The ideal holding time for boar semen is 24â¯h at 17⯰C prior to short-cryopreservation protocols.
Cryobiology
; 86: 58-64, 2019 02.
Article
em En
| MEDLINE
| ID: mdl-30557556
ABSTRACT
Boar semen cannot be immediately cryopreserved, it need be hold at 17⯰C prior to cryopreservation, holding time has been used to improve cryopreserved boar semen, since holding time allows a prolonged interaction between spermatozoa and seminal plasma components. However, until now only few periods of holding time have been studied, and boar semen had been held at 17⯰C for 24â¯h to facilitate its manufacture. Thus, this experiment aims to study the effect several holding time (0, 4, 8, 12, 24, 28 and 32â¯h) on boar spermatozoa post-thawed (PT) characteristics. Fifteen sperm-rich fractions of ejaculate were extended in Beltsville Thawing Solution and storage at 17⯰C. After each holding time (0, 4, 8, 12, 24, 28 and 32â¯h), a sample was centrifuged, and sperm pellet was diluted in an extender composed of sugars, amino acids, buffers, 20% egg yolk (v/v), antibiotics, 2% glycerol as a cryoprotectant, and 2% methylformamide (v/v). Cryopreservation was performed with an automatic cryopreservation system. Cryopreserved boar semen was evaluated to spermatozoa kinetics, plasma and acrosomal membranes integrity, mitochondrial membrane potential, detection of superoxide anion, plasma membrane fluidity, and peroxidation. Twenty-four hours of holding increase total and progressive motility, rapid spermatozoa, and integrity of plasma and acrosome membranes. To mitochondrial membrane potential, 32â¯h is needed. However, holding time was not able to control the superoxide anion amount neither membrane lipid peroxidation, and had no effects on membrane fluidity. Thus, to reach the best results of PT boar semen the ideal holding time is 24â¯h.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sêmen
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Preservação do Sêmen
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Motilidade dos Espermatozoides
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Criopreservação
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Análise do Sêmen
Limite:
Animals
Idioma:
En
Revista:
Cryobiology
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Brasil