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Direct visualization of single-molecule membrane protein interactions in living cells.
Kim, Do-Hyeon; Park, Soyeon; Kim, Dong-Kyun; Jeong, Min Gyu; Noh, Jungeun; Kwon, Yonghoon; Zhou, Kai; Lee, Nam Ki; Ryu, Sung Ho.
Afiliação
  • Kim DH; Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
  • Park S; Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
  • Kim DK; School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Republic of Korea.
  • Jeong MG; Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang, Republic of Korea.
  • Noh J; Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
  • Kwon Y; Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
  • Zhou K; Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
  • Lee NK; School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang, Republic of Korea.
  • Ryu SH; Department of Chemistry, Seoul National University, Seoul, Republic of Korea.
PLoS Biol ; 16(12): e2006660, 2018 12.
Article em En | MEDLINE | ID: mdl-30543635
Interactions between membrane proteins are poorly understood despite their importance in cell signaling and drug development. Here, we present a co-immunoimmobilization assay (Co-II) enabling the direct observation of membrane protein interactions in single living cells that overcomes the limitations of currently prevalent proximity-based indirect methods. Using Co-II, we investigated the transient homodimerizations of epidermal growth factor receptor (EGFR) and beta-2 adrenergic receptor (ß2-AR) in living cells, revealing the differential regulation of these receptors' dimerizations by molecular conformations and microenvironment in a plasma membrane. Co-II should provide a simple, rapid, and robust platform for visualizing both weak and strong protein interactions in the plasma membrane of living cells.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Mapeamento de Interação de Proteínas / Imunoprecipitação / Análise de Célula Única Limite: Humans Idioma: En Revista: PLoS Biol Assunto da revista: BIOLOGIA Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Mapeamento de Interação de Proteínas / Imunoprecipitação / Análise de Célula Única Limite: Humans Idioma: En Revista: PLoS Biol Assunto da revista: BIOLOGIA Ano de publicação: 2018 Tipo de documento: Article