analysis of Salmonella Typhi adaptation to survival in water.
Kingsley, Robert A; Langridge, Gemma; Smith, Sarah E; Makendi, Carine; Fookes, Maria; Wileman, Tom M; El Ghany, Moataz Abd; Keith Turner, A; Dyson, Zoe A; Sridhar, Sushmita; Pickard, Derek; Kay, Sally; Feasey, Nicholas; Wong, Vanessa; Barquist, Lars; Dougan, Gordon.
Afiliação
  • Kingsley RA; Quadram Institute Bioscience, Norwich Research Park, Norwich, UK.
  • Langridge G; Quadram Institute Bioscience, Norwich Research Park, Norwich, UK.
  • Smith SE; The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
  • Makendi C; The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
  • Fookes M; The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
  • Wileman TM; The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
  • El Ghany MA; The Westmead Institute for Medical Research, The University of Sydney, Sydney, Australia and Marie Bashir Institute for Infectious Diseases and Biosecurity, The University of Sydney, Sydney, Australia.
  • Keith Turner A; Quadram Institute Bioscience, Norwich Research Park, Norwich, UK.
  • Dyson ZA; Department of Medicine, University of Cambridge, Addenbrooke's Hospital, Hills Road, Cambridge, UK.
  • Sridhar S; The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
  • Pickard D; Department of Medicine, University of Cambridge, Addenbrooke's Hospital, Hills Road, Cambridge, UK.
  • Kay S; The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
  • Feasey N; The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
  • Wong V; Liverpool School of Tropical Medicine, Liverpool, UK.
  • Barquist L; Department of Medicine, University of Cambridge, Addenbrooke's Hospital, Hills Road, Cambridge, UK.
  • Dougan G; Helmholtz Institute for RNA-based Infection Research, Würzburg, Germany.
Environ Microbiol ; 20(11): 4079-4090, 2018 11.
Article em En | MEDLINE | ID: mdl-30450829
Contaminated water is a major risk factor associated with the transmission of Salmonella enterica serovar Typhi (S. Typhi), the aetiological agent of human typhoid. However, little is known about how this pathogen adapts to living in the aqueous environment. We used transcriptome analysis (RNA-seq) and transposon mutagenesis (TraDIS) to characterize these adaptive changes and identify multiple genes that contribute to survival. Over half of the genes in the S. Typhi genome altered expression level within the first 24 h following transfer from broth culture to water, although relatively few did so in the first 30 min. Genes linked to central metabolism, stress associated with arrested proton motive force and respiratory chain factors changed expression levels. Additionally, motility and chemotaxis genes increased expression, consistent with a scavenging lifestyle. The viaB-associated gene tviC encoding a glcNAc epimerase that is required for Vi polysaccharide biosynthesis was, along with several other genes, shown to contribute to survival in water. Thus, we define regulatory adaptation operating in S. Typhi that facilitates survival in water.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Salmonella typhi / Viabilidade Microbiana / Água Doce Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: Environ Microbiol Assunto da revista: MICROBIOLOGIA / SAUDE AMBIENTAL Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Salmonella typhi / Viabilidade Microbiana / Água Doce Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: Environ Microbiol Assunto da revista: MICROBIOLOGIA / SAUDE AMBIENTAL Ano de publicação: 2018 Tipo de documento: Article